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山东大学学报 (医学版) ›› 2021, Vol. 59 ›› Issue (4): 56-62.doi: 10.6040/j.issn.1671-7554.0.2020.1475

• 基础医学 • 上一篇    下一篇

室内照明白色发光二极管对大鼠视网膜的影响

南莉1,杨凯转2,张一帆1   

  1. 1. 天津市眼科医院 天津医科大学眼科临床学院 天津市眼科学与视觉科学重点实验室, 天津 300020;2. 郑州市第二人民医院眼科, 河南 郑州 450006
  • 发布日期:2021-04-30
  • 通讯作者: 南莉. E-mail:2460816277@qq.com
  • 基金资助:
    天津市自然科学基金(18JCZDJC36400)

Effects of domestic white light-emitting diodes exposure on rat retina

NAN Li1, YANG Kaizhuan2, ZHANG Yifan1   

  1. 1. Tianjin Eye Hospital, Clinical College of Ophthalmology, Tianjin Medical University, Tianjin Key Lab of Ophthalmology and Visual Science, Tianjin 300020, China;
    2. Department of Ophthalmology, Zhengzhou Second Hospital, Zhengzhou 450006, Henan, China
  • Published:2021-04-30

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摘要： 目的 比较不同照度白色LED光源对SD大鼠视网膜结构及功能的影响,以期获得较为安全的照度阈值。 方法 选取健康8~10周龄SD大鼠40只,随机分为正常对照组(8只)和实验组(4组,每组8只)。选取白色LED光源,以200、300、500、1 000 lux分别间歇性照射实验组动物,光照12 h,暗适应12 h,反复7次建立间歇性光损伤模型。造模后暗适应24 h,通过闪光视网膜电图(F-ERG)评估视网膜功能;苏木精-伊红(HE)染色和透射电子显微镜观察SD大鼠视网膜形态学改变;逆转录聚合酶链反应(RT-PCR)检测大鼠视网膜中Bax、Bcl-2及Caspese-3 mRNA的相对表达量。 结果 500、1 000 lux暗适应Rod-Rb、Max-Ra、Max-R、Ops波潜伏期及波幅下降。HE染色组织切片检查1 000 lux组视网膜损伤最严重;透射电子显微镜示500、1 000 lux细胞膜结构受损。实时荧光定量PCR显示500、1 000 lux照射后Bax和Caspase-3 mRNA相对表达量增加,Bcl-2 mRNA相对表达量减少。 结论 日常室内照明强度下,白色LED 7 d循环照射对SD大鼠视网膜结构和功能无显著影响,光源达到及超过500 lux时,可以引起SD大鼠视网膜光损伤,其损伤程度与照度有关。凋亡相关基因Bax、Caspase-3促进视网膜光损伤的发生与进展。

关键词: 白光, 二极管光源, 视网膜, 凋亡, 大鼠

Abstract: Objective To investigate the effects of different illumination levels of white light-emitting diodes(LED)light on the retinal structure and function of SD rats, in order to seek a relative safe illumination threshold. Methods A total of 40 rats aged 8-10 weeks were selected and randomly divided into control group and experimental groups(4 subgroups)with 8 animals in each group. After dark adaptation, the SD rats were exposed to 200, 300, 500 and 1 000 lux white LED light respectively in 12 hrs cycle for 7 days. After dark adaptation for 24 hours, the retinal function was evaluated with flash electroretinogram(F-ERG), and the histopathological changes were assessed with hematoxylin-eosin(HE)staining and transmission electron microscopy. Apoptotic cell death was evaluated with reverse transcription-polymerase chain reaction(RT-PCR)via the relative mRNA expressions of Bax, Bcl-2 and Caspase-3. Results Scotopic ERG showed that 500 and 1 000 lux white LED exposure resulted in a decrease in Rod-Rb, Max-Ra, Max-Rb and Ops. In the 1 000 lux group, HE staining revealed massive retinal damage. In the 500 and 1 000 lux groups, transmission electron microscopy showed cell membrane structure damage. RT-PCR showed the relative mRNA expressions of Bax and Caspase-3 increased and the mRNA expression of Bcl-2 decreased after 500 and 1 000 lux irradiation. Conclusion Under daily indoor lighting intensity, 7-day cycle of white LED irradiation has no significant effects on the retinal structure and function of SD rats. Light intensity up to 500 lux can cause retinal photodamage in SD rats in a dose-dependent manner. The apoptosis-related genes Bax and Caspase-3 promote the occurrence and progression of retinal light damage.

Key words: White light, Light-emitting diodes, Retina, Apoptosis, SD rat

中图分类号: 

  • R774.1+3
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