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Table of Content

      
    16 April 2008
    Volume 46 Issue 4
    Articles
    Articles
    Effect of ACE2 over-expression on cardiac function in diabetic cardiomyopathy rats
    YU Qing-tao,GAO Yan-yan,ZHANG Lei,DAI Hong-yan,YANG Ya-pei,DONG Qiu-li,LIU Bin,ZHANG Yong-huan,DONG Bo
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(4):  331-334. 
    Abstract ( 1541 )   PDF (337KB) ( 705 )   Save
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    To explore the effect of adenoviral gene transfer of angiotensin converting enzyme-2（ACE2） on diabetic cardiomyopathy rats. MethodsDiabetes was induced by a single intraperitoneal injection of STZ in male Wistar rats. After 12 weeks, the rats were randomly divided into three groups: the control group, the EGFP group and the ACE2 group. Sterile saline, recombinant adenoviral carrying enhanced green fluorescent protein gene (AdV5.eGFP) and recombinant adenoviral carrying mouse ACE2 gene (AdV5.ACE2) were respectively injected to myocardia in different groups. 10 days after gene transfection, myocardial ACE2 mRNA expression was determined. 30 days after gene transfection , left ventricular function, myocardial collagen content and myocardial AngII level were also determined. ResultsCompared with the control and EGFP groups, ACE2mRNA (P<0.05) and cardiac function (P<0.05) were significantly increased in the ACE2 group while myocardial collagen content (P<0.01) and AngII level were significantly decreased in the ACE2 group(P<0.01). ConclusionsOverexpression of ACE2 may have therapeutic potentials in improving cardiac function.
    Correlation between airway inflammation and asthma marine remodeling and corticosteroid resistence in rats with respiratory syncial virus infection
    YANG Yan-na,CHEN Fang-fang,LI Wei,WANG Yan,WANG Xing-guang,JIANG Shu-juan
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(4):  335-339. 
    Abstract ( 1467 )   PDF (421KB) ( 550 )   Save
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    To explore the relationship of airway inflammation and murine model to corticosteroid resistance after respiratory syncytial virus (RSV) infection. Methods 50 BALB/c mice were divided into 5 groups: the control group, the OVA group（group A）, the OVA+Dex group（group B）, the OVA/RSV group(group C), and the OVA/RSV+ Dex group（group D）, 10 mice in each group. Then the concentrations of the interleukin 4（IL-4）, interleukin 5（IL-5）, interferon-γ（IFN-γ）and transforming growth factor-β1(TGF-β1) were determined by ELISA. Pathological slides were stained with hematoxylineosin and Van. Gieson, and degree of the peribronchial inflammation and airway collage deposition were observed. ResultsCompared with the control group, concentrations of IL-4, IL-5 and TGF-β1 were significantly increased in groups A, C and D and airway inflammation and collagen deposition were also aggravated（P＜0.05）. Also concentrations of IL-4, IL-5 and TGF-β1 and airway inflammation and remodeling were gradually increased in the given order of groups A, C and D（P＜0.05）. Conclusion RSV infection results in a significant Th2 inflammation and airway remodeling in rats with asthma, and the rats are not sensitive to corticosteroid treatment.
    Potential biomarkers in RRMS
    BAI Shu-mei,LIU Shi-lian,YANG Yin-rong,GUO Xu-xiao,QIN Yan-jiang,DENG Xiao-mei
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(4):  340-343. 
    Abstract ( 1326 )   PDF (377KB) ( 454 )   Save
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    Comparative proteomics and biological signaling network analysis were carried out in the cerebrospinal fluid (CSF) of patients with relapsingremitting MS (RRMS) to investigate the relationships among the potential biomarkers and between the biomarkers and RRMS. MethodsCSF of patients with RRMS and the controls was collected and determined by the 2-dimensional electrophoresis(2-DE). The differential expression protein spots were selected with the Image Master 2D-gel software and identified with the matrix assisted laser desorption ionization time of flight mass spectrometry(MALDI-TOF-MS). ELISA was performed to verified one of the protein spots, Cystatin C. Finally the correlations of these proteins were analyzed by MetaCore integrated software. ResultsThere were eight protein spots expressed differentially on the 2-DE maps, in which four upregulated and four downregulated proteins were identified in RRMS. Cystatin C was downregulated and further confirmed by the ELISA assay(4.36±1.22mg／L, 6.00±1.68mg／L, P＜0.01). A map of signaling network about these proteins was built by MetaCore integrated software. ConclusionThe differential expressions and biological signaling network researches of the proteins assist in exploring the pathogenesis, differential diagnosis and drug targets of RRMS on molecular level.
    Separation and identification of myrrh sesquiterpenoids and its anti-proliferation effect on tumor cell
    JI Kai,KONG Feng,SHEN Tao,WANG Xiao-ling,XU Ai-hu,YUAN Hui-qing,ZHANG Xiu-tian
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(4):  344-348. 
    Abstract ( 1585 )   PDF (348KB) ( 553 )   Save
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    To determine the structures of two sesquiterpene compounds isolated from Commiphora opobalsamum, and to investigate its anti-proliferation effect on tumor cell growth. Methods Two sesquiterpenoids were isolated from the resinous exudates of Commiphora opobalsamum. Their structures were established on the basis of physical and chemical properties, and 1H-NMR and 13 Results Structures of two sesquiterpenoids were determined as (1(10)E,2R,4R)-2-methoxy-8, 12-epoxygermacra-1(10), 7, 11-trien-6-one and 2-methoxy-5-acetoxyfuranogermacr-1(10)-en-6-one, respectively. The results of MTT assay showed that both two compounds had an inhibitory effect on proliferation of the hormone-independent prostate cancer cell lines in a concentration-dependent manner. While no significant anti-proliferation activity was observed in colorectal cells exposed to these chemicals at low concentrations. Furthermore, less cytotoxicity of these compounds was shown by using immortalizied liver normal cell line at concentrations tested. Conclusions Two germacrane sesquiterpenoids were identified the structures, and they can significantly inhibit the proliferation of hormone-independent prostate cancer cell growth.
