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山东大学学报 (医学版) ›› 2021, Vol. 59 ›› Issue (10): 32-40.doi: 10.6040/j.issn.1671-7554.0.2021.0725

• 临床医学 • 上一篇    下一篇

基于单细胞测序分析急性髓系白血病患者骨髓免疫微环境的特点

王艳1,张宇卉1,胡耐博1,滕广帅1,周圆2,白洁1   

  1. 1. 天津医科大学第二医院血液科, 天津 300211;2.中国医学科学院血液病医院 中国医学科学院血液学研究所 实验血液学国家重点实验室, 天津 300020
  • 发布日期:2021-10-15
  • 通讯作者: 白洁. E-mail:janebai86@hotmail.com
  • 基金资助:
    国家自然科学基金(81770128,81970120)

Characteristics of bone marrow immune microenvironment in patients with acute myeloid leukemia based on single-cell RNA sequencing

WANG Yan1, ZHANG Yuhui1, HU Naibo1, TENG Guangshuai1, ZHOU Yuan2, BAI Jie1   

  1. 1. Department of Hematology, The Second Hospital of Tianjin Medical University, Tianjin 300211, China;
    2. State Key Laboratory of Experimental Hematology, Institute of Hematology &
    Blood Diseases Hospital, National Clinical Research Center for Blood Diseases, Chinese Academy of Medical Sciences &
    Peking Union Medical College, Tianjin 300020, China
  • Published:2021-10-15

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摘要： 目的 通过单细胞测序技术分析急性髓系白血病(AML)患者骨髓免疫微环境的组成情况,探讨不同类型免疫细胞的不同亚群在AML发生发展中的作用。 方法 检索基因表达数据库(GEO)中符合条件的scRNA-seq数据集(GSE116256),利用R语言的Seurat包对数据进行处理,利用SingleR包并参考既往研究对细胞亚群进行注释,计算不同类型免疫细胞的比例,寻找AML患者与正常对照组存在差异的细胞亚群并计算它们的差异基因(DEGs),对DEGs进行基因本体功能注释(GO)和京都基因与基因组百科全书富集分析(KEGG),明确存在差异的细胞亚群在AML疾病进程中的作用。 结果 与正常对照组相比, AML患者的造血祖细胞、单核细胞、NK/T细胞、树突状细胞和B细胞的亚群比例均发生不同程度改变。通过对差异基因进行功能富集发现,AML的单核细胞、树突状细胞、B细胞以及T细胞的增殖、分化、免疫调控及对肿瘤细胞的杀伤功能出现不同程度损伤。 结论 AML患者与健康人的免疫微环境存在较大差异,这些差异可能与AML的发生发展关系密切。

关键词: 急性髓系白血病, 单细胞测序, 骨髓微环境, 免疫细胞, 生物信息

Abstract: Objective To analyze the composition of bone marrow immune microenvironment in patients with acute myeloid leukemia(AML)by single cell sequencing technology, and to explore the role of different subsets of different types of immune cells in the occurrence and development of AML. Methods The scRNA-seq data(GSE116256)which met the requirements of this study were downloaded from GEO database and processed with the Seurat package of R language. The cell subsets were annotated by single R package and reference to previous studies, the proportion of different types of immune cells was calculated, and the differentially expressed genes(DEGs)of cell subsets between AML patients and normal controls were calculated. Gene ontology function annotation(GO)and Kyoto Encyclopedia of genes and genomes(KEGG)enrichment analysis on DEGs were performed to clarify the role of cell subsets in the progression of AML. Results Compared with the normal control group, AML patients had changed proportion of progenitor cells, monocytes, NK/T cells, dendritic cells and B cells. GO analysis indicated that the proliferation, differentiation, immune regulation and killing function of monocytes, dendritic cells, B cells and T cells were damaged. Conclusion There are big differences in the immune microenvironment between AML patients and healthy people, and these differences may be closely related to the occurrence and development of AML.

Key words: Acute myeloid leukemia, Single cell sequencing, Bone marrow microenvironment, Immune cells, Bioinformatics

中图分类号: 

  • R551.3
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