白头翁皂苷D联合索拉非尼对人肝癌细胞侵袭与转移的影响

doi:10.6040/j.issn.1671-7554.0.2015.610

摘要/Abstract

摘要： 目的探讨白头翁皂苷D联合索拉非尼对人肝癌细胞株侵袭和转移的影响。方法以人肝癌细胞株SMMC-7721细胞为研究对象,分为白头翁皂苷D组(浓度为11.9 μg /L)、索拉非尼组(浓度为2.15 μmol/L)、联合组(白头翁皂苷D 11.9 μg/L+索拉非尼2.15 μmol/L)以及对照组(普通培养液)。通过MTT法、Transwell小室实验观察白头翁皂苷D、索拉非尼单药和联合对SMMC-7721细胞的侵袭和转移的抑制作用,并用免疫组织化学方法检测CD_44V6、血管内皮生长因子C(VEGF-C)、基质金属蛋白酶MMP-9基因蛋白表达的影响。结果 MTT结果显示,白头翁皂苷D、索拉非尼单药和联合对SMMC-7721细胞的增殖均有抑制作用,且24 h的抑制率都<15%;Transwell小室实验结果显示,联合组的黏附抑制率与单药组相比,具有协同抑制作用,但未呈现时间依赖关系;联合组的侵袭抑制率和迁移抑制率均显著高于单药组(P<0.01);免疫组织化学结果表明,联合组在下调CD_44V6、VEGF-C、MMP-9方面均高于单药组(P<0.05)。结论白头翁皂苷D和索拉非尼联合可协同抑制SMMC-7721细胞侵袭和转移,联合效果整体优于单药。

关键词: 白头翁皂苷D, 侵袭, 转移, 肝癌细胞, 索拉非尼

Abstract: Objective To investigate the effect of Pulsatilla saponin D and sorafenib on the invasion and metastasis of human hepatoma cell line. Methods The human hepatoma cell line SMMC-7721 cells were divided into Pulsatilla saponin D group(11.9 μg/L), sorafenib group(2.15 μmol/L), combined group(Pulsatilla saponin D 11.9 μg/L+Sorafenib 2.15 μmol/L)and control group(ordinary culture). The inhibitory effects on the migration and invasion of SMMC-7721 cells were observed with MTT assay and Transwell chamber experiments. The effects on CD_44V6, VEGF-C, MMP-9 gene protein expressions were detected with immunocytochemistry. Results MTT assay showed that Pulsatilla saponin D, sorafenib, and combined therapy inhibited the proliferation of SMMC-7721 cells, and the 24 h inhibition rate was less than 15%. Transwell chamber experiments showed that the adhesion inhibition rate of the combined group displayed a synergistic effect compared with the monotherapy groups, but not in a time-dependent manner; the invasion and migration inhibition rate of the combined group were significantly higher than those of the monotherapy groups(P<0.01). Immunohistochemistry results showed that the combined group had lower CD_44V6, VEGF-C, and MMP-9 expressions than the monotherapy groups(P<0.05). Conclusion Pulsatilla saponin D and sorafenib synergistically inhibit the SMMC-7721 cell invasion and metastasis, and the combined effect of Pulsatilla saponin D and sorafenib is 山东大学学报 (医学版)54卷7期 -贺武斌,等.白头翁皂苷D联合索拉非尼对人肝癌细胞侵袭与转移的影响 \=-better than the single drug.

Key words: Pulsatilla saponin D, Sorafenib, Invasion, Hepatoma cells, Metastasis

中图分类号:

R735.7

贺武斌,苏荣健. 白头翁皂苷D联合索拉非尼对人肝癌细胞侵袭与转移的影响[J]. 山东大学学报（医学版）, 2016, 54(7): 18-22.

HE Wubin, SU Rongjian. Pulsatilla saponin D combined with sorafenib against hepatocellular carcinoma cell invasion and metastasis[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(7): 18-22.

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