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山东大学学报（医学版） ›› 2017, Vol. 55 ›› Issue (10): 41-45.doi: 10.6040/j.issn.1671-7554.0.2016.1522

• 基础医学 • 上一篇    下一篇

CD11b+/CD66b-表型髓系细胞在结肠癌进展和肝转移中的作用

王贲士1, 单军奇1,2,侯庆生1,公为鹏1,朱振宇1,郭洪亮1   

  1. 1.山东省肿瘤医院外科四病区, 山东 济南 250117;2.济南大学 山东医学科学院与生命科学学院, 山东 济南 250022
  • 收稿日期:2016-11-17 出版日期:2017-10-10 发布日期:2017-10-10
  • 通讯作者: 郭洪亮. E-mail: hliangbb@163.com E-mail:hliangbb@163.com
  • 基金资助:
    山东省自然科学基金(ZR2013HL045)

Effort of CD11b+ / CD66b- phenotype myeloid cells on the progression of colon cancer and liver metastasis

WANG Bishi1, SHAN Junqi1,2, HOU Qingsheng1, GONG Weipeng1, ZHU Zhenyu1, GUO Hongliang1   

  1. 1. The fourth department of surgical ward, Shandong Cancer Hospital, Jinan 250117, Shandong, China;
    2.School of Medicine and Life Science, University of Jinan-Shandong Academy of Medical Sciences, Jinan 250022, Shandong, China
  • Received:2016-11-17 Online:2017-10-10 Published:2017-10-10

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摘要： 目的 探讨CD11b+/CD66b-表型髓系细胞与人类结肠癌进展和肝转移的关系。 方法 采用real-time PCR、Western blotting及免疫组化方法检测正常结肠组织、结肠癌组织及结肠癌肝转移组织中CD11b、趋化因子配体2(CCL2)和趋化因子受体2(CCR2)的转录水平、蛋白翻译水平。免疫共沉淀方法检测CCL2和CCR2间的相互结合关系。 结果 结肠癌组织、肝转移瘤组织的CD11b mRNA转录水平及蛋白的表达水平较癌旁正常组织高(P<0.05)。肝转移瘤组织中CCL2 的mRNA转录水平及蛋白的表达水平明显高于癌旁正常结肠组织及结肠癌组织(P<0.05),而CCR2mRNA转录水平及蛋白表达水平在三者中无统计学差异(P>0.05)。趋化因子配体CCL2和趋化因子受体CCR2之间可相互结合。 结论 CD11b表型髓系细胞在结肠癌组织及结肠癌肝转移组织中广泛浸润,并可能在CCL2-CCR2轴的介导下促进结肠癌进展和结肠癌肝转移。

关键词: 结肠癌, 肝转移, CD11b+/CD66b-表型髓系细胞, 趋化因子受体2, 趋化因子配体2

Abstract: Objective To study the relationship between CD11b+/ CD66b- phenotype myeloid cells and colon cancer progression and liver metastasis. Methods The transcription level, protein translation levels of CD11b, chemokine C-C motif ligand 2(CCL2)and chemokine C-C motif receptor type 2(CCR2)in colonic carcinoma tissues, colorectal cancer tissues and liver metastasis were detected by real-time PCR, Western blotting and immunohistochemistry. The co-immunoprecipitation method was used to detect the relationship between CCL2 and CCR2. Results The expression of CD11b mRNA in colorectal carcinoma and liver metastasis was higher than that in normal tissues(P<0.05). The level of CCL2 mRNA transcription and protein expression in liver metastasis was significantly higher than that in normal colonic tissues and colon cancer tissues(P<0.05). The level of CCR2 mRNA transcription and protein expression in the three were no significant difference(P>0.05). Chemokine ligand CCL2 and chemokine receptor CCR2 can be combined with each other. Conclusion CD11b phenotype myeloid cell is extensive infiltration in colon cancer and liver metastasis of colon cancer tissue, and possibly can promote the progression of colon cancer and liver metastasis of colonic carcinoma by mediating with CCL2-CCR2 axis.

Key words: Liver metastasis, Chemokine C-C motif receptor type 2, Colorectal cancer, CD11b+/CD66b-Myeloid cells, Chemokine C-C motif ligand 2

中图分类号: 

  • R735.3+5
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