您的位置:山东大学 -> 科技期刊社 -> 《山东大学学报(医学版)》

山东大学学报 (医学版) ›› 2023, Vol. 61 ›› Issue (4): 10-17.doi: 10.6040/j.issn.1671-7554.0.2022.0705

• 基础医学 • 上一篇    

不同炖煮温度燕窝次生代谢化学成分组学

王智璠1,林小仙2,3,阴佳璐2,3,王东亮2,3,王姝麒1   

  1. 1.山东大学药学院, 山东 济南 250012;2.北京小仙炖生物科技有限公司, 北京 100020;3.河北省燕窝鲜炖技术创新中心, 河北 廊坊 065700
  • 发布日期:2023-04-11
  • 通讯作者: 王东亮. E-mail:dongliang.wang@xxdun.com王姝麒. E-mail:wangsq@sdu.edu.cn
  • 基金资助:
    山东省重点研发计划重大科技创新工程项目(2021CXGC010511);山东省重点研发计划(2017GSF218049);山东大学青年学者未来计划(2018WLJH93)

Secondary metabolites of edible birds nest prepared by different stewing temperatures

WANG Zhifan</sup>1, LIN Xiaoxian2,3, YIN Jialu2,3, WANG Dongliang2,3, WANG Shuqi1   

  1. 1. School of Pharmaceutical Sciences, Shandong University, Jinan 250012, Shandong, China;
    2. Beijing Xiaoxiandun Stew Biotechnology Co., Ltd., Beijing 100020, China;
    3. Hebei Edible Birds Nest Fresh Stew Technology Innovation Center, Langfang 065700, Hebei, China
  • Published:2023-04-11

摘要: 目的 基于LC-MS的代谢组学方法研究不同炖煮温度燕窝制品的次生代谢化合物差异性标志物。 方法 采用UPLC-MS技术, 结合多元统计分析方法, 以燕窝及其不同炖煮温度的燕窝制品为实验材料, 对其次生代谢产物进行化学成分差异性分析,筛查其差异性的特征化合物。 结果 一级正离子和负离子模式质谱共检测到10 687个离子,Compound Discoverer 3.0软件理论数据库鉴定了燕窝成品中30个化学成分,其中有10余个具有活性的燕窝成分。主成分分析(PCA)结果表明, 燕窝成品中次生代谢化合物在炖煮温度不同时未见明显差异,因此采用正交偏最小二乘法判别分析(OPLS-DA)方法筛查高温炖煮组和低温炖煮组之间的差异化合物, 结果显示6种化合物含量存在差异, 部分化合物经高温炖煮后含量减少, 在低温炖煮时含量升高(P<0.05)。 结论 6种化合物可以作为不同炖煮方法的差异化合物, 可用于指导燕窝制品炖煮程度的过程控制。

关键词: 燕窝, 炖煮方法, 代谢组学, 主成分分析, 代谢物鉴定, 差异化学成分

Abstract: Objective To investigate the differential markers of secondary metabolites in edible birds nest products at different stewing temperatures. Methods Edible birds nest and edible birds nest products prepared at different temperatures were analyzed with UPLC-MS technology and multivariate statistical analysis to assess the chemical composition differences of secondary metabolites and to screen the characteristic compounds of the differences. Results Primary positive and negative ion mode mass spectrometry detected 10,687 ions, and the Compound Discoverer 3.0 software identified 30 chemical components in finished products, including more than 10 active components. The results of principal component analysis(PCA)showed that there was little difference in secondary metabolites in finished edible birds nest products with different processing temperature. Therefore, the orthogonal partial least squares discriminant analysis(OPLS-DA)method was used to screen the differential compounds between the high and low temperature stewing groups. The results showed that the contents of 6 compounds were different, and the contents of some compounds decreased after high temperature stewing, but increased during low temperature stewing(P<0.05). Conclusion The 6 compounds can be used as differential compounds in different stewing methods, and can be used to guide the process control of stewing degree of edible birds nest products.

