Journal of Shandong University (Health Sciences) ›› 2023, Vol. 61 ›› Issue (2): 110-116.doi: 10.6040/j.issn.1671-7554.0.2022.0673

• 公共卫生与管理学 • Previous Articles     Next Articles

Effects of chlorogenic acid on the structure and allergenicity of shrimp tropomyosin

QU Xin, LYU Xiaojing, CHAI Qing, JU Guangxiu, WANG Shuhui   

  1. Department of Physical and Chemical, Qingdao Municipal Center for Disease Control &
    Prevention /Qingdao Institute of Preventive Medicine, Qingdao 266033, Shandong, China
  • Published:2023-02-17

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Abstract: Objective To investigate the effects of chlorogenic acid(CA)on the structure and allergenicity of shrimp tropomyosin(TM), so as to reduce the sensitization of shrimp allergen and provide theoretical reference for controlling the risk of shrimp allergy. Methods Changes in the structure of covalently bound complexes between CA and TM were analyzed with polyacrylamide gel electrophoresis, intrinsic fluorescence, circular dichroism chromatography, and changes in the potential allergenicity was analyzed with ELISA and KU812 cells. Results The band moved slightly upward and molecular weight increased with higher CA concentration after different concentrations of CA interacted with shrimp TM. After 2.0 mg/mL CA covalently bound to TM, the content of free amino groups, available lysine, surface hydrophobicity, intrinsic fluorescence intensity, α-helix, IgE binding capacity of TM, β-hexosidase, and histamine decreased by 46.01%, 32.10%, 36.36%, 68.49%, 41.12%, 32.56%, 37.70% and 23.46%, respectively. Interleukin-4 and interleukin-13 also showed a downward trend. Conclusion Covalent binding of CA can alter the structure of TM, thereby reducing its allergenicity.

Key words: Metapenaeus ensis, Tropomyosin, Chlorogenic acid, Structure, Allergenicity

CLC Number: 

  • R574
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