醛化白及多糖/羟丙基壳聚糖/纳米珍珠层粉复合材料促下颌骨缺损修复的动物实验研究

doi:10.6040/j.issn.1671-7554.0.2015.341

山东大学学报（医学版） ›› 2016, Vol. 54 ›› Issue (6): 7-11.doi: 10.6040/j.issn.1671-7554.0.2015.341

醛化白及多糖/羟丙基壳聚糖/纳米珍珠层粉复合材料促下颌骨缺损修复的动物实验研究

陈怡憓¹,刘学蔚¹,侯绪浩²,孙钦峰¹

1. 山东大学口腔医学院牙周科山东省口腔生物医学重点实验室, 山东济南 250012;2. 山东中医药大学药学院, 山东济南 250355

收稿日期:2015-04-01 出版日期:2016-06-20 发布日期:2016-06-20
通讯作者: 孙钦峰. E-mail:sunqinfeng@sdu.edu.cn E-mail:sunqinfeng@sdu.edu.cn
基金资助:
山东省科技攻关计划(2014GGE27400)

Study of mouse alveolar bone defect reparation by dialdehyde bletilla striata glucomanna / hydroxypropyl chitosan / nano-nacre powder composited scaffolds

CHEN Yihui¹, LIU Xuewei¹, HOU Xuhao², SUN Qinfeng¹

1. Department of Periodontics, School of Stomatology, Shandong University, Shandong Provincial Key Laboratory of Oral Biomedicine, Jinan 250012, Shandong, China;
2. School of Pharmacy, Shandong University of Chinese Traditional Medicine, Jinan 250355, Shandong, China

Received:2015-04-01 Online:2016-06-20 Published:2016-06-20

摘要/Abstract

摘要： 目的研究醛化白及多糖(DBsGM)/羟丙基壳聚糖(HPCS)/纳米珍珠层粉(NNP)复合支架材料对大鼠下颌骨缺损愈合过程的成骨促进作用,并观察该材料的降解吸收情况。方法取32只雄性Wistar大鼠,于每只大鼠双侧下颌磨牙区作箱型骨缺损,根据处死时间(2、4、6、8周)随机分成4组,每组8只,在4组中实验侧植入DBsGM/HPCS/NNP复合支架材料,对照侧不植入任何材料。切片经苏木精-伊红染色,组织学测量评价骨缺损修复效果。结果两组新生骨面积百分比随愈合时间延长逐渐增加(P<0.05);相同愈合时间的实验侧新生骨面积优于对照侧(P<0.05)。植入材料随着时间逐渐降解,第6~8周,植入材料基本降解完全。结论 DBsGM/HPCS/NNP复合支架材料在大鼠下颌骨缺损中具有良好的促进成骨作用。

关键词: 牙周组织再生, 组织工程, 大鼠骨缺损, 纳米珍珠层粉, 白及胶

Abstract: Objective To observe the osteogenesis and degradation of dialdehyde letilla striata glucomanna(DBsGM)/hydroxypropyl chitosan(HPCS)/nano-nacre powder(NNP)composited scaffolds in rat alveolar bone defect regeneration. Methods A total of 32 male Wistar rats with bilateral mandibular molar area box defects were randomly divided into 4 groups according to the disposal time(week 2, 4, 6, 8), with 8 animals in each group. The DBsGM/HPCS/NNP composited scaffolds were implanted in the experimental side, while nothing was implanted in the control side. The bone defect repair effect was evaluated with hematoxylin-eosin staining and histologic measurement. Results As time extended, the percentage of new bone area increased gradually, and there was significant difference between different time points(P<0.05). At the same time point, the experimental group was superior to the control group in gross observation and histological observation(P<0.05). The implanted materials gradually degraded with time. During week 6 to 8, the implanted materials completely degraded. Conclusion DBsGM/HPCS/NNP composited scaffolds can promote rat alveolar bone defect repair and regeneration.

Key words: Tissue engineering, Periodontal tissue regeneration, Nano-nacre powder, Bletilla striata glucomannan, Periodontal defects in rats

中图分类号:

R781

陈怡憓,刘学蔚,侯绪浩,孙钦峰. 醛化白及多糖/羟丙基壳聚糖/纳米珍珠层粉复合材料促下颌骨缺损修复的动物实验研究[J]. 山东大学学报（医学版）, 2016, 54(6): 7-11.

CHEN Yihui, LIU Xuewei, HOU Xuhao, SUN Qinfeng. Study of mouse alveolar bone defect reparation by dialdehyde bletilla striata glucomanna / hydroxypropyl chitosan / nano-nacre powder composited scaffolds[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(6): 7-11.

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醛化白及多糖/羟丙基壳聚糖/纳米珍珠层粉复合材料促下颌骨缺损修复的动物实验研究

Study of mouse alveolar bone defect reparation by dialdehyde bletilla striata glucomanna / hydroxypropyl chitosan / nano-nacre powder composited scaffolds

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