JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2014, Vol. 52 ›› Issue (9): 39-43.doi: 10.6040/j.issn.1671-7554.0.2014.170

Previous Articles     Next Articles

COX-2 specific inhibitor induced apoptosis and autophagy in Hep-2 cells in vitro

ZHAO Yongqiang1,2, XIE Xiaoye2, CHEN Xuemei1, FENG Huiwei1, JIA Tao1, ZHANG Hui1, FAN Xianliang1   

  1. 1. Department of Otorhinolaryngology, the Second Hospital of Shandong University, Jinan 250033, Shandong, China;
    2. School of Medicine, Shandong University, Jinan 250012, Shandong, China
  • Received:2014-03-26 Revised:2014-07-01 Online:2014-09-10 Published:2014-09-10
  • Contact: 范献良。E-mail:sdentfan@126.com E-mail:sdentfan@126.com

Abstract: Objective To investigate the ability of celecoxib inducing apoptosis in Hep-2 cells and its possible mechanisms, as well as to observe the autophagy of the cells. Methods MTT was used to observe the proliferation of Hep-2 cells treated with celecoxib at different doses(0-100 μmol/L) and for different hours(0-72 hours). Cell ultrastructure was observed by electron microscope. Hep-2 cells were treated with celecoxib at different doses and for different hours and then the cell apoptosis rate was measured by flow cytometry. AIF expression was examined by Western blotting. Results Celecoxib induced a time- and dose-dependent growth inhibition in Hep-2 cells. It also induced the apoptosis of Hep-2 cells in a dose-dependent manner. No significant difference existed in the apoptosis rate of the cells treated by celecoxib for 72 and 48 hours(P>0.05). Autophagy was observed in Hep-2 cells treated by celscoxib for 72 hours. Celecoxib showed the ability of transporting AIF from mitochondria to cell nucleus. Conclusion Celecoxib can induce cell apoptosis, in which AIF mechanism may be involved. Autophagy induced by celecoxib may protect Hep-2 cells against apoptosis.

Key words: Hep-2 cells, Apoptosis, Celecoxib, Autophagy, Cyclooxygenase-2

CLC Number: 

