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山东大学学报 (医学版) ›› 2022, Vol. 60 ›› Issue (10): 49-56.doi: 10.6040/j.issn.1671-7554.0.2022.0360

• 临床医学 • 上一篇    下一篇

利用时间质谱检出312例6月龄内健康婴儿鼻咽部微生态菌群的分布特征

关恒云1,刘岚铮1,姜慧钰1,白爱英1,刘辉1,赵小冬2,高尚3,李娜1,阮师漫1   

  1. 济南市疾病预防控制中心 1.细菌性疾病检验所;2.免疫所;3.传染病所, 山东 济南 250021
  • 发布日期:2022-09-30
  • 通讯作者: 阮师漫. E-mail:jncdcrsm@jn.shandong.cn刘岚铮. E-mail:jncdcllz@jn.shangdong.cn
  • 基金资助:
    传染病预防控制国家重点实验室自主研究开放课题(2019SKLID310,2020SKLID307);济南市卫生健康委员会科技计划项目(2020-4-105)

Distribution characteristics of nasopharyngeal microbiota of 312 healthy infants under 6 months of age detected by MALDI-TOF MS

GUAN Hengyun1, LIU Lanzheng1, JIANG Huiyu1, BAI Aiying1, LIU Hui1, ZHAO Xiaodong2, GAO Shang3, LI Na1, RUAN Shiman1   

  1. 1. Bacterial Disease Inspection Laboratory;
    2. Immunology Institute;
    3. Institute for Infectious Diseases Control, Jinan Center for Disease Control and Prevention, Jinan 250021, Shandong, China
  • Published:2022-09-30

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摘要： 目的 利用基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)技术研究济南地区6月龄内健康婴儿鼻咽部微生态菌群分布特征,为鼻咽部微生态研究提供样本库信息。 方法 采集2019年11月至2020年4月济南地区312例6月龄内健康婴儿鼻咽拭子,增菌后,分离培养获得纯菌株;利用MALDI-TOF MS技术鉴定分离株到种置信水平;312例婴儿分为月龄≤2组、3月龄组、4月龄组和5月龄组,组间比较采用SPSS 22.0统计学软件,卡方或Fisher确切概率法统计分析。 结果 312例健康婴儿鼻咽拭子获得纯菌株741株,分属于25个属74种细菌。优势菌属为葡萄球菌属(31.6%)、棒杆菌属(30.8%)、链球菌属(18.9%)、莫拉菌属(8.6%)和狡诈球菌属(2.0%)。同时发现一些不常见菌种,例如缺陷乏氧菌、放射根瘤菌等。金黄色葡萄球菌检出率男婴(40.5%)高于女婴(26.4%),接近棒杆菌女婴(42.4%)高于男婴(27.4%)。4个年龄组中,假白喉棒杆菌检出率依次升高(10.8%、17.7%、18.9%、46.7%);肺炎链球菌检出率依次升高(2.2%、2.3%、4.1%、20.0%)。 结论 济南地区健康6月龄内婴儿鼻咽部微生态菌群以优势菌为主呈现多样化,MALDI-TOF MS是鉴定常见和不常见微生物菌种的快速、高通量技术手段。本研究可为济南地区儿童鼻咽部微生态研究提供有价值的样本库资料。

关键词: 鼻咽部, 基质辅助激光解吸电离飞行时间质谱, 微生态, 婴儿

Abstract: Objective To explore the distribution characteristics of nasopharyngeal microflora in healthy infants under 6 months of age using matrix-assisted laser desorption ionization time of flight mass spectrometry(MALDI-TOF MS)so as to provide sample bank information for nasopharyngeal microecology research. Methods Nasopharyngeal swabs were collected from 312 healthy infants during Nov. 2019 and April 2020 in Jinan. The pure strains were obtained after culturing and isolating. The bacterial strains were identified by MALDI-TOF MS to the species-level. The infants were divided into four age groups(≤ 2 months, 3 months group, 4 months group and 5 months group). SPSS 22.0 software was used for chi-square test or Fisher exact test between groups. Results A total of 741 bacterial strains were isolated, including 74 species of 25 genera of bacteria. The dominant genera were Staphylococcus(31.6%), Corynebacterium(30.8%), Streptococcus(18.9%), Moraxella(8.6%)and Dolosicoccus(2.0%). Some uncommon bacteria were also found, such as Abiotrophia defectiva, Rhizobium radiobacter, et al. The detection rate of Staphylococcus aureus was higher in male infants(40.5%)than in female infants(26.4%), while the detection rate of Corynebacterium propinquum was higher in female infants(42.4%)than in male infants(27.4%). In the four age groups, the detection rate of Corynebacterium pseudodiphtheriticum was increased successively(10.8%, 17.7%, 18.9% and 46.7%), as the same for Streptococcus pneumoniae(2.2%, 2.3%, 4.1% and 20.0%). Conclusion The diversity is mainly characterized by dominant bacteria in nasopharyngeal microflora for healthy infants under 6 months of age in Jinan, China. MALDI-TOF MS is a rapid and high-throughput technique for identifying common and uncommon bacterial species. The results can provide valuable reference for the study of nasopharyngeal microecology of children in Jinan.

