Journal of Shandong University (Health Sciences) ›› 2020, Vol. 58 ›› Issue (2): 1-6.doi: 10.6040/j.issn.1671-7554.0.2019.1047

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6-Gingerol reduces atrial fibrosis induced by angiotensin Ⅱ in rats

ZHAO Weikun1, LYU Xiangwei2, WU Qi3, LU Pan1, PENG Li1, QIN Qiuyu1   

  1. 1. Health Care Ward, Affiliated Hospital of Guilin Medical University, Guilin 541001, Guangxi, China;
    2. The Second Ward in Cardiovascular Department, Affiliated Hospital of Guilin Medical University, Guilin 541001, Guangxi, China;
    3. Department of Physiology, Xuzhou Medicine University, Xuzhou 221004, Jiangsu, China
  • Online:2020-02-10 Published:2022-09-27

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Abstract: Objective To investigate the effect and mechanism of 6-Gingerol(6-G)on atrial fibrosis induced by angiotensin Ⅱ(Ang II)in rats. Methods Eighteen 8-week-old male SD rats were randomly divided into sham operation group, model group, and 6-G intervention + Ang II group, with 6 rats in each group. Rats in sham operation group received 2 mg/kg 0.9% sodium chloride injection in the tail vein. Rats in model group received 2 mg/kg AngⅡ injection 山 东 大 学 学 报 (医 学 版)58卷2期 -赵位昆,等.6-姜酚减轻血管紧张素Ⅱ诱导的大鼠心房纤维化 \=-to induce atrial fibrosis. Rats in 6-G intervention + AngⅡ group received 6 mg/kg 6-G injection, and then 2 mg/kg Ang II injection after 30 min. Drugs were continuously administered for 14 days, once a day. The cardiac function was observed with echocardiography, changes of myocardial injury markers were determined with ELISA, degree of atrial fibrosis was measured with Massons trichrome staining, and TGF-β1 and collagen-Ⅰ expressions were detected with Western blotting. Results There were no statistically significant differences in LVEF(F=1.500, P=0.255), LVFS(F=0.793, P=0.471), LVESD(F=0.104, P=0.902), LVEDd(P=0.566)CK-MB(F=0.089, P=0.915), and cTnT(F=0.181, P=0.836)among the three groups(P>0.05). Compared with sham operation group, the model group and 6-G intervention + Ang II group had severer atrial fibrosis, elevated collagen fraction(F=322.527), TGF-β1(F=44.537)and Collagen-Ⅰ (F=45.626), and the differences were statistically significant(P<0.05). Compared with sham operation group, the model group had comparable cardiac function and myocardial injury markers(P>0.017), but severer atrial fibrosis, elevated collagen fraction, TGF-β1 and Collagen-I, and the differences were statistically significant(P<0.017). Compared with the model group, the 6-G intervention + AngⅡ group had no statistically different cardiac function and myocardial injury markers(P>0.017), but reduced atrial fibrosis, decreased collagen fraction, TGF-β1 and Collagen-Ⅰ, and the differences were statistically significant(P<0.017). Conclusion 6-G can improve AngⅡ-induced atrial fibrosis by inhibiting TGF-β1 and Collagen-Ⅰ expressions.

Key words: 6-Gingerol, Angiotensin Ⅱ, Atrial Fibrosis, Transforming growth factor β1, Collagen type-Ⅰ

CLC Number: 

  • R542.2
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