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山东大学学报（医学版） ›› 2014, Vol. 52 ›› Issue (8): 57-62.doi: 10.6040/j.issn.1671-7554.0.2013.773

• 临床医学 • 上一篇    下一篇

循环miR-128在结直肠癌患者血清中的表达及其对细胞迁移侵袭能力的影响

张艳丽1, 刘新风1, 张欣2, 王海燕2, 杨咏梅2, 杜鲁涛2, 王丽丽2, 李培龙2, 王传新2   

  1. 1. 山东省交通医院检验科, 山东 济南 250031;
    2. 山东大学齐鲁医院检验科, 山东 济南 250012
  • 收稿日期:2013-12-27 修回日期:2014-07-02 出版日期:2014-08-10 发布日期:2014-08-10
  • 通讯作者: 王传新。E-mail：wcx6601@126.com E-mail:wcx6601@126.com
  • 基金资助:
    国家自然科学基金（81271916，81301506）；高等学校博士学科点专项科研基金（20130131120067）；山东省自然科学基金（ZR2013HQ063）

Expression of circulating miR-128 in serum of colorectal cancer and its effect on migration and invasion of colorectal cancer cells

ZHANG Yanli1, LIU Xinfeng1, ZHANG Xin2, WANG Haiyan2, YANG Yongmei2, DU Lutao2, WANG Lili2, LI Peilong2, WANG Chuanxin2   

  1. 1. Department of Clinical Laboratory, Traffic Hospital of Shandong Province, Jinan 250031, Shandong, China;
    2. Department of Clinical Laboratory, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2013-12-27 Revised:2014-07-02 Online:2014-08-10 Published:2014-08-10

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摘要： 目的 检测循环miR-128在结直肠癌患者血清中的表达，观察其对结直肠癌细胞迁移侵袭能力的影响。 方法 实时荧光定量PCR检测57例结直肠癌组和20例健康对照组血清中miR-128的表达，分析其与结直肠癌转移的关系。体外实验观察过表达miR-128对HT-29细胞迁移侵袭能力的影响。结果 结直肠癌组血清miR-128水平明显低于健康对照组（P<0.001），其中转移组水平明显低于未转移组（P=0.014）。ROC曲线分析显示，血清miR-128对诊断结直肠癌和转移鉴别诊断的效能分别为0.85、0.71（P均<0.05）。与阴性对照组和空白对照组比较，转染组的细胞迁移愈合率以及迁移、侵袭细胞数明显减少（P均<0.05）。结论 循环miR-128在结直肠癌患者血清中表达明显下调，并且与转移密切相关，为结直肠癌转移诊断和治疗提供了新思路。

关键词: 结直肠癌, MiR-128, 转移, 侵袭, 迁移

Abstract: Objective To detect the expression of miR-128 in the serum of patients with colorectal cancer and to observe its effect on the migration and invasion of colorectal cancer cells. Methods The serum miR-128 levels were detected by quantitative real-time PCR in 57 cases of colorectal cancer and 20 healthy controls. The correlation between miR-182 expression and colorectal cancer metastasis was analyzed. The migration and invasion ability were observed by vitro experiments in HT-29 cells transfected with miR-128 mimics. Results The miR-128 expression in colorectal cancer was significantly lower than that in healthy controls (P<0.001), and it markedly decreased in the colorectal cancer metastasis group compared with non-metastasis group (P=0.014). The diagnosis and metastasis differential diagnosis capability of serum miR-128 for colorectal cancer was 0.85 and 0.71 (both P<0.05). Compared with the negative control group and blank control group, the migration healing rates and number of migration and invasion cells in the transfection group were significantly decreased (all P<0.05). Conclusion Circulating miR-128 is significantly decreased in serum of colorectal cancer and closely associated with colorectal cancer metastasis, which may provide a new idea for the diagnosis and therapy of colorectal cancer.

Key words: Metastasis, Invasion, Migration, Colorectal cancer, MiR-128

中图分类号: 

  • R735.3+4
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