Key words: Nasopharynx, Matrix assisted laser desorption ionization time of flight mass spectrometry, Micro-ecology, Infant

中图分类号: 

  • R183.3
[1] Group NHW, Peterson J, Garges S, et al. The NIH human microbiome project[J]. Genome Res, 2009, 19(12): 2317-2323.
[2] Integrative HMP(iHMP)Research Network Consortium. The integrative human microbiome project[J]. Nature, 2019, 569(7758): 641-648.
[3] 郑跃杰. 肺炎链球菌疾病与微生态平衡[J]. 中华实用儿科临床杂志, 2020, 35(7): 543-546. ZHENG Yuejie. Streptococcus pneumoniae diseases and microecological balance[J]. Chinese Journal of Applied Clinical Pediatrics, 2020, 35(7): 543-546.
[4] Croxatto A, Prod'hom G, Greub G. Applications of MALDI-TOF mass spectrometry in clinical diagnostic microbiology[J]. FEMS Microbiol Rev, 2012, 36(2): 380-407.
[5] Patel R. MALDI-TOF MS for the diagnosis of infectious diseases[J]. Clin Chem, 2015, 61(1): 100-111.
[6] Torres-Sangiao E, Leal Rodriguez C, García-Riestra C. Application and perspectives of MALDI-TOF mass spectrometry in clinical microbiology laboratories[J]. Microorganisms, 2021, 9(7): 1539.
[7] 罗春绸, 蔡惠贞, 张月葵, 等. 健康学龄前儿童入幼儿园前后鼻咽部病原学变化的队列研究[J]. 中国循证儿科杂志, 2019, 14(3): 201-204. LUO Chunchou, CAI Huizhen, ZHANG Yuekui, et al. Cohort study on pathogenic changes of nasopharyngeal in preschool children before and after going to kindergarten[J]. Chinese Journal of Evidence-Based Pediatrics, 2019, 14(3): 201-204.
[8] 华春珍, 赵丽娅, 宋培红, 等. 657名学龄前儿童肺炎链球菌带菌率调查[J]. 中华流行病学杂志, 2004, 25(10): 915-916.
[9] 罗小铭, 梁建平, 高赛珍, 等. 中山市186名儿童鼻咽部带菌状况调查[J]. 中国微生态学杂志, 2006, 18(3): 204-205. LUO Xiaoming, LIANG Jianping, GAO Saizhen, et al. Surveys on bacteria nasopharyngeal carriage prevalence in 186 children[J]. Chinese Journal of Microecology, 2006, 18(3): 204-205.
[10] Teo SM, Mok D, Pham K, et al. The infant nasopharyngeal microbiome impacts severity of lower respiratory infection and risk of asthma development[J]. Cell Host Microbe, 2015, 17(5): 704-715.
[11] Biesbroek G, Wang XH, Keijser BJF, et al. Seven-valent pneumococcal conjugate vaccine and nasopharyngeal microbiota in healthy children[J]. Emerg Infect Dis, 2014, 20(2): 201-210.
[12] Satzke C, Turner P, Virolainen-Julkunen A, et al. Standard method for detecting upper respiratory carriage of Streptococcus pneumoniae: updated recommendations from the world health organization pneumococcal carriage working group[J]. Vaccine, 2013, 32(1): 165-179.
[13] Aguirre M, Morrison D, Cookson BD, et al. Phenotypic and phylogenetic characterization of some Gemella-like organisms from human infections: description of Dolosigranulum pigrum gen. nov., sp. nov[J]. J Appl Bacteriol, 1993, 75(6): 608-612.
[14] Bosch AATM, Levin E, van Houten MA, et al. Development of upper respiratory tract microbiota in infancy is affected by mode of delivery[J]. EBio Medicine, 2016, 9: 336-345. doi: 10.1016/j.ebiom.2016.05.031.
[15] Bosch AATM, de Steenhuijsen Piters WAA, van Houten MA, et al. Maturation of the infant respiratory microbiota, environmental drivers, and health consequences. A prospective cohort study[J]. Am J Respir Crit Care Med, 2017, 196(12): 1582-1590.
