Journal of Shandong University (Health Sciences) ›› 2020, Vol. 58 ›› Issue (2): 7-12.doi: 10.6040/j.issn.1671-7554.0.2019.1046

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Effects of interferon-stimulated gene GPR135 on the replication of vesicular stomatitis virus

LYU Yahui1, DOU Chunhui2, LI Fan1, LI Daqi2   

  1. 1. Clinical College, Weifang Medical University, Weifang 261053, Shandong, China;
    2. Department of Hematology, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, Shandong, China
  • Online:2020-02-10 Published:2022-09-27

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Abstract: Objective To investigate the expression changes of G protein-coupled receptor(GPCR)135 in pathogen infection and its effects on the replication of vesicular stomatitis virus(VSV). Methods The expressions of GPR135 in mouse peritoneal macrophages(PEMs)in the cases of pathogen infection and interferon stimulation were detected with real-time fluorescent quantitative polymerase chain reaction(Q-PCR). The eukaryotic expression vector was constructed to overexpress GPR135 in HEK-293T cells. The effects of GPR135 overexpression on VSV replication were examined. Results In pathogen infection, GPR135 expression was increased(P<0.05). Interferon stimulation also led to an increase of GPR135 expression(P<0.05). After Stat1 interference, viral infection and interferon stimulation failed to upregulate Gpr135 expression. After GPR135 overexpression in HEK-293T cells, RNA replication of VSV was reduced(P<0.05), and expression of VSV glycoprotein(VSV-G)was significantly decreased. Conclusion In pathogen infection, interferon up-regulates GPR135 through the JAK-STAT signaling pathway and Gpr135 can be regarded as an interferon-stimulated gene. Overexpression of GPR135 in HEK-293T cells inhibits VSV replication.

Key words: G protein-coupled receptors, Interferon-stimulated genes, G protein-coupled receptor 135, Vesicular stomatitis virus

CLC Number: 

  • R574
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