干扰PRPS2基因表达对宫颈癌细胞生物学特性的影响

doi:10.6040/j.issn.1671-7554.0.2017.952

摘要/Abstract

摘要： 目的探讨磷酸核糖焦磷酸合成酶2(PRPS2)对宫颈癌细胞HeLa生长增殖、迁移侵袭以及细胞周期和凋亡的影响。方法采用Western blotting法检测宫颈癌细胞HeLa和正常表皮细胞中PRPS2蛋白的基础表达。应用Lipofectamine 2000将PRPS2特异性小干扰RNA(siPRPS2)转染至人宫颈腺癌细胞(HeLa)中,并设置相应对照组,采用Western blotting法检测PRPS2的干扰效率;采用Cell Counting Kit8细胞增殖试剂盒(CCK-8)、细胞克隆实验和Transwell侵袭实验检测细胞生长增殖、迁移和侵袭能力;采用流式细胞术检测PRPS2对细胞周期和凋亡的影响。结果 PRPS2在HeLa细胞中呈高表达,siPRPS2可有效抑制HeLa细胞PRPS2蛋白的表达(抑制率为70%);CCK-8和克隆实验显示干扰PRPS2表达后,HeLa细胞的活性和增殖能力明显下降(P<0.001, P<0.01);Transwell实验表明,敲低PRPS2后,HeLa细胞迁移和侵袭至下室的细胞数明显减少(P<0.05, P<0.001);敲低PRPS2后HeLa细胞周期发生G2阻滞(P<0.001),并且细胞凋亡比例升高(P=0.012)。结论 PRPS2可促进宫颈癌细胞的生长增殖、侵袭转移和推动细胞周期进程。

关键词: 宫颈癌, 磷酸核糖焦磷酸合成酶2, 增殖, 迁移, 侵袭

Abstract: Objective To evaluate the role of phosphoribosyl-pyrophosphate synthetase 2(PRPS2)on the proliferation, metastasis, cell cycle and cell apoptosis of cervical cancer cells. Methods The basal expressions of PRPS2 in cervical cancer cells(HeLa cells)and normal epithelial cells were detected with Western blotting. HeLa cells were transfected with PRPS2-specific siRNA(siPRPS2)via Lipofectamine 2000, and then divided into the knockdown group and control group. The knockdown efficiency of PRPS2 was determined with Western blotting. The cell proliferation, migration and invasion were detected with CCK-8, colony formation and Transwell assays. The effect of PRPS2 on cell cycle and apoptosis were examined with flow cytometry. Results PRPS2 was highly expressed in HeLa cells, and siPRPS2 effectively inhibited the expression of PRPS2(inhibition ratio 70%). CCK-8 and colony formation assays indicated that PRPS2 knockdown significantly inhibited the viability(P<0.001)and proliferation(P<0.01)of HeLa cells. Transwell assays showed that PRPS2 knockdown obviously reduced the number of migrated(P<0.05)and invaded(P<0.001)cells through the membrane. PRPS2 knockdown contributed to G2/M arrest(P<0.001)and apoptosis of cervical cancer cells(P=0.012). Conclusion PRPS2 can promote the proliferation, metastasis and cell cycle of cervical cancer cells.

Key words: Cervical cancer, Phosphoribosyl-pyrophosphate synthetase 2, Proliferation, Migration, Invasion

中图分类号:

R737

陈颖,周小青,齐眉,张魏芳,刘娟,赵蔚明. 干扰PRPS2基因表达对宫颈癌细胞生物学特性的影响[J]. 山东大学学报 (医学版), 2018, 56(2): 9-13.

CHEN Ying, ZHOU Xiaoqing, QI Mei, ZHANG Weifang, LIU Juan, ZHAO Weiming. Effects of PRPS2 knockdown on the biological property of cervical cancer cells[J]. Journal of Shandong University (Health Sciences), 2018, 56(2): 9-13.

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