Journal of Shandong University (Health Sciences) ›› 2019, Vol. 57 ›› Issue (12): 46-51.doi: 10.6040/j.issn.1671-7554.0.2019.886

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Biological effects of ubenimex on ovarian cancer A2780 cell

GUAN Hongwei, LI Juan, SUN Rui, LIU Jie, LI Changzhong   

  1. Department of Gynecology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China
  • Published:2022-09-27

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Abstract: Objective To investigate the effect of ubenimex on cell proliferation, cell cycle, apoptosis, autophagy, migration and invasion in ovarian cancer A2780 cell and its possible mechanism. Methods CCK-8 assay was used to analyze the effect of ubenimex on proliferation of A2780 cell. Transwell assay was performed to detect the effect of ubenimex on migration and invasion of A2780 cell. Flow cytometry was used to analyze the cell apoptosis and cell cycle. Western blotting was performed to investigate the protein expression levels of APN, AKT, p-AKT, LC3-B, and p62. Results (1)Ubenimex inhibited A2780 cell proliferation(Fconcentration=812.56,P<0.001;Ftime=8.58,P=0.010;Fconcentration×time=4.00,P=0.027), migration(t=56.9, P<0.001)and invasion(t=11.8, P<0.001), increased the cell proportion in G2/M phase (t=7.9, P=0.016)and decreased the cell proportion in G1 phase (t=14.8, P=0.005)which led to G2/M cell cycle arrest. (2)Ubenimex induced A2780 cell apoptosis(P=0.002), and cell apoptosis was increased when ubenimex was combined with AKT inhibitor(P=0.007). (3)Ubenimex reduced the APN expression, but increased p-AKT, LC3B and p62 expressions. Conclusion Ubenimex may be a treatment or adjuvant therapy for ovarian cancer, and its therapeutic effect can be enhanced in combination with AKT inhibitor.

Key words: Ovarian cancer, Ubenimex, Proliferation, Invasion, Apoptosis, Autophagy

CLC Number: 

  • R737.31
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