Journal of Shandong University (Health Sciences) ›› 2023, Vol. 61 ›› Issue (8): 40-49.doi: 10.6040/j.issn.1671-7554.0.2022.1243

• 临床医学 • Previous Articles    

Expression and potential mechanism of VPS72 in lung adenocarcinoma/ squamous cell carcinoma by integrated bioinformatics analysis

MENG Jianli1, WANG Qinggang2   

  1. 1. Department of the Information Center;
    2. Department of Clinical Laboratory, Qilu Hospital(Qingdao), Cheeloo College of Medicine, Shandong University, Qingdao 266035, Shandong, China
  • Published:2023-08-30

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Abstract: Objective To explore the expression, prognostic relationship and potential mechanism of vacuolar protein sorting 72 homolog(VPS72)in lung adenocarcinoma/squamous cell carcinoma using multiple databases. Methods The expression level of VPS72 in lung adenocarcinoma/squamous cell carcinoma was analyzed based on TCGA data set, TIMER database and R language data package. The prognostic relationship and diagnostic value of VPS72 were evaluated with Kaplan-Meier Plotter and ROC curve online software. The interacting proteins and co-expressed genes of VPS72 were screened with STRING and LinkedOmics, and GO/KEGG functional enrichment analysis was performed with LinkedOmics. The correlation between the expression of VPS72 and immune cell infiltration was analyzed with TIMER. The correlation between VPS72 expression and different immune subtypes was analyzed in TISIDB database. Results VPS72 was highly expressed in lung adenocarcinoma/squamous cell carcinoma. Lung adenocarcinoma patients with high expression of VPS72 had significantly shorter overall survival(P=0.01), but there was no significant difference in the overall survival of lung squamous cell carcinoma patients with high expression of VPS72(P=0.17). PPI analysis showed that VPS72 interacted with 10 proteins, including H2AFZ, RUVBL1, DMAP1, and so on. GO/KEGG functional enrichment analysis showed that VPS72 was mainly involved in spliceosome and ribosome synthesis. The expression of VPS72 in lung adenocarcinoma was negatively correlated with the infiltration of a variety of immune cells, including B cells, CD8+T cells, CD4+T cells, macrophages, neutrophils and dendritic cells. In lung squamous cell carcinoma, VPS72 expression was negatively correlated with macrophages and neutrophils. In lung adenocarcinoma/squamous cell carcinoma, VPS72 expression was correlated with 6 immune subtypes. Conclusion The expression of VPS72 is significantly increased in lung adenocarcinoma/squamous cell carcinoma, which is related to the prognosis of lung adenocarcinoma and involved in a variety of pathways and immune cells. VPS72 may become a new target for prognosis prediction and treatment of lung adenocarcinoma/squamous cell carcinoma.

Key words: Lung adenocarcinoma, Lung squamous cell carcinoma, Vacuolar protein sorting 72 homolog, The cancer genome atlas, Immune infiltration

CLC Number: 

  • R734.2
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