JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2014, Vol. 52 ›› Issue (10): 103-107110.doi: 10.6040/j.issn.1671-7554.0.2014.422

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Microsatellite polymorphism of Candida albicans isolated from invasive infection sources in Guizhou region, China

WANG Meizhu1, LIU Shuiqing2, MA Kai3, LIU Juan3, LIU Taohua1, BAI Fengyan4, KANG Yingqian1   

  1. 1. Department of Microbiology, Guiyang Medical College, Guiyang 550004, Guizhou, China;
    2. Department of Infectious Disease, the Fifth People's Hospital of Guiyang, Guiyang 550004, Guizhou, China;
    3. Department of Clinical Laboratory, Second Affiliated Hospital of Guiyang College of Traditional Chinese Medicine, Guiyang 550003, Guizhou, China;
    4. State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
  • Received:2014-06-27 Revised:2014-09-17 Online:2014-10-10 Published:2014-10-10

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Abstract: Objective To survey the microsatellite polymorphism of Candida albicans(C.albicans) strains isolated from invasive infection sources in Guizhou region. Methods Ninety-five independent C.albicans strains isolated from invasive infection sources in Guizhou region were investigated based on single-strand conformation polymorphisms (SSCP) and GeneScan analysis. Genotypes were identified by microsatellite locus CAI polymorphism analysis. The polymorphic information content (PIC) and Nei's genetic distance were computed by software PowerMarker V3.25. In addition, phylogenetic tree was constructed based on UPGMA methods. Results Among the 95 C.albicans strains isolated from invasive infection sources, 40 distinct CAI genotypes with various patterns were identified by GeneScan analysis, and there were 7 novel CAI microsatellite genotypes which were identified in Guizhou province. The PIC was0.904 6. Seven clusters were divided in the UPGMA phylogenetic tree with Nei's the genetic distance, in which the strains from chronic obstructive pulmonary disease and AIDS were scattered in different clusters, while strains from tumor source were gathered in some relatively close clusters. Conclusion The C.albicans strains isolated from various invasive disease sources shows abundant genetic diversity characteristics. The CAI microsatellite genotypes of C.albicans from different sources are correlated to their micro-ecological evolution evidence.

Key words: Invasive infection sources, CAI microsatellite genotype, Candida albicans Guizhou

CLC Number: 

  • R379
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