JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (5): 29-33.doi: 10.6040/j.issn.1671-7554.0.2015.700

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Screening and separation of anti-tuberculosis active substances from Actinomycete co-evolution with Spongilla

FENG Lu1, MENG Fanchao2   

  1. 1. Department of Clinical Medicine;
    2. Department of Imaging, The Second Hospital of Shandong University, Jinan 250033, Shandong, China
  • Received:2015-07-26 Online:2016-05-16 Published:2016-05-16

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Abstract: Objective To screen and separate anti-tuberculosis active substances from Actinomycete metabolite co-evolutionary with Spongilla. Methods The monoclonal colony of different kinds of actinomycete co-evolutionary with Spongilla from the South China Sea were separated by the plate dilute method, and the anti-tuberculosis ability of the cultured supernatant was detected. Aaccording to molecular weight and polarity, the anti-tuberculosis active substances of strains were selected out and cultured for further separation by HPLC. Meanwhile, the genome DNA of NH128 strains were extracted and the 16S rRNA were amplified by PCR for sequencing. Results Of the 416 strains, 6 positive strains, in which the supernatant of NH128 strain showed a significant anti-tuberculosis H37Rv activity, were selected out. After the further separation, the component F3 and its sub-fraction S3, whose MIC50 component was about 3 μg/mL, of the supernatant of NH128 strain showed strong anti-tuberculosis activities. The NH128 strain was highly homologous with Streptomyces Aclinomycetales bacterium(R03 AY944262)in 99%. Conclusion Actinomycete co-evolutionary with spongilla in South China Sea have some anti-tuberculosis substances, and NH128 strain may be the variant of Aclinomycetales bacterium(R03 AY944262).

Key words: Spongilla, Anti-tuberculosis, High performance liquid chromatography, Co-evolution, Actinomycete

CLC Number: 

  • R379.9
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