[16] 王立波, 张明智, 冯海燕, 等. 社区获得性肺炎患儿鼻咽部病原年龄分布特点[J]. 中国抗感染化疗杂志, 2005, 5(4): 218-221. WANG Libo, ZHANG Mingzhi, FENG Haiyan, et al. Distribution of nasopharyngeal pathogen by age group in children with community-acquired pneumonia[J]. Chinese Journal of Infection and Chemotherapy, 2005, 5(4): 218-221.
[17] Hardy BL, Dickey SW, Plaut RD, et al. Corynebacterium pseudodiphtheriticum exploits staphylococcus aureus virulence components in a novel polymicrobial defense strategy[J]. mBio, 2019, 10(1): e02491-18. doi:10.1128/mBio.02491-18.
[18] Humphreys H, Fitzpatick F, Harvey BJ. Gender differences in rates of carriage and bloodstream infection caused by methicillin-resistant Staphylococcus aureus: are they real, do they matter and why?[J]. Clin Infect Dis, 2015, 61(11): 1708-1714.
[19] McClelland EE, Smith JM. Gender specific differences in the immune response to infection[J]. Arch Immunol Ther Exp, 2011, 59(3): 203-213.
[20] Tilouche L, Ben Dhia R, Boughattas S, et al. Staphylococcus aureus ventilator-associated pneumonia: a study of bacterio-epidemiological profile and virulence factors[J]. Curr Microbiol, 2021, 78(7): 2556-2562.
[21] Castleman MJ, Pokhrel S, Triplett KD, et al. Innate sex bias of Staphylococcus aureus skin infection is driven by α-hemolysin[J]. J Immunol, 2018, 200(2): 657-668.
[22] Oviaño M, Rodríguez-Sánchez B. MALDI-TOF mass spectrometry in the 21st century clinical microbiology laboratory[J]. FEMS Microbiol Rev, 2012, 36(2): 380-407.
[23] Jangda U, Upadhyay A, Bagheri F, et al. Corynebacterium propinquum: a rare cause of prosthetic valve endocarditis[J]. Case Rep Med, 2016, 2016: 1391789. doi:10.1155/2016/1391789.
[24] Malkoçoglu G, Gencer H, Kaya A, et al. Corynebacterium propinquum bronchopneumonia in a child with Ataxia telangiectasia[J]. Turkish J Pediatr, 2016, 58(5): 558-561.
[25] Kawamura Y, Hou XG, Sultana F, et al. Transfer of Streptococcus adjacens and Streptococcus defectivus to Abiotrophia gen. nov. as Abiotrophia adiacens comb. nov. and Abiotrophia defectiva comb. nov., respectively[J]. Int J Syst Bacteriol, 1995, 45(4): 798-803.
[26] Bozkurt I, Coksevim M, Cerik IB, et al. Infective endocarditis with atypical clinical feature and relapse by Abiotrophia defective[J]. J Saudi Heart Assoc, 2017, 29(2): 136-138.
[27] 许凌, 林雷, 陈龙, 等. 缺陷乏养菌致感染性心内膜炎1例[J]. 山西医科大学学报, 2018, 49(1): 87-89.
[28] 邵小华, 彭静. 缺陷乏氧菌引起的亚急性感染性心内膜炎1例[J]. 安徽医学, 2019, 40(3): 347-348. doi: 10.3969/j.issn.1000-0399.
[29] 周庭银, 章强强. 临床微生物学诊断与图解[M]. 4版. 上海: 上海科学技术出版社, 2017.
[30] Hashiba T, Ono Y, Mise N. Rhizobium radiobacter peritonitis in a peritoneal dialysis patient: case presentation and review of the literature[J]. Ther Apher Dial, 2021, 25(3): 358-360.
[31] Chen CY, Hansen KS, Hansen LK. Rhizobium radiobacter as an opportunistic pathogen in central venous catheter-associated bloodstream infection: case report and review[J]. J Hosp Infect, 2008, 68(3): 203-207.
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