Journal of Shandong University (Health Sciences) ›› 2019, Vol. 57 ›› Issue (6): 51-60.doi: 10.6040/j.issn.1671-7554.0.2019.423

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Effect of Fugan Huaxian Decoction on TGF-β1/Smad signaling pathway in rats with hepatic fibrosis

XIAO Zhenghua1, YANG Hui2, LEI Wei3, YANG Jun3, YI Xu4, TAN Minmin5   

  1. 1. Department of Gastroenterology, Second Clinical Medical College of Guizhou University of Traditional Chinese Medicine, Guiyang 550001, Guizhou, China;
    2. Department of Neurology, the Second Clinical Medical College of Guizhou University of Traditional Chinese Medicine, Guiyang 550001, Guizhou, China;
    3. Grade 2016 Postgraduate, Guizhou University of Traditional Chinese Medicine, Guiyang 550001, Guizhou, China;
    4. Central laboratory of the Second Clinical Medical College of GuiZhou University of 〓山〓东〓大〓学〓学〓报〓(医〓学〓版)57卷6期〓-肖政华, 等.扶肝化纤汤对肝纤维化大鼠TGF-β1/Smad信号通路的影响〓\=-Traditional Chinese Medicine, Guiyang 550001, Guizhou, China;
    5. Department of Respiratory Medicine, the Second Clinical Medical College of GuiZhou University of Traditional Chinese Medicine, Guiyang 550001, Guizhou, China
  • Published:2022-09-27

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Abstract: Objective To explorethe effect of Fugan Huaxian Decoction on transforming growth factor β1(TGFβ1)/Smad signaling pathway in rats with hepatic fibrosis(HF)and the mechanism of anti-hepatic fibrosis. Methods A total of 120 SD rats were randomly divided into control group, model group, colchicine group, and high, medium and low dose group of decoction. Except for the control group,the other groups established HF rat models of Qi deficiency, poison weakness and blood stasis under the guidance of traditional Chinese medicine(TCM)theory based on classical subcutaneous injection of carbon tetrachloride(CCl4). After 6 weeks, when the models were successfully established, the decoction groups received gastrogavage for 3 weeks, while the control group and model group were given equal volume of pure water for 3 weeks. Next day after the last gastrogavage, the serum aspartate aminotransferase(AST), alanine aminotransferase(ALT), albumin(ALB), hydroxyproline(Hyp),laminin(LN)and pre-collagen type Ⅲ(PⅢNP)levels were measured. The liver tissues were stained with HE and Mason stainings. The protein and mRNA expressions of TGF-beta1, Smad3 and Smad7 in liver tissues were detected with qRT-PCR and immunohistochemistry. Results Compared with the model group, decoction groups showed relieved hepatocyte injury, decreased collagen fiber proliferation, improvedhepatic fibrosis(χ2=54.722, P<0.01), and reduced proportion of liver collagen fibers(χ2=52.149, P<0.01). Compared with the control group, the model group had increased serum ALT(χ2=39.685, P<0.01), AST(F=659.984, P<0.01), Hyp(χ2=38.613, P<0.01), LN(χ2=50.057, P<0.01), PⅢNP(F=63.492, P<0.01), decreased ALB(χ2=51.908, P<0.01), increased expressions of TGF-β1(χ2=40.900, P<0.01), Smad3(χ2=38.253, P<0.01), TGF-β1 mRNA(F=92.304, P<0.01), Smad3 mRNA(F=119.563, P<0.01), and decreased Smad7(χ2=48.949, P<0.01)and Smad7 mRNA(F=42.120, P<0.01). Compared with the model group, the decoction groups inhibited the changes of the above indexes(P<0.01), especially the high dosage group(P<0.01). Conclusion The model of Qi deficiency, poison weakness and blood stasis complicated with HF was successfully established. Fugan Huaxian Decoction can protect hepatocytes, alleviate liver injury and inhibit HF, probably by regulatingthe expressions of TGF-beta1, Smad3 and Smad7.

Key words: Fugan Huaxian Decoction, TGF-β1/Smad signaling pathway, Hepatic fibrosis, Syndrome combination, Rat model

CLC Number: 

  • R574
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