Journal of Shandong University (Health Sciences) ›› 2024, Vol. 62 ›› Issue (4): 68-77.doi: 10.6040/j.issn.1671-7554.0.2024.0185

• Clinical Medicine • Previous Articles    

Umbilical cord mesenchymal stem cells sheets treated by small intestinal submucosa promote wound healing

SHEN Feifei, LI Dong, LIU Linghong, JU Xiuli   

  1. Department of Pediatrics, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Published:2024-05-16

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Abstract: Objective To explore the effect of extracellular matrix(ECM), especially the small intestinal submucosa(SIS)-treated umbilical cord mesenchymal stem cells(UC-MSCs)sheets on wound healing. Methods Primary UC-MSCs were isolated and cultured from umbilical cord tissues using the tissue block apposition method. Decellularized SIS was obtained using a chemical method, and recombinant human fibronectin(rFN), SIS, and Matrigel were immobilized on the surface of polystyrene(PS)cell culture dishes by physical adhesion. The effects of PS surface, rFN-PS surface, SIS-PS surface, and Matrigel-PS surface on the morphology of UC-MSCs were investigated, and the changes in gene expression were detected by RT-PCR, then changes in the secretion of extracellular vesicles(EVs)were detected using nanoparticle tracking analysis. Rat skin total excision wounds were treated with ECM-free, rFN-treated, SIS-treated, and Matrigel-treated UC-MSCs cell sheets, respectively, and the therapeutic effect was further verified by calculating the ratio of wound area to the original wound area. Results Compared with the PS surface, the morphology of UC-MSCs cultured on the SIS-PS surface was almost unaltered(P>0.05), and RT-PCR assay showed that gene expression of the SIS-PS surface-cultured UC-MSCs was enhanced, such as Nanog homeobox(NANOG, P=0.041), SRY-box transcription factor 2(SOX-2, P=0.009), octamer-binding transcription factor-4(OCT-4, P<0.001), interleukin-10(IL-10, P=0.049), indoleamine 2,3-dioxygenase(IDO, P=0.007), and transforming growth factor beta(TGF-β, P=0.046), meanwhile the ability of the SIS-PS surface-cultured UC-MSCs to secrete EVs was significantly increased(P<0.001). The results of the animal experiments revealed that the lowest percentage of wound to original wound area was 26.9%±6.1% in the group of animals treated with SIS-treated UC-MSCs cell sheets at day 7. Conclusion UC-MSCs cultured on the SIS-PS surface significantly improved immunosuppressive function and EVs secretion ability, and the corresponding cell sheets have a stronger ability to promote wound healing.

Key words: Mesenchymal stem cells, Small intestinal submucosa, Wound healing, Extracellular vesicles, Extracellular matrix

CLC Number: 

  • R318.08
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