Journal of Shandong University (Health Sciences) ›› 2023, Vol. 61 ›› Issue (1): 10-16.doi: 10.6040/j.issn.1671-7554.0.2022.0885

• 基础医学 • Previous Articles    

Inhibitory effect and mechanism of astragaloside Ⅱ on renal clear cell carcinoma cells

ZHAO Kai1, YIN Xinbao2, ZHANG Zongliang2, WANG Zhenlin2, ZHU Guanqun2, WANG Ke2   

  1. 1. Liaoning University of Traditional Chinese Medicine, Shenyang 116600, Liaoning, China;
    2. Department of Urology, Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong, China
  • Published:2023-01-10

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Abstract: Objective To investigate the effect of astragaloside Ⅱ(As Ⅱ)on the proliferation of renal clear cell carcinoma 786-O cells and its potential mechanism. Methods The inhibitory effect of As Ⅱ on the growth and colony formation of 786-O cells was detected by MTT assay and cloning assay. Cell flow cytometry was used to detect the apoptosis-inducing effect of AS Ⅱ. Subsequently, Western blotting was used to detect the changes of PI3K-AKT-mTOR pathway protein and apoptosis executioner protein in cells treated with different concentrations of As Ⅱ. After co-treatment of cells with PI3K-AKT-mTOR pathway activator IGF-1 and As Ⅱ, flow cytometry was used to detect the changes of apoptosis and further verify the role of PI3K-AKT-mTOR pathway in apoptosis induction. Results As Ⅱ inhibited the proliferation of 786-O cells in a concentration- and time-dependent manner, with an interaction between time and concentration(Ftime=513.00, P<0.001; Fconcentration=1 678.00, P<0.001; Ftime×concentration=18.23, P<0.001), and inhibited cell colony formation. As Ⅱ induced apoptosis and increased the expression of cleved caspase-3, an apoptotic executioner protein(P0 μmol/L As Ⅱ group vs 10 μmol/L As Ⅱ group=0.013 9,P0 μmol/L As Ⅱ group vs 20 μmol/L As Ⅱ group<0.001). As Ⅱ inhibited the phosphorylation of PI3K, AKT and mTOR proteins(p-PI3K: P0 μmol/L As Ⅱ group vs 20 μmol/L As Ⅱ group=0.032 4;p-mTOR: P0 μmol/L As Ⅱ group vs 10 μmol/L As Ⅱ group=0.004 1,P0 μmol/L As Ⅱ group vs 20 μmol/L As Ⅱ group<0.001;p-AKT:P0 μmol/L As Ⅱ group vs 10 μmol/L As Ⅱ group=0.003 2,P0 μmol/L As Ⅱ group vs 20 μmol/L As Ⅱ group=0.001 2). IGF-1 reversed the phosphorylation of AKT by As Ⅱ(P=0.006 8), and attenuated the apoptosis-inducing effect of As Ⅱ on 786-O cells. Conclusion As Ⅱ can inhibit the proliferation and colony formation of renal carcinoma 780-O cells, and up-regulate the expression of cleved caspase-3 by inhibiting the phosphorylation of PI3K-AKT-mTOR signaling pathway, which in turn induces apoptosis.

Key words: Clear cell renal carcinoma, Astragaloside Ⅱ, Cancer therapy, Apoptosis

CLC Number: 

  • R737.11
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