Journal of Shandong University (Health Sciences) ›› 2018, Vol. 56 ›› Issue (5): 46-51.doi: 10.6040/j.issn.1671-7554.0.2018.171

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Effects of simvastatin on TGF-β induced epithelial-to-mesenchymal transition in ovarian cancer

HE Pengjuan1, YAN Lei2, FAN Mingjun1, LIU Xiangbin1, ZHAO Xingbo1   

  1. 1. Department of Obstetrics and Gynecology, Shandong Provincial Hospital Affilicated to Shandong University;
    2. Department of Gynecology, Hospital for Reproductive Medicine Affiliated to Shandong University, Jinan 250021, Shandong, China
  • Received:2018-02-01 Published:2022-09-27

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Abstract: Objective To investigate the effects of simvastatin on epithelial-to-mesenchymal transition(EMT)induced by transforming growth factor-β(TGF-β)in ovarian cancer cells(Skvo3 and A2780). Methods The ovarian cancer cells were divided into the control group, simvastatin group, TGF-β group, and simvastatin+TGF-β group. The effects of simvastatin and TGF-β at different concentrations on the cell proliferation were detected with CCK-8. The migration and invasion ability were assessed with wound healing assay and Transwell assay respectively. The changes of EMT and signaling pathway were determined with Western blotting. Results In Skvo3 and A2780 cells, simvastatin reduced cell proliferation and TGF-β-promoted cell proliferation, decreased cell migration and invasion, and reduced TGF-β-induced cell migration and invasion, and reversed EMT and TGF-β-induced EMT. Further experiments showed that simvastatin could reduce the expression of sperm-associated antigen 9(SPAG9)/c-Jun N-terminal kinase(JNK)pathway and reverse TGF-β-induced SPAG9 /JNK pathway(P<0.05). Conclusion Simvastatin can reduce the proliferation, migration, invasion and EMT induced by TGF-β in ovarian cancer cells, possibly by regulating SPAG9 / JNK pathway.

Key words: Ovarian cancer, Simvastatin, Transforming growth factor-β, Epithelial-to-mesenchymal transition, SPAG9/JNK pathway

CLC Number: 

  • R737.31
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