山东大学学报 (医学版) ›› 2024, Vol. 62 ›› Issue (6): 30-37.doi: 10.6040/j.issn.1671-7554.0.2024.0261
• 基础医学 • 上一篇
王潇1,孔文茹1,崔伟亮2,王姝麒1
WANG Xiao1, KONG Wenru1, CUI Weiliang2, WANG Shuqi1
摘要: 目的 观察羊角棉总生物碱在结直肠癌治疗中对氟尿嘧啶(5-fluorouracil, 5-FU)的增敏作用,探讨其对5-FU药代动力学的影响。 方法 CCK-8法评价羊角棉总生物碱与5-FU联用对HCT15人结直肠腺癌细胞和CT26.WT小鼠结肠癌细胞杀伤能力;构建小鼠皮下瘤模型,观察5-FU和羊角棉总生物碱单用或联用时对肿瘤生长的影响;LC-MS/MS法检测5-FU及其代谢物二氢氟尿嘧啶(dihydrofluorouracil, FUH2)和氟脱氧尿苷(fluorodeoxyuridine, FUrd)在大鼠体内的药代动力学参数。 结果 羊角棉总生物碱对HCT15和CT26.WT细胞毒性的IC50值分别为31.98 μg/mL和23.71 μg/mL;低剂量(100 mg/kg)和高剂量(200 mg/kg)羊角棉总生物碱联用5-FU对荷瘤小鼠的抑瘤率从50.91%分别增长至58.56%和62.16%;5-FU联用羊角棉总生物碱后,T1/2显著延长,其代谢产物FUrd血药浓度显著下降。 结论 羊角棉总生物碱对结直肠癌细胞有一定的细胞毒性,与5-FU联用可显著增强其抗结直肠癌活性;羊角棉总生物碱可能通过降低二氢尿嘧啶脱氢酶浓度延缓5-FU分解代谢并调节其合成代谢途径,从而加强其生物学活性。
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[1] Bray F, Ferlay J, Soerjomataram I, et al. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J]. CA Cancer J Clin, 2018, 68(6): 394-424. [2] Dekker E, Tanis PJ, Vleugels JLA, et al. Colorectal cancer[J]. Lancet, 2019, 394(10207): 1467-1480. [3] Arnold M, Sierra MS, Laversanne M, et al. Global patterns and trends in colorectal cancer incidence and mortality[J]. Gut, 2017, 66(4): 683-691. [4] Siegel RL, Fedewa SA, Anderson WF, et al. Colorectal cancer incidence patterns in the United States, 1974-2013[J]. J Natl Cancer Inst, 2017, 109(8): djw322. doi:10.1093/jnci/djw322. [5] Bailey CE, Hu CY, You YN, et al. Increasing disparities in the age-related incidences of colon and rectal cancers in the United States, 1975-2010[J]. JAMA Surg, 2015, 150(1): 17-22. [6] Wolf AMD, Fontham ETH, Church TR, et al. Colorectal cancer screening for average-risk adults: 2018 guideline update from the American Cancer Society[J]. CA Cancer J Clin, 2018, 68(4): 250-281. [7] Kasi PM, Shahjehan F, Cochuyt JJ, et al. Rising proportion of young individuals with rectal and colon cancer[J]. Clin Colorectal Cancer, 2019, 18(1): e87-e95. [8] Glynne-Jones R, Wyrwicz L, Tiret E, et al. Rectal cancer: ESMO Clinical Practice Guidelines for diagnosis, treatment and follow-up[J]. Ann Oncol, 2018, 29(Suppl 4): iv263. doi:10.1093/annonc/mdy161. [9] Vodenkova S, Buchler T, Cervena K, et al. 5-Fluorouracil and other fluoropyrimidines in colorectal cancer: past, present and future[J]. Pharmacol Ther, 2020, 206: 107447. doi:10.1016/j.pharmthera.2019.107447. [10] Veenstra CM, Krauss JC. Emerging systemic therapies for colorectal cancer[J]. Clin Colon Rectal Surg, 2018, 31(3): 179-191. [11] Blondy S, David V, Verdier M, et al. 5-Fluorouracil resistance mechanisms in colorectal cancer: from classical pathways to promising processes[J]. Cancer Sci, 2020, 111(9): 3142-3154. [12] Heidelberger C, Chaudhuri NK, Danneberg P, et al. Fluorinated pyrimidines, a new class of tumour-inhibitory compounds[J]. Nature, 1957, 179(4561): 663-666. [13] Sargent D, Sobrero A, Grothey A, et al. Evidence for cure by adjuvant therapy in colon cancer: observations based on individual patient data from 20, 898 patients on 18 randomized trials[J]. J Clin Oncol, 2009, 27(6): 872-877. [14] Latchman J, Guastella A, Tofthagen C. 5-Fluorouracil toxicity and dihydropyrimidine dehydrogenase enzyme: implications for practice[J]. Clin J Oncol Nurs, 2014, 18(5): 581-585. [15] Zhang LL, Xing XL, Meng FL, et al. Oral fluoropyrimidine versus intravenous 5-fluorouracil for the treatment of advanced gastric and colorectal cancer: Meta-analysis[J]. J Gastroenterol Hepatol, 2018, 33(1): 209-225. [16] Ahn DH, Wu C, Wei L, et al. The efficacy of adjuvant chemotherapy in patients with stage II/III resected rectal cancer treated with neoadjuvant chemoradiation therapy[J]. Am J Clin Oncol, 2017, 40(6): 531-534. [17] Lee GY, Lee JS, Son CG, et al. Combating drug resistance in colorectal cancer using herbal medicines[J]. Chin J Integr Med, 2021, 27(7): 551-560. [18] Li YQ, Cai J, Cheng JX, et al. Alstomairines D-G: new monoterpenoid indole alkaloids with cytotoxic activity from Alstonia mairei[J]. J Mol Struct, 2022, 1253: 132277. doi:10.1016/j.molstruc.2021.132277. [19] Yan TL, Han DX, Hu J, et al. Monoterpenoid indole alkaloids from Alstonia mairei and their cytotoxicity[J]. J Asian Nat Prod Res, 2017, 19(6): 550-556. [20] Cai XH, Zeng CX, Feng T, et al. Monoterpenoid indole alkaloids from Alstonia mairei[J]. Helv Chim Acta, 2010, 93(10): 2037-2044. [21] Sobrero A, Aschele C, Guglielmi A, et al. Resistance to 5-fluorouracil and 5-fluoro-2'-deoxyuridine mechanisms and clinical implications[J]. J Chemother, 1990, 2(Suppl 1): 12-16. [22] Johnston PG, Kaye S. Capecitabine: a novel agent for the treatment of solid tumors[J]. Anticancer Drugs, 2001, 12(8): 639-646. [23] Longley DB, Harkin DP, Johnston PG. 5-Fluorouracil: mechanisms of action and clinical strategies[J]. Nat Rev Cancer, 2003, 3(5): 330-338. [24] Diasio RB, Harris BE. Clinical pharmacology of 5-fluorouracil[J]. Clin Pharmacokinet, 1989, 16(4): 215-237. |
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