Journal of Shandong University (Health Sciences) ›› 2025, Vol. 63 ›› Issue (4): 69-74.doi: 10.6040/j.issn.1671-7554.0.2024.0717

• Preclinical Medicine • Previous Articles    

Effect of α5-nAChR on the expression of c-Fos in nucleus ambiguus in chronic stress-induced lung adenocarcinoma mice

CAI Jiaying1, WANG Jingting1, WANG Zengping2, WANG Jing1, JIA Yanfei1, MA Xiaoli1,2   

  1. 1. Research Center of Basic Medicine, Central Hospital Affiliated to Shandong First Medical University, Jinan 250013, Shandong, China;
    2. Department of Medical Laboratory, Shandong Second Medical University, Weifang 261053, Shandong, China
  • Published:2025-04-08

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Abstract: Objective To investigate the effect of α5-nicotinic acetylcholine receptor(α5-nAChR)on the expression of c-Fos in nucleus ambiguus in chronic unpredictable mild stress(CUMS)-induced lung adenocarcinoma mice. Methods The expression and correlation between α5-nAChR and Ki67 in tumour tissues of the NC+CUMS group(n=6), the CUMS+CHRNA5+/+ group(n=6)and the CUMS+CHRNA5+/++Mec group(n=6)were examined using immunohistochemistry in lung adenocarcinoma mice. Immunofluorescence technique was used to detect the expression of c-Fos in nucleus ambiguus of the NC group(n=6), the NC+CUMS group(n=6), the CUMS+CHRNA5+/+ group and the CUMS+CHRNA5+/++Mec group(n=6)in lung adenocarcinoma mice. Results α5-nAChR was positively correlated with the expression of Ki67(P<0.05)and α5-nAChR mediates chronic stress-induced lung adenocarcinoma cell proliferation. α5-nAChR expression was positively correlated with the expression of c-Fos in nucleus ambiguus(P<0.05). Conclusion α5-nAChR mediates the expression of c-Fos in nucleus ambiguus in chronic stress-induced lung adenocarcinoma mice.

Key words: α5-nicotinic acetylcholine receptor, c-Fos, Nucleus ambiguus, Chronic stress, Lung adenocarcinoma, Mice

CLC Number: 

  • R734.2
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