Journal of Shandong University (Health Sciences) ›› 2026, Vol. 64 ›› Issue (1): 65-73.doi: 10.6040/j.issn.1671-7554.0.2025.1424

• Preclinical Medicine • Previous Articles     Next Articles

Expression of GPX1 in lung cancer cell lines and its influence on the proliferation, migration, invasion abilities and apoptosis in lung adenocarcinoma cell lines

YIN Yue1,2, MO Zhenfei1,2, WU Peixin3, LIU Jinxia2, WEI Yuanhui3, REN Jiabo1,2, LI Chunsun2   

  1. 1. Chinese PLA Medical School, Beijing 100853, China;
    2. Department of Pulmonary and Critical Care Medicine, Chinese PLA General Hospital, Beijing 100853, China;
    3. School of Medicine, Nankai University, Tianjin 300071, China
  • Published:2026-01-27

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Abstract: Objective To investigate the expression characteristics, subcellular localization of glutathione peroxidase-1(GPX1)in different pathological types of lung cancer cell lines and the influence on the biological functions of lung adenocarcinoma cell lines. Methods The expressions of GPX1 at mRNA and protein levels in normal lung epithelial cells HPAEpic, lung adenocarcinoma cell line A549 and H292, lung squamous cell carcinoma cell line Calu1, large cell lung cancer cell line 95D, and small cell lung cancer cell line H446 were detected by quantitative real-time PCR and Western blotting, respectively. The expression differences of GPX1 between different pathological types of lung cancer cell lines and normal lung epithelial cells were compared through analysis of variance. The subcellular localization of GPX1 in lung adenocarcinoma cell lines was clarified by immunofluorescence staining. The lung adenocarcinoma cell lines H292 and A549 GPX1 stably knocked down cell strains were constructed, and the knockdown efficiency was verified at mRNA and protein levels, respectively. The proliferation, migration, invasion abilities and apoptosis of H292 and A549 cell strains were detected by CCK-8 assay, wound healing assay, Transwell assay and flow cytometry, respectively. Results The quantitative real-time PCR results showed that the expression of GPX1 was upregulated in lung adenocarcinoma cell line H292 compared with the normal lung epithelial cells HPAEpic(P=0.006). The results of Western blotting showed that compared with the normal lung epithelial cells HPAEpic, expression of GPX1 was upregulated in multiple lung cancer cell lines(A549, H292, Calu1, 95D, and H446)(P<0.001). The results of CCK-8, wound healing assay, Transwell assay and flow cytometry showed that after knockdown of GPX1, the proliferation(P<0.001), migration(P<0.001, P=0.002)and invasion abilities(P=0.039, P=0.014)of H292 and A549 cell strains were all weakened, while apoptosis rate increased(P=0.008, P=0.040). Conclusion The expression of GPX1 is highly upregulated in various pathological types of lung cancer cell lines, and its expression level is the highest among lung adenocarcinoma cell lines. GPX1 is mainly located in cytoplasm. Down-regulation of GPX1 expression can significantly inhibit the proliferation, migration and invasion abilities of A549 and H292 lung adenocarcinoma cell strains, and promote cell apoptosis.

Key words: Lung adenocarcinoma, Glutathione peroxidase-1, Proliferation, Migration, Invasion, Apoptosis

CLC Number: 

  • R574
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