IRX5 mRNA在结直肠癌诊断及预后中的价值

doi:10.6040/j.issn.1671-7554.0.2015.1146

摘要/Abstract

摘要： 目的探讨易洛魁家族同源盒基因(IRX5)mRNA在结直肠癌组织中的表达与临床病理及预后的关系。方法收集17例炎症性肠病、48例增生性息肉、54例腺瘤、142例结直肠癌及癌旁正常组织手术切除标本,提取组织标本的总RNA,用实时定量PCR(RT-qPCR)方法测定IRX5 mRNA的表达水平,结合临床资料,对IRX5 mRNA的差异表达与结直肠癌患者临床病理信息和预后的相关性进行分析。结果在结直肠癌组织中IRX5 mRNA表达量明显高于其他各组。IRX5 mRNA的表达量与肿瘤大小、淋巴结转移和远处转移密切相关(P<0.05)。此外,IRX5 mRNA高表达组的无病生存率(DFS)和总生存率(OS)明显低于低表达组(P<0.001),并且高表达IRX5 mRNA为结直肠癌预后的独立危险因素(P=0.020, HR=1.863; 95%CI=1.101~3.152)。结论 IRX5 mRNA在结直肠癌组织中表达升高,并且其表达量与肿瘤的恶性程度及预后相关,提示IRX5 mRNA可作为结直肠癌诊断及预后的标志物。

关键词: mRNA, 结直肠肿瘤, 预后, 实时定量PCR, 易洛魁族同源盒基因

Abstract: Objective To investigate the expression and clinical significance of iroquois homeobox(IRX5)mRNA in colorectal cancer(CRC). Methods Expressions of IRX5 mRNA were analyzed in 17 cases of inflammatory bowel diseases, 48 cases of hyperplastic polyp, 54 cases of colorectal adenoma, 142 CRC cases and 142 paired adjacent non-tumorous tissues by quantitative real-time polymerase chain reaction(RT-qPCR). The correlation between IRX5 mRNA expression and prognosis was analyzed. Results IRX5 mRNA expression was significantly elevated in the CRC group when compared with other groups(all P<0.001). In CRC, up-regulation of IRX5 mRNA expression was significantly correlated with tumor size, lymph node metastasis, and distant metastasis(all P<0.05). CRC patients with high IRX5 mRNA expression had poorer disease-free survival(DFS)and overall survival(OS)than those with low IRX5 mRNA expression(log-rank test, P<0.001). Further multivariable Cox regression analysis suggested that increased expression of IRX5 mRNA was an independent prognostic indicator for CRC(P=0.020, HR=1.863, 95%CI=1.101-3.152). Conclusion IRX5 mRNA expression is elevated in CRC, and associated with malignancy and prognosis of CRC, indicating that it may serve as a useful diagnostic and prognostic predictor.

Key words: Iroquois homeobox 5, Colorectal neoplasms, mRNA, RT-qPCR, Prognosis

中图分类号:

R574

刘童,张欣,王传新. IRX5 mRNA在结直肠癌诊断及预后中的价值[J]. 山东大学学报（医学版）, 2016, 54(7): 69-74.

LIU Tong, ZHANG Xin, WANG Chuanxin. Significance of IRX5 mRNA in the diagnosis and prognosis of colorectal cancer[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(7): 69-74.

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