Journal of Shandong University (Health Sciences) ›› 2018, Vol. 56 ›› Issue (6): 6-12.doi: 10.6040/j.issn.1671-7554.0.2017.1234

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Protective effect of flavonoid components extracted from Diopyros Kaki on oxidative stress induced by OGD/R in HT22 cells

CUI Chunying, SHEN Chao, HONG Yan, CHEN Jian, LIU Xueping   

  1. Department of Senile Neurology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China
  • Published:2022-09-27

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Abstract: Objective To investigate the protective effect of flavonoid components extracted from Diopyros Kaki(FLDK)on oxidative stress induced by oxygen glucose deprivation/reperfusion(OGD/R)in HT22 cells. Methods HT22 cells were randomly divided into normal control group(Control group), OGD/R group, OGD/R+FLDK treatment group(OGD/R+FLDK group), OGD/R +FLDK+transcription factor NF-E2 related factors(Nrf2)gene knockdowm group(OGD/R+FLDK+si-Nrf2 group). OGD/R model of HT22 cells were established by oxygen glucose deprivation for 3 h followed by a 24 h reperfusion; FLDK was added at the beginning of the OGD, and continued to the end of reperfusion; using si-Nrf2 to block the Nrf2 pathway. CCK-8 assay was used to detect cell viability; lactate deoxidizing enzyme(LDH)assay was used to detect the cell injury rate; related kits were used to detect the MDA content, 山 东 大 学 学 报 (医 学 版)56卷6期 -崔春英,等.柿叶黄酮提取物对氧糖剥夺/复糖复氧损伤的HT22细胞氧化应激的保护作用 \=-SOD and GSH-Px activity; DCFH-DA fluorescent probe was used to detect the reactive oxygen species(ROS)levels; Western blotting was used to detect the nuclear-Nrf2 and HO-1 proteins expression. Results Compared with the control group, the cell activity was decreased(P<0.001); the cell injury rate was increased(P<0.001); the ROS and MDA content were increased(P<0.001); the antioxidant enzyme SOD and GSH-Px activity were decreased(P<0.001); HO-1 protein expression was increased(P=0.009)and the nuclear-Nrf2 protein expression was increased(P=0.016)in the OGD/R group. Compared with the OGD/R group, the cell activity was increased(P<0.001), the cell injury rate was decreased(P<0.001), the ROS and MDA content were decreased(P<0.001), the antioxidant enzyme SOD and GSH-Px activity were increased(P<0.001), the HO-1 protein expression was increased(P<0.001)and nuclear-Nrf2 protein expression was increased(P=0.005). Compared with the OGD/R+FLDK group, the HO-1 protein expression was decreased(P<0.001),the nuclear-Nrf2 protein was decreased(P=0.001), the ROS level was increased(P<0.001)and the cell activity was decreased(P<0.001). Conclusion FLDK can attenuate the oxidative stress in HT22 cells induced by OGD/R injury, and it may be associated with the activation of the Nrf2/HO-1 signaling pathway.

Key words: Diopyros Kaki, Flavonoid components, HT22 cells, Oxygen glucose deprivation, Nrf2, Signaling pathway

CLC Number: 

  • R743
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