雄激素阻断对膀胱癌UM-UC-3细胞自噬与凋亡的影响

doi:10.6040/j.issn.1671-7554.0.2017.251

摘要/Abstract

摘要： 目的探讨雄激素阻断对膀胱癌UM-UC-3细胞自噬与凋亡的影响。方法以双氢睾酮或比卡鲁胺激活或抑制UM-UC-3细胞AR信号通路,以雷帕霉素或氯喹进一步诱导或抑制UM-UC-3细胞自噬水平,根据以上不同处理条件进行相应分组。采用Hoechst染色、流式细胞仪和Western blotting检测UM-UC-3细胞凋亡;采用透射电镜、激光共聚焦显微镜和Western blotting检测UM-UC-3细胞自噬水平。结果雄激素去除或者AR阻断剂比卡鲁胺可以增强膀胱癌UM-UC-3细胞自噬和凋亡水平;雄激素阻断对UM-UC-3细胞的凋亡促进作用可以被氯喹抑制,并且可以被雷帕霉素增强。结论雄激素去除和AR阻断剂比卡鲁胺可以促进UM-UC-3细胞凋亡,这与其诱导细胞发生自噬相关。

关键词: 膀胱癌, 雄激素受体, 比卡鲁胺, 自噬, 凋亡

Abstract: Objective To explore the effect of androgen ablation and androgen receptor competitor biclutamide on autophagy and apoptosis in bladder cancer UM-UC-3 cells. Methods DHT and bicalutamide were respectively used to activate or inhibit androgen receptor signaling in UM-UC-3 cells. In addition, rapamycin and chloroquine were respectively used to induce or inhibit autophagy in UM-UC-3 cells. Hoechst staining, flow cytometry and Western blotting were used to observe the apoptotis. Autophagy flux was detected and quantified under transmission electronic microscope and confocal fluoresence microscope. Results Elevated autophagy and apoptosis levels were detected in UM-UC-3 cells after androgen ablation and androgen receptor competitor bicalutamide treatment. In addition, apoptosis induced by bicalutamide could be rescued by chloroquine and enhanced by rapamycin. Conclusion Androgen ablation and androgen receptor competitor bicalutamide promote apoptosis in UM-UC-3 cells due to autophagy induction.

Key words: Bladder cancer, Androgen receptor, Bicalutamide, Autophagy, Apoptosis

中图分类号:

R737.14

蒿魁元,赵圣,张宇,崔迪,荆翌峰,夏术阶,韩邦旻. 雄激素阻断对膀胱癌UM-UC-3细胞自噬与凋亡的影响[J]. 山东大学学报 (医学版), 2018, 56(3): 41-47.

HAO Kuiyuan, ZHAO Sheng, ZHANG Yu, CUI Di, JING Yifeng, XIA Shujie, HAN Bangmin. Effect of androgen blockade on autophagy and apoptosis in bladder cancer UM-UC-3 cells[J]. Journal of Shandong University (Health Sciences), 2018, 56(3): 41-47.

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