基于GC-TOF-MS的结直肠癌代谢组学差异分析

doi:10.6040/j.issn.1671-7554.0.2016.013

摘要/Abstract

摘要： 目的探讨结直肠癌(CRC)患者癌组织、癌旁黏膜及正常黏膜的代谢差异。方法选取25例CRC患者,分别取癌组织、癌旁黏膜和正常黏膜各1份,采用气相色谱-飞行时间-质谱(GC-TOF-MS)分析平台对组织样本进行代谢组学检测,3种组织间相互比较并采用主成分分析和正交偏最小二乘判别分析对数据建模,分析差异代谢物并在KEGG数据库中映射,找出相关代谢通路。结果与正常黏膜相比,癌组织中有47种差异代谢物(P<0.05, q<0.05),其半乳糖代谢、磷酸戊糖途径、谷氨酸和谷氨酰胺代谢及谷氨酸、丙氨酸和天冬氨酸代谢明显改变(P<0.05);癌旁黏膜中有3种差异代谢物(P<0.05, q<0.05),其半乳糖代谢明显改变(P<0.05)。癌组织与癌旁黏膜相比,有34种差异代谢物(P<0.05, q<0.05),其半乳糖代谢、磷酸戊糖途径、谷氨酸和谷氨酰胺代谢及谷氨酸、丙氨酸和天冬氨酸代谢明显改变(P<0.05)。结论 CRC癌组织中糖代谢和氨基酸代谢发生改变。

关键词: 代谢组学, 氨基酸代谢, 结直肠肿瘤, 糖代谢

Abstract: Objective To explore the metabolic differences of cancer tissues, adjacent mucosa and normal mucosa in patients with colorectal cancer(CRC). Methods A total of 25 CRC patients were selected and the 3 kinds of tissues were taken from each patient. Gas chromatography time-of-flight mass spectrometry(GC-TOF-MS)platform was used for metabolomics analysis. The tissues were compared among each other, and principal components analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS-DA)were used to analyze the data. Differential metabolites were mapped in the KEGG database to search for the related metabolic pathways. Results Compared with the normal mucosa, 47 metabolites were found different(P<0.05, q<0.05)in cancer tissues, and galactose metabolism, pentose phosphate pathway, glutamate and glutamine metabolism, glutamate, alanine and aspartate metabolism changed significantly(P<0.05). In the adjacent mucosa, 3 metabolites were found different(P<0.05, q<0.05), and galactose metabolism changed significantly(P<0.05). A total of 34 metabolites were found different(P<0.05, q<0.05)between the cancer tissues and the adjacent mucosa, and pentose phosphate pathway, glutamate and glutamine metabolism, glutamate, alanine and aspartate metabolism changed significantly(P<0.05). Conclusion The glucose metabolism and amino acid metabolism are changed in CRC tissues.

Key words: Metabolomics, Glucose metabolism, Colorectal neoplasms, Amino acid metabolism

中图分类号:

R605

于斐,刘少壮,仲明惟,黄鑫,焦杰,胡三元,于文滨. 基于GC-TOF-MS的结直肠癌代谢组学差异分析[J]. 山东大学学报（医学版）, 2016, 54(7): 60-68.

YU Fei, LIU Shaozhuang, ZHONG Mingwei, HUANG Xin, JIAO Jie, HU Sanyuan, YU Wenbin. Metabolomics analysis of colorectal cancer based on GC-TOF-MS[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(7): 60-68.

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