Journal of Shandong University (Health Sciences) ›› 2021, Vol. 59 ›› Issue (8): 44-52.doi: 10.6040/j.issn.1671-7554.0.2021.0228

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Adiponectin alleviated angiotensin II induced myocardial hypertrophy through sFRP2 and the related pathways

MENG Tingting, WANG Shuya, WU Huihui, CHEN Jiamin, ZHENG Yan, LI Ying, SU Guohai   

  1. Department of Cardiology, Afflicted Central Hospital of Shandong First Medical University, Jinan 250013, Shandong, China
  • Published:2021-09-16

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Abstract: Objective To explore whether adiponectin(APN)involves in the regulation of angiotensin Ⅱ(Ang Ⅱ)-induced myocardial hypertrophy through secreted frizzled-related protein 2(sFRP2)and to determine the related pathways. Methods Neonatal rat ventricular myocytes(NRVMs)were isolated from 3-day-old Wistar rats to detect the expression of α-SMA, a NRVMs cytoskeleton protein with immunofluorescence. NRVMs were divided into control group, 1 nmol/L group, 10 nmol/L group, 100 nmol/L group and 500 nmol/L group to detect the effects of different concentrations of Ang Ⅱ on NRVMs. NRVMs were then divided into control group, Ang Ⅱ group, APN+Ang Ⅱ group, Ang Ⅱ+APN+si-sFRP2 group, Ang Ⅱ+APN+LiCl group, APN group, SP600125+Ang Ⅱ group and SB203580+Ang Ⅱ group to explore the effects of APN on Ang Ⅱ. The expression of sFRP2 and activation of Wnt/β-catenin and p38/JNK pathways in each group were determined with Western blotting. The indexes related to myocardial hypertrophy and sFRP2 mRNA in each group were determined with qPCR. Results ANP and BNP were increased(FANP=27.30, P=0.002; FBNP=38.18, P=0.002), expressions of p-p38 and p-JNK were increased(Fp-JNK=57.65, P<0.001; Fp-p38=8.880, P=0.018)and expression of sFRP2 was down-regulated(FAngⅡ=47.53, P<0.001)with Ang Ⅱ treatment for 24 hours compared with control group. APN pretreatment decreased the expressions of ANP and BNP(FANP=101.8, P<0.001; FBNP=51.14, P<0.001), up-regulated the expression of sFRP2(F=88.93, P<0.001), and inhibited the activation of Wnt/β-catenin(F=41.33, P=0.006)and p38/JNK pathways(Fp38=73.42, P<0.001; FJNK=39.28, P=0.002)compared with the Ang Ⅱ group. The treatment of p38/JNK pathway inhibitors achieved the effects of APN pretreatment(FANP=122.9, P<0.001; FBNP=202.3, P<0.001). Conclusion APN may inhibit Ang Ⅱ induced myocardial hypertrophy by up-regulating sFRP2 and inhibiting the activation of Wnt/β-catenin and p38/JNK pathways.

Key words: Myocardial hypertrophy, Angiotensin Ⅱ, Adiponectin, Secreted frizzled-related protein 2, p38 mitogen-activated protein kinases, c-Jun N-terminal kinase, Wnt/β-catenin

CLC Number: 

  • R542.2
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