Key words: Edible birds nest, Stewing methods, Metabonomics, Principal component analysis, Metabolites identification, Differential chemical composition

中图分类号: 

  • R574
[1] 胡雅妮, 李峰, 康廷国. 燕窝的研究进展[J]. 中国中药杂志, 2003, 28(11): 1003-1005.
[2] 陈玲, 陈昕露, 范群艳, 等. 燕窝主要营养成分及其唾液酸酶法提取工艺 [J]. 福建农林大学学报(自然科学版), 2015, 44(5): 542-547. CHEN Ling, CHEN Xinlu, FAN Qunyan, et al. Major nutrients of edible birds nest and enzymatic extraction of sialic acid[J]. Journal of Fujian Agriculture and Forestry University, 2015, 44(5): 542-547.
[3] 简叶叶, 李庆旺, 黄灿灿, 等. 干燕窝与炖煮燕窝的营养成分分析与比较[J]. 福建轻纺, 2016(3): 32-38. doi:10.3969/j.issn.1007-550X.2016.03.001.
[4] 范小龙, 陈冉, 吴婉琴, 等. 液相色谱串联质谱法测定食品中淫羊藿苷等20种功能成分[J]. 食品与机械, 2021, 37(1): 86-93, 98. FAN Xiaolong, CHEN Ran, WU Wanqin, et al. Determination of icariin and other 20 functional components in food by liquid chromatography tandem mass spectrometry[J]. Food & Machinery, 2021, 37(1): 86-93, 98.
[5] 冯峰, 杨烁, 凌云, 等. 超高效液相色谱-串联质谱快速筛查葡萄酒中的14种禁用食品添加剂[J]. 分析化学, 2011, 39(11): 1732-1737. FENG Feng, YANG Shuo, LING Yun, et al. Simultaneous screening of 14 illegal food additives in wines using ultra performance liquid chromatography tandem mass spectrometry[J]. Chinese Journal of Analytical Chemistry, 2011, 39(11): 1732-1737.
[6] 刘进玺, 钟红舰, 董小海. 超高效液相色谱-质谱联用测定食品中叶酸含量[J]. 食品科学, 2012, 33(8): 233-235. LIU Jinxi, ZHONG Hongjian, DONG Xiaohai. Determination of folic acid in food by UPLC-MS/MS[J]. Food Science, 2012, 33(8): 233-235.
[7] 盛龙生. 代谢组学与中药研究[J]. 中国天然药物, 2008, 6(2): 98-102. SHENG Longsheng. Metabolomics and the research of Chinese traditional medicine[J]. Chinese Journal of Natural Medicines, 2008, 6(2): 98-102.
[8] Marcone MF. Characterization of the edible birds nest the "Caviar of the East"[J]. Food Res Int, 2005, 38(10): 1125-1134.
[9] Zhang Y, Imam MU, Ismail M, et al. Edible birds nest attenuates high fat diet-induced oxidative stress and inflammation via regulation of hepatic antioxidant and inflammatory genes[J]. BMC Complementary Altern Med, 2015, 15: 310. doi: 10.1186/s12906-015-0843-9.
[10] Yida Z, Imam MU, Ismail M, et al. Edible birds nest prevents high fat diet-induced insulin resistance in rats[J]. J Diabetes Res, 2015, 2015: 760535. doi: 10.1155/2015/760535.
[11] Yew MY, Koh RY, Chye SM, et al. Edible birds nest ameliorates oxidative stress-induced apoptosis in SH-SY5Y human neuroblastoma cells[J]. BMC Complementary Altern Med, 2014, 14: 391. doi: 10.1186/1472-6882-14-391.
[12] Matsukawa N, Matsumoto M, Bukawa W, et al. Improvement of bone strength and dermal thickness due to dietary edible birds nest extract in ovariectomized rats[J]. Biosci Biotechnol Biochem, 2011, 75(3): 590-592.
[13] Ma F, Liu D. Sketch of the edible birds nest and its important bioactivities[J]. Food Res Int, 2012, 48(2): 559-567.
[14] Hou Z, Imam MU, Ismail M, et al. Effects of edible birds nest on hippocampal and cortical neurodegeneration in ovariectomized rats[J]. Food Funct, 2015, 6(5): 1701-1711.
[15] Hou Z, Imam MU, Ismail M, et al. Lactoferrin and ovotransferrin contribute toward antioxidative effects of edible birds nest against hydrogen peroxide-induced oxidative stress in human SH-SY5Y cells[J]. Biosci Biotechnol Biochem, 2015, 79(10): 1570-1578.
