Journal of Shandong University (Health Sciences) ›› 2020, Vol. 58 ›› Issue (6): 76-82.doi: 10.6040/j.issn.1671-7554.0.2020.105

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Expressions and clinical significance of Beclin-1, P62 and Ki67 in different parts of nasal polyps

SUN Wenkai1, SUN Hui2, WU Xinfang1, GAO Wei3, MENG Zhaotun4, LI Qin5   

  1. 1. School of clinical Medicine, Binzhou Medical University, Yantai 264000, Shandong, China;
    2. Weifang Clinical Medical College, Weifang Medical University, Weifang 261000, Shandong, China;
    3. Graduate School, Shandong First Medical University, Taian 271000, Shandong, China;
    4. Medical College, Qingdao University, Qingdao 266000, Shandong, China;
    5. Department of Otorhinolaryngology &
    Head and Neck Surgery, Linyi Peoples Hospital, Linyi 276000, Shandong, China
  • Published:2022-09-27

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Abstract: Objective To investigate the role and mechanism of autophagy and proliferation in the development of nasal polyps(NPs)by detecting the expressions and relevance of autophagic markers Beclin-1 and P62 and proliferator Ki67 in the pedicle and body of NPs. Methods NP tissues of 46 patients with chronic rhinosinusitis with nasal polyps(CRSwNP)were collected during July 2018 and May 2019 in Linyi Peoples Hospital as experimental group(pedicle subgroup and body subgroup), and normal nasal septum mucosa of 10 patients with septum deviation were taken as control group. Samples in the experimental group were stained with hematoxylin-eosin(HE)and pathological types were determined based on eosinophil infiltration. The protein expressions of Beclin-1, P62 and Ki67 in the two subgroups and control group were detected with immunohistochemistry(IHC). The expressions of Beclin-1 and P62 were detected with Western blotting. The correlation among Beclin-1, P62 and Ki67 was analyzed with Spearman rank correlation assay. Results There was no significant association between expressions of Beclin-1, P62 and Ki67 and pathological types of NPs(P>0.05). Beclin-1 expression was significantly lower in pedicle subgroup than in body subgroup, and was lower than that in the control group(H=71.835, P<0.05). P62 expression was significantly higher in pedicle subgroup than in body subgroup, and was higher than that in the control group(H=74.666, P<0.05). Ki67 expression was significantly higher in pedicle subgroup than in body subgroup, and was higher than that in the control group(H=76.716, P<0.05). Beclin-1 was negatively correlated with P62(rs=-0.674, P<0.05). Ki67 was negatively correlated with Beclin-1(rs=-0.640, P<0.05)but positively correlated with P62(rs=0.708, P<0.05). Conclusion Expressions of Beclin-1, P62 and Ki67 are different between pedicle and body in NPs, and there is negative correlation between Beclin-1, P62 and Ki67. The expressions of Beclin-1 and P62 are lower while expression of Ki67 is higher in pedicle than in body, indicating that Ki67 may promote the occurrence and development of NPs.

Key words: Nasal polyps, Autophagy, Beclin-1, P62, Ki67, Pedicle of nasal polyps, Body of nasal polyps

CLC Number: 

  • R765
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