  • R739.65
[1] Berg J, Christoph T, Bodenteich A, et al. Heterogeneous distribution of COX-2 over-expression in human colon carcinoma cells[J]. Adv Exp Med Biol, 1997, 433(2):327-330.
[2] Perez-Ruiz E, Cazorla O, Redondo M, et al. Immunohistochemical expression of cyclooxygenase-2 in patients with advanced cancer of the larynx who have undergone induction chemotherapy with the intention of preserving phonation[J]. Clin Transl Oncol, 2012, 14(9):682-688.
[3] 孙希才, 葛荣明, 董庆汉, 等. 环氧化酶-2在喉鳞状细胞癌中的表达及其与微血管密度的关系[J]. 临床耳鼻咽喉科学杂志, 2005, 19(21):967-970. SUN Xicai, GE Yongming, DONG Qinghan, et al. The expressiong of cyclooxygenase-2 in laryngeal squamous cell carcinoma and its relationship with MVD[J]. J Clin Otorhinolarvnaol (China), 2005, 19(21):967-970.
[4] Aline C A, Fernanda S G, Felipe F S, et al. Effects of celecoxib treatment over the AKT pathway in head and neck squamous cell carcinoma[J]. J Oral Pathol Med, 2013, 42(10):793-798.
[5] Cho E I, Kowalske D P, Sasaki C T, et al. Tissue microarray analysis reveals prognostic significance of COX-2 expression for local relapse in T1-2N0 larynx cancer treated with primary radiation therapy[J]. Laryngoscope, 2004, 114(11):2001-2008.
[6] Wang Y J, Niu X P, Yang L, et al. Effects of celecoxib on cycle kinetics of gastric cancer cells and protein expression of cytochrome C and Caspase-9[J]. Asian Pac J Cancer Prev, 2013, 14(4):2343-2347.
[7] Bocca C, Bozzo F, Cannito S, et al. Celecoxib inactivates epithelial-mesenchymal transition stimulated by hypoxia and/or epidermal growth factor in colon cancer cells[J]. Mol Carcinog, 2012, 51(10):783-785.
[8] Ramer R, Walther U, Borchert P, et al. Induction but not inhibition of COX-2 confers human lung cancer cell apoptosis by celecoxib[J]. J Lipid Res, 2013, 54(11):3116-3129.
[9] Jeon Y W, Suh Y J. Synergistic apoptotic effect of celecoxib and luteolin on breast cancer cells[J]. Oncol Rep, 2013, 29(2):819-825.
[10] Huang H, Pierstorff E, Osawa E, et al. Active nanodiamond hydrogels for chemotherapeutic delivery[J]. Nano Letters, 2007, 7(11):3305-3314.
[11] Joza N, Susin S A, Daugas E, et al. Essential role of the mitochondrial apoptosis-inducing factor in programmed cell death[J]. Nature, 2001, 410(6828):549-554.
[12] Winkel R R, Kalhauge A, Fredfeldt K E. The useful-ness of ultrasound colour-doppler twinkling artefact for detecting urolithiasis compared with low dose nonenhanced computerized tomography[J]. Ultrasound Med Biol, 2012, 38(7):1180-1187.
[13] Zhou R, Zhang L, Wang R, et al. Effect of celecoxib on proliferation, apoptosis, and survivin expression in human glioma cell line U251[J]. Chin J C, 2010, 29(3):294-299.
[14] Mizushima N, Levine B, Cuervo A M, et al. Autophagy fights disexse through cellular self-digestion[J]. Na-ture, 2008, 451(7182):1069-1075.
[15] Zou Z, Yuan Z, Zhang Q, et al. Aurora kinase A inhibition-induced autophagy triggers drug resistance in breast cancer cells[J]. Autophagy, 2012, 8(12):1798-1810.
[1] LU Xiang-Dong, YANG Wei, XU Guang-Meng, QU Yuan-Ming. Expression and role of PPAR-γin meningiomas and troglitazone induced meningiomas cell apoptosis in vitro [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2209, 47(6): 65-.
[2] SUO Dongyang, SHEN Fei, GUO Hao, LIU Lichang, YANG Huimin, YANG Xiangdong. Expression and mechanism of Tim-3 in animal model of drug-induced acute kidney injury [J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 1-6.
[3] ZHAO Shanshan, LI Xiaomei, LIANG Ting, ZHANG Chao, SONG Jing, HOU Guihua. Impact of autophagy on targeting imaging of allorejection with 125I-anti-TLR5 [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(9): 46-52.
[4] YIN Lei, YIN Rui, LI Wenjia, LIU Shuai, L(¨overU)Jiaju. CYLD improves bladder cancer gemcitabine chemosensitivity via suppressing autophagy [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(8): 1-6.
[5] ZHANG Liang, XU Min, ZHUANG Xianghua, LOU Fuchen, LOU Nengjun, LÜ Li, GUO Wenjuan, ZHENG Fengjie, CHEN Shihong. An investigation of endoplasmic reticulum stress and apoptosis in diabetic peripheral neuropathy [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(8): 13-17.
[6] LI Rui, MA Weihong, REN Manyi, ZHAO Mengmeng, JIANG Shan, JU Yuanyuan, GUO Ying, SUN Zhaohui, SUI Shujian. Correlation between TWEAK and cardiac remodeling in essential hypertensive patients [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(5): 49-55.
[7] GUO Hehe, SUN Zhiqiang, LIU Yanjuan, LIU Yichen, LI Guang, ZHENG Fang. Norcantharidin influences the expression of Notch signal pathway in multiple myeloma U226 cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(3): 32-37.
[8] TU Yunhua, CHEN Liyuan, WANG Xi, ZHOU Ying, KANG Yingqian, XUE Yuecui, RONG Dongyun, YE Zhenyuan, CAO Yu. Effect of ar-turmerone derivatives on proliferation and apoptosis in human melanoma WM35 cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(2): 68-73.
[9] GONG Luwei, ZHOU Lizhen, SU Guohai. Perindopril ameliorates cardiac dysfunction via Akt-FoxO1 pathway in diabetic cardiomyopathy [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(10): 65-70.
[10] CHEN Qi, LI Jiaqi, GUO Zhibin, JIA Jingyu, HE Linsheng. Role of methylation status of the cyclooxygenase-2 gene promoter in endometriosis [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(1): 44-48.
[11] ZHAO Lu, JIAO Jun, ZHANG Teng, JIAO Xinlin, CUI Baoxia. Expression of LXRα in ovarian carcinoma and effects of T0901317 on SKOV3 cell line [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(1): 49-53.
[12] ZHANG Kun, ZHAGN Xiaoguo, CUI Xingxing, CHEN Shijun. Expression of apoptosis-related genes in peripheral blood mononuclear cells of patients with HBV infection and the relationship with chronicity of hepatitis B [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(7): 38-42.
[13] JU Yuanyuan, REN Manyi, LI Rui, ZHAO Mengmeng, SUI Shujian. Tumor necrosis factor-like weak inducer of apoptosis promotes expression of matrix metalloproteinase 2 and collagen Ⅰ in rat cardiac fibroblasts via the ERK1/2 signaling pathway [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(5): 23-28.
[14] LI Hongzhi, LIU Jing, SONG Yan, CHI Lingyi, LIU Yuguang. Role of liraglutide in the repair of spinal cord injury [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(4): 1-5.
[15] REN Baoxin, MA Yunfeng, LIU Dianwei, LI Zhuo, JIANG Yong. Effects of Wnt3a on neuronal autophagy and apoptosis in early brain injury after subarachnoid hemorrhage in rats [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(10): 11-15.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!