Journal of Shandong University (Health Sciences) ›› 2023, Vol. 61 ›› Issue (6): 41-46.doi: 10.6040/j.issn.1671-7554.0.2023.0283

• 基础医学 • Previous Articles    

Mechanism of Fugan Huaqian Decoction inhibiting HSC-T6 activation via Wnt/β-catenin signaling pathway

XIAO Zhenghua1, SHIYI Shize2,3, YANG Qingwan2, LI Tingting2,4, LI Shanshan2, ZHONG Yan2   

  1. 1. Department of Gastroenterology, The Second Affliated Hospital of Guizhou University of Traditional Chinese Medicine, Guiyang 550001, Guizhou, China;
    2. The Second Clinical Medical College of Guizhou University of Traditional Chinese Medicine, Guiyang 550001, Guizhou, China;
    3. Department of Gastroenterology, Meishan Hospital of Traditional Chinese Medicine, Meishan 620000, Sichuan, China;
    4. Department of Traditional Chinese Medicine, First Peoples Hospital of Lanzhou New Area, Lanzhou New Area 730300, Gansu, China
  • Published:2023-06-06

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Abstract: Objective To explore the inhibitory effect of Fugan Huaqian Decoction on hepatic stellate cell line HSC-T6. Methods A total of 40 healthy and clean male SD rats were given equivalent amount of Fugan Huaxian Decoction by gavage to prepare drug-containing serum and normal serum. The effects of different drug-containing serum concentrations(5%, 8%, 10%, 15%, 20%)on the proliferation rate of HSC-T6 cells were detected with CCK8 assay to determine the optimal acting concentration. HSC-T6 cells were divided into blank group(15% fetal bovine serum), model group ［transforming growth factor-β1(TGF-β1)+15% normal serum］ and treatment group(TGF-β1+15% medicated serum). Protein and mRNA expressions of Wnt1, β-catenin and Cyclin D1 were detected with Western blotting and q-PCR, respectively. Expression of α-SMA was determined with immunofluorescence staining. Results In the treatment group, the number of cells decreased, the morphology turned round, the volume shrank, and the number of suspension cells increased. CCK8 assay showed that drug-containing serum inhibited TGF-β1-induced proliferation of HSC-T6 cells(P<0.01), and the best effect was observed at 15% and 20% concentration, but there was no significant difference between the two groups(P>0.05). Western blotting and q-PCR showed that compared with the model group, the treatment group had decreased mRNA and protein expressions of Wnt1, β-catenin and Cyclin D1(P<0.01). Immunofluorescence staining showed that the cytoplasmic expression of α-SMA increased in the model group, but decreased in the treatment group. Conclusion Serum containing Fugan Huaqian Decoction can inhibit HSC-T6 activation and proliferation, and decrease the protein and mRNA expressions of Wnt1, β-catenin and Cyclin D1 in activated HSC-T6 cells. Fugan Huaqian Decoction may prevent and treat hepatic fibrosis by inhibiting the activation of Wnt/β-catenin signaling pathway.

Key words: Fugan Huaqian Decoction, Hepatic fibrosis, Hepatic stellate cells, Wnt1, β-catenin

CLC Number: 

  • R575
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