[16] Haghani A, Mehrbod P, Safi N, et al. In vitro and in vivo mechanism of immunomodulatory and antiviral activity of edible birds nest(EBN)against influenza A virus(IAV)infection[J]. J Ethnopharmacol, 2016, 185: 327-340. doi: 10.1016/j.jep.2016.03.020.
[17] Guo CT, Takahashi T, Bukawa W, et al. Edible birds nest extract inhibits influenza virus infection[J]. Antiviral Res, 2006, 70(3): 140-146.
[18] Chua KH, Lee TH, Nagandran K, et al. Edible birds nest extract as a chondro-protective agent for human chondrocytes isolated from osteoarthritic knee: in vitro study[J]. BMC Complementary Altern Med, 2013, 13: 19. doi: 10.1186/1472-6882-13-19.
[19] Careena S, Sani D, Tan SN, et al. Effect of edible birds nest extract on lipopolysaccharide-induced impairment of learning and memory in Wistar rats[J]. Evid Based Complement Altern Med, 2018, 2018: 9318789. doi: 10.1155/2018/9318789.
[20] Abidin FZ, Hui CK, Luan NS, et al. Effects of edible birds nest(EBN)on cultured rabbit corneal keratocytes[J]. BMC Complementary Altern Med, 2011, 11: 94. doi: 10.1186/1472-6882-11-94.
[21] 董建辉, 田巧基, 段素芳, 等. 燕窝提取物的抗氧化及促进表皮细胞生长活性比较[J]. 食品与发酵工业, 2019, 45(17): 73-78. DONG Jianhui, TIAN Qiaoji, DUAN Sufang, et al. Comparative study on anti-oxidation and promotion activity of epidermal cell growth of edible birds nest water extract[J]. Food Ferment Ind, 2019, 45(17): 73-78.
[22] Li N, Ma Z, Li M, et al. Natural potential therapeutic agents of neurodegenerative diseases from the traditional herbal medicine Chinese Dragons Blood[J]. J Ethnopharmacol, 2014, 152(3): 508-521.
[23] 连建梅, 范群艳, 李红卫. 不同加工工艺对燕窝产品唾液酸含量的影响[J]. 食品工业科技, 2017, 38(1): 265-268, 277. LIAN Jianmei, FAN Qunyan, LI Hongwei. Influence of different processing technology on sialic acid content of edible birds nest products [J]. Science and Technology of Food Industry, 2017, 38(1): 265-268, 277.
[24] Cajka T, Fiehn O. Toward merging untargeted and targeted methods in mass spectrometry-based metabolomics and lipidomics[J]. Anal Chem, 2016, 88(1): 524-545.
[25] Trygg J, Holmes E, Lundstedt T. Chemometrics in metabonomics[J]. J Proteome Res, 2007, 6(2): 469-479.
[1] 陈炳荣,施勇旺,李嘉浩,翟吉良,刘梁,刘文勇,胡磊,赵宇. 基于支持向量机的脊柱离体组织电阻抗识别[J]. 山东大学学报 (医学版), 2023, 61(3): 57-62.
[2] 黄珊,娄能俊,韩晓琳,梁中昊,华梦羽,庄向华,陈诗鸿. 高糖环境下Lipin1对神经元代谢组学的影响[J]. 山东大学学报 (医学版), 2023, 61(2): 1-8.
[3] 苑宝文,王沛,黄蔚. 组蛋白去乙酰化酶SIRT1对胰腺癌代谢的调控作用[J]. 山东大学学报 (医学版), 2022, 60(3): 1-12.
[4] 章海容, 张小红, 王超群. 哺乳动物雷帕霉素靶蛋白通路调控ECA109细胞放疗敏感性的代谢组学[J]. 山东大学学报 (医学版), 2020, 58(1): 6-12.
[5] 于斐,刘少壮,仲明惟,黄鑫,焦杰,胡三元,于文滨. 基于GC-TOF-MS的结直肠癌代谢组学差异分析[J]. 山东大学学报(医学版), 2016, 54(7): 60-68.
[6] 刘盈君, 张涛, 王璐, 刘佳, 常学润, 张敬悬, 薛付忠. 基于随机森林的精神分裂症血清代谢组学研究[J]. 山东大学学报(医学版), 2015, 53(2): 92-96.
[7] 张新新1,李雨1,纪玉佳2,王鹏2,张永清2,薛付忠1. 主成分-线性判别分析在中药药性识别中的应用[J]. 山东大学学报(医学版), 2012, 50(1): 143-146.
[8] 于连龙1,王菊生2,王淑娥1,张凤凤3,赵长峰1. 中老年血脂异常患者6种微量元素营养状况的主成分分析[J]. 山东大学学报(医学版), 2012, 50(1): 140-142.
[9] 高青松,薛付忠. 核主成分logistic回归模型在非线性关联分析中的应用[J]. 山东大学学报(医学版), 2011, 49(5): 140-.
[10] 蒋海强1,李运伦2,解君2. 基于高效液相色谱-电喷雾-飞行时间质谱联用技术的高血压病血浆代谢组学分析[J]. 山东大学学报(医学版), 2011, 49(10): 150-.
[11] 彭倩倩,薛付忠
. permutationPCA方法在病例对照关联
分析中不同遗传模型下的表现
[J]. 山东大学学报(医学版), 2009, 47(03): 94-97.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!