CD151-MUT突变对肺腺癌细胞A549迁移的影响及机制

doi:10.6040/j.issn.1671-7554.0.2019.1257

Abstract

Abstract: Objective To study the effect of CD151-MUT on migration of lung adenocarcinoma cell line A549 and the mechanism involved. Methods pAAV-CD151, pAAV-CD151-MUT and pAAV-GFP were constructed and transfected into A549, and named as CD151 group, CD151-MUT group and GFP group. Meanwhile, Control group and Reagent group were set up. The migration of A549 was detected by modified Boyden chamber assay. Protein expression levels of CD151,p-focal adhesion kinase(FAK)and p-P130Cas were measured by Western blotting. Results (1)Compared with the Control group(1.03±0.15), GFP group(1.18±0.13)and Reagent group(1.22±0.11), the expression of CD151 protein was increased both in the CD151 group(2.23±0.22)and CD151-MUT group(2.15±0.17)with statistical difference(F=39.8,P<0.001), but there was no significant difference of the CD151 protein expression between CDC151 and CDC151-CDC151-MUT groups(q=1.007,P=0.628). (2) The CD151 group significantly promoted cells migration compared with the Control group, GFP group and Reagent group(F=15.3, P=0.007). However, compared with the CD151 group, the migration and invasion of A549 cells were decreased in CD151-MUT group(q=6.760,P=0.010). (3) Overexpression of the CD151 protein could promote the expressions of p-FAK and p-P130Cas proteins(F=16.3,P<0.001; F=10.9,P=0.001), while the protein expressions were reduced in the CD151-MUT group compared with CD151 group(q=8.620,P=0.010; q=6.859,P=0.009). Conclusion CD151-MUT mutation impairs the formation of CD151-integrin and inhibits the tumor metastasis. The CD151-α3/α6 integrins complex is functionally important.

Key words: Integrin, CD151, Tumor migration, FAK-P130Cas, Lung adenocarcinoma cell line A549

CLC Number:

R734.2

YANG Xiuting, LIU Qigong, ZUO Ping, LIU Zhengxiang, ZUO Houjuan. Effect of CD151-MUT mutation on migration of lung adenocarcinoma cell line A549 and its mechanism[J].Journal of Shandong University (Health Sciences), 2020, 58(3): 81-86.

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Effect of CD151-MUT mutation on migration of lung adenocarcinoma cell line A549 and its mechanism

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