细胞角蛋白14基因在非小细胞肺癌中的表达与意义

doi:10.6040/j.issn.1671-7554.0.2016.781

摘要/Abstract

摘要： 目的探讨细胞角蛋白14(CK14)基因的表达在非小细胞肺癌(NSCLC)发生、发展及转移中的意义。方法采用免疫组织化学染色法检测110例NSCLC患者癌组织、癌旁组织、正常肺组织中CK14的表达。采用Western blotting法检测12例患者新鲜组织中CK14的表达。应用非条件Logistic回归分析CK14表达水平与NSCLC患者各临床病理参数的关系。结果免疫组织化学染色结果显示,CK14在NSCLC癌组织中高表达,阳性表达率为68.18%,与癌旁组织和正常肺组织相比,差异有统计学意义(P<0.001)。CK14表达水平与NSCLC患者性别、组织类型、分化程度、分期以及淋巴结转移情况显著相关(P<0.05),与年龄无关(P>0.05)。Western blotting结果显示,CK14在NSCLC癌组织中的表达率较癌旁组织和正常肺组织高。非条件Logistic分析显示,低分化癌、病理分期Ⅱ期和Ⅲ期、CK14表达阳性是NSCLC患者发生淋巴结转移的重要危险因素(P<0.05)。结论 CK14表达在NSCLC的发生、发展和转移中发挥重要作用。CK14有望成为评估NSCLC生物学行为的重要指标。

关键词: 癌, 非小细胞肺, Logistic回归分析, 免疫组织化学, 细胞角蛋白14

Abstract: Objective To investigate the expression of CK14 and its significance in the occurrence, development and metastasis of non-small cell lung cancer(NSCLC). Methods A total of 110 NSCLC patients were involved in the study. The NSCLC cancerous tissues, the tissues adjacent to carcinoma and normal lung tissues of 110 patients and 12 patients were analyzed for CK14 expression by immunohistochemistry and Western blotting, respectively. The relationship between CK14 expression and clinical pathological parameters of NSCLC patients was analyzed by non-conditional Logistic regression analysis. Results The immunohistochemistry results showed that CK14 was overexpressed in NSCLC cancerous tissues which was significantly higher than tissues adjacent to carcinoma and normal lung tissues(P<0.001). In addition, the positive rate of CK14 was 68.18%. Its level was significantly correlated with sex, histopathologic type, differentiation of tumors, clinical stages and lymph node metastasis(P<0.05). However, no correlation was observed between CK14 level and age(P>0.05). Additionally, Western blotting result also showed that CK14 was overexpressed in NSCLC cancerous tissues compared with the tissues adjacent to carcinoma and normal lung tissues. The results of non-conditional Logistic regression analysis showed that poor differentation, staging Ⅱ and Ⅲ, and the positive expression of CK14 were important risk factors in lymph node metastasis of NSCLC(P<0.05). Conclusion CK14 plays a promotional role in the development and metastatic progression of NSCLC. CK14 expression would be a significant biomarker in evaluating the biological malignant behavior of NSCLC.

Key words: Carcinoma, non-small-cell lung, Cytokeratin 14, Logistic regression analysis, Immunohistochemistry

中图分类号:

R734.2

王永强,张维全,孙启峰,董晓鹏,彭传亮,张媛,赵小刚. 细胞角蛋白14基因在非小细胞肺癌中的表达与意义[J]. 山东大学学报（医学版）, 2017, 55(3): 83-87.

WANG Yongqiang, ZHANG Weiquan, SUN Qifeng, DONG Xiaopeng, PENG Chuanliang, ZHANG Yuan, ZHAO Xiaogang. Expression and significance of CK14 in non-small cell lung cancer[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(3): 83-87.

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