周围神经吻合后NGFFG胶膜包埋的实验研究

山东大学学报(医学版) ›› 2009, Vol. 47 ›› Issue (9): 28-31.

周围神经吻合后NGFFG胶膜包埋的实验研究

高春正，马胜忠，王集锷

山东大学第二医院骨科，济南 250033

收稿日期:2009-06-09 发布日期:2009-09-16
通讯作者: 马胜忠，博士后，硕士生导师，主要从事脊柱外科学的研究。 Email：mszfirst@yahoo.com.cn
作者简介:高春正（1964- ），男，医学博士，硕士生导师，主要从事脊柱与周围神经损伤的研究。Email:gaochunzheng@yahoo.com.cn

Application of nerve growth factors and fibral glue after 
anastomosis of peripheral nerves in mice

GAO Chunzheng， MA Shengzhong， WANG Jie

Department of Orthopaedics, Second Hospital of Shandong University, Jinan 250033, China

Received:2009-06-09 Published:2009-09-16

摘要/Abstract

摘要：

目的观察小鼠坐骨神经离断后，神经生长因子-纤维蛋白胶(NGFFG)混合胶膜吻合口包埋对坐骨神经再生的促进作用。方法随机将昆明小白鼠40只分为吻合包埋组(实验组)和单纯吻合组(对照组)，实验组外膜吻合后加用NGFFG胶膜包埋，对照组单纯外膜缝合。分别于术后4、8、12周，检测坐骨神经功能指数（SFI）、动作电位的振幅（NAP）及神经传导速度(NCV)；光镜观察再生神经纤维数量、通过率和纤维组织侵入情况；电镜观察神经纤维及轴突的直径、结构和髓鞘厚度。结果术后4、8、12周不同时间点实验组的NAP及NCV均大于对照组（P＜0.05）,术后4周两组动物SFI均开始恢复，随时间的推移恢复率逐渐提高，实验组恢复情况优于对照组（P＜0.05）。光镜下单位面积吻合口内再生神经纤维数量实验组明显多于对照组，电镜下两组再生神经纤维、轴突直径及髓鞘厚度差异不明显，均低于正常神经纤维，实验组再生神经纤维的微观结构较对照组清晰，成熟度好于对照组。结论纤维蛋白胶可以作为外源性神经生长因子的有效载体，用含有神经生长因子的纤维蛋白胶包埋离断周围神经的吻合口，能促进神经纤维的再生并有效防止吻合口与周围组织的粘连，减少纤维组织的侵入、神经纤维瘤的形成。

关键词: 周围神经, 再生, 纤维蛋白组织粘合剂, 神经甥长因子, 小鼠

Abstract:

To study the effects of fibrin sealant containing nerve growth factor on peripheral nerve regeneration. Methods40 Kunming mice were randomly divided into two groups: in the processing group the stoma of the nerve was coated by glue membrane mixed with fiberal glue and nerve growth factors （NGFFG）and in the control group only endtoend anastomosis was performed with a 100 suture silk. At 4, 8 and 12?w after the operations, sciatic nerve function index（SFI）, peak of action potential of triceps surae（NAP）and the conduct speed of the sciatic nerve (NCV) were determined. Under microscopy the number of regenerated nerve fibers per view in the stoma was counted and the invasions of fibrous tissues into the stoma were observed. Under electron microscopy, diameter of regenerated nerve fibers and their axon and thickness of the myelin sheath were measured. ResultsIn each observation stage the sciatic nerve function index, conduct speed of the sciatic nerve and peak of action potential of triceps surae of the processing group were better than those of the control group（P＜0.05）. Under microscopy, the number of regenerated nerve fibers per view in the stoma was more in the processing group than in the control group（P＜0.05）. The incident rate of invasion of fibrous tissues into the stoma in the processing group was much lower than that in the control group. Under electron microscopy, diameter of regenerated nerve fibers and their axon and thickness of the myelin sheath did not vary between the two groups but were both lower than those of normal nerves, however the maturation degree in the processing group was better than that in the control group. ConclusionsNerve growth factors and fiberal glue used after anastomosis of peripheral nerves prevent invasion of fibrous tissues into the stoma, adhere between the stoma and its surrounding tissues, and effectively step up the growth and regeneration of nerve fibers.

Key words: Peripheral nerves; Regeneration; Fibrin tissue adhes ive; Nerve growth factor; Mice

中图分类号:

R745

. 周围神经吻合后NGFFG胶膜包埋的实验研究[J]. 山东大学学报(医学版), 2009, 47(9): 28-31.

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