伯氏疏螺旋体诱导大鼠类风湿关节炎模型的建立

doi:10.6040/j.issn.1671-7554.0.2015.019

摘要/Abstract

摘要： 目的应用伯氏疏螺旋体全菌蛋白首次皮内注射,再次腹腔注射加强免疫诱导Lewis大鼠类风湿关节炎(RA)模型并通过检测血清细胞因子探讨该模型发病机制。方法将雄性Lewis大鼠54只随机分为螺旋体低剂量组、螺旋体中剂量组、螺旋体高剂量组、完全弗氏佐剂组、不完全弗氏佐剂组及PBS组,每组9只。采用关节炎指数和组织病理学方法观察大鼠的发病程度和病理特点。采用双抗体夹心ELISA法检测血清RF、TNF-α、IL-1β、IL-6和IL-17含量。结果螺旋体高剂量组大鼠关节炎发病率为66.7%。病变关节病理显示关节滑膜组织增生,炎性细胞浸润,软骨及骨质侵蚀,呈典型的关节炎改变。螺旋体高剂量组与完全弗氏佐剂组相比关节炎指数、组织病理学评分及血清RF、TNF-α、IL-1β、IL-6和IL-17水平的差异无统计学意义(P>0.05)。螺旋体高剂量组与PBS组相比关节炎指数、组织病理学评分及血清RF、TNF-α、IL-1β、IL-6和IL-17水平明显升高(P<0.05)。结论伯氏疏螺旋体全菌蛋白成功诱导大鼠关节炎,与人类RA的发病及病理特点极为相似,是研究RA较理想的模型之一。

关键词: 关节炎, 模型, 自身免疫, 伯氏疏螺旋体, 全菌蛋白, 动物, 类风湿

Abstract: Objective To establish rat models of rheumatoid arthritis by primary intradermal and secondary intraperitoneal injections of whole spirochaeta protein of Borrelia burgdorferi (B.b), and to explore the pathogenesis of the disease. Methods A total of 54 Lewis rats were randomly divided into low-dose B.b group, medium-dose B.b group, high-dose B.b group, complete Freunds adjuvant group, incomplete Freunds adjuvant group and PBS group, with 9 rats in each group. The degree of pathogenicity and pathological characteristics were assayed with arthritis index and histiopathological techniques. Serum cytokines were measured with enzyme linked immunosorbent assay (ELISA). Results The incidence of arthritis in high-dose B.b group was 66.7%. Histopathological tests showed typical arthritis pathology, including synovial proliferation, articular cartilage and bone erosion. The arthritis index, histological score and cytokine expressions of the high-dose B.b group had no statistical difference compared with those of the complete Freunds adjuvant group (P>0.05), but they were significantly higher than those of the PBS group (P<0.05). Conclusion Arthritis induced by whole spirochaeta protein of B.b mirrors human rheumatoid arthritis and is one of the ideal animal models for studying rheumatoid arthritis.

Key words: Animal, Borrelia burgdorferi, Model, Whole spirochaeta protein, Rheumatoid, Arthritis, Autoimmunity

中图分类号:

R593.22

王威严, 杨清锐, 师健民, 赵璐莲, 张源潮. 伯氏疏螺旋体诱导大鼠类风湿关节炎模型的建立[J]. 山东大学学报（医学版）, 2015, 53(9): 47-52.

WANG Weiyan, YANG Qingrui, SHI Jianmin, ZHAO Lulian, ZHANG Yuanchao. Establishment of rat models of rheumatoid arthritis induced by Borrelia burgdorferi[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(9): 47-52.

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