山东大学学报 (医学版) ›› 2019, Vol. 57 ›› Issue (3): 13-18.doi: 10.6040/j.issn.1671-7554.0.2018.661
• • 上一篇
王伟,刘拥征,李岭
WANG Wei, LIU Yongzheng, LI Ling
摘要: 目的 探讨酸浆苦素B(PB)对非小细胞肺癌(NSCLC)A549细胞增殖、迁移和凋亡的影响,初步探讨其作用机制。 方法 细胞分为不同浓度(2.5、5、10、20、40 μmol/L)PB组、对照组、空白组,实时无标记细胞分析技术(RTCA)、CCK-8法检测PB对A549细胞增殖抑制率变化,Transwell检测细胞迁移,流式细胞仪检测PB对A549细胞的凋亡及周期的影响,Western blotting检测PB对相关蛋白表达的影响。 结果 2.5、5、10、20、40 μmol/L PB可抑制A549细胞增殖,且呈剂量依赖性;Transwell结果显示,10、20 μmol/L PB组较对照组的细胞迁移能力下降;10、20 μmol/L PB处理后,A549细胞的凋亡率升高,呈剂量依赖性。与对照组相比,10、20 μmol/L PB组G2/M期细胞比例增高、G0/G1期比例减少。Western blotting检测结果显示,与对照组相比,10、20 μmol/L PB组使Akt蛋白表达水平下降,而Caspase-9和PTEN蛋白表达水平上升。 结论 PB可抑制A549细胞的增殖,诱导细胞凋亡且细胞周期阻滞,这可能与调控细胞周期相关蛋白表达有关。
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