    Reversal of multi-drug resistance in human breast cancer MCF-7/ADR cells by short hairpin RNA expression plasmids
    WEI Jun-min,HOU Ming,LI Li-zheng,KONG Feng,BIAN Ji-feng
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(4):  349-352. 
    Abstract ( 1337 )   PDF (305KB) ( 684 )   Save
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    To construct the shRNA plasmids which targeting the MDR1 Gene and to investigate their reversal effect on human breast cancer cell line MCF-7/ADR. MethodsThe oligonucleotides of the MDR-1 gene were designed based on that of the Gene Bank, and the MDR-1 shRNA expression plasmid pRNATU6.1/Neo-mdr1 was constructed. Human breast cancer cell line MCF-7/ADR was tranfected with the MDR-1-shRNA expression plasmids by lipofectamine 2000, and positive clone was selected by G418. MDR-1mRNA was assayed by RT-PCR and protein expression was determined by Western blotting. The P-gp function was determined by rhodamine 123 retention and the efficiency of MCF7/ADR to ADM was determined by MTT method. ResultsThe MDR-1 shRNA expression plasmids were successfully constructed .The MDR1mRNA and protein expressions were significantly decreased 48 hours after transfection, 1 month and 2 months after selection by G418， and that the sensitivity to P-gp-transportable drugs was restored. The transporting function of P-gp was increased and the efficiency of MCF-7/ADR to ADM significantly reversed. Conclusion The shRNA expression plasmid pRNATU6.1/Neo-mdr1 can inhibit the MDR-1 gene stably and permanently. The sensitivity of MCF-7/ADR to adriamycin is reversed.
    Effect of the Yishenhuoxue capsule on MCP-1 mRNA expression and NF-κB activation in atherosclerosis induced by hyperhomocysteinemia in rabbits
    LIU Fen-ye,ZHANG Ji-dong,HU Lian-hai,LAI Li-ping,YANG Fa-lin
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(4):  353-356. 
    Abstract ( 1180 )   PDF (327KB) ( 361 )   Save
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    To explore the effects of the Yishenhuoxue capsule (YC) on aortic monocyte chemoattractant protein-1 (MCP-1) mRNA expression and nuclear factor-kappaB (NF-κB) activation in atherogenesis of hyperhomocysteinemic rabbits. Methods Forty male New Zealand white rabbits were divided into five groups: the control, the model, the low dose YC, the high dose YC and the western medicine (folic acid, VitB6 and VitB12) group. Apart from the control group, rabbits in other groups underwent balloon catheter injuries and a high methionine diet to induce hyperhomocysteinemia and atherosclerosis by treatment with different drugs. The plasma Hcy concentration, the aortic morphological changes, the MCP-1 mRNA expression and the expression of inhibitor of nuclear factor-kappaB (IκBα) protein in the abdominal aorta were respectively determined by high performance liquid chromatography, HE staining, real-time quantitative PCR and western blot. Results Both YC and western medicine could decrease the plasma Hcy concentration（P＜0.01), attenuate the intimal thickening, down-regulate the MCP-1 mRNA expression（P＜0.01) and increase the IκBα protein expression（P＜0.01). The efficacy of YC at a high dose was superior to that of YC at a low dose andwestern medicine（P＜0.05). Conclusion YC decreases the plasma Hcy level and subsequently reverses the aortic lesion induced by Hcy. The mechanisms may be related to its suppressive effects on MCP-1.
    Effect of SS-GAG on ET1 and NO secretion of vascular endothelial cells damaged by highly concentrated glucose
    WANG Rui-xin,WANG Chun-bo,LUO Shi-bin,LI Yu-qiang
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(4):  357-361. 
    Abstract ( 1364 )   PDF (448KB) ( 513 )   Save
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    To study the effects of scallop skirt glycosaminoglycan (SS-GAG) on ET1 and NO secretion of ECV304 damaged by highly concentrated glucose. Methods The vascular endothelial cell ECV304 was cultured in vitro, and the damage mode1 of endothelia1 cells was established by highly concentrated glucose．Different concentrations of SS-GAG was mixed with cell culture fluid containing highly concentrated glucose (55.5mmol/L), and the cell morphology was observed after cultured for 12h, 24h, 36h and 48h, respectively. Nitric oxide (NO) inside cells were determined by the assay of nitric acid reductase. Radioimmunoassay was used to determine ET-1. ResultsCell morphology was changed and proliferation was depressed under the highly concentrated glucose, and a dose-dependent reaction occurred. SSGAG weakened the damage effects of highly concentrated glucose, and made these cells keep their normal morphology. The concentration of ET-1 in the cell culture supernatant was increased compared with the normal control group. Differences had statistics meaning compared with the normal control group (P＜0.01). And the NO inside cells initially increased, and then reduced. Differences had statistics meaning compared with the normal control group (P＜0.01). There were obvious differences between the SS-GAG group and the negative control group. With modulation of SS-GAG, ET-1 and NO inclined to the normal level,difference occurred between the SSGAG and the highly concentrated glucose groups. Difference had statistics meaning compared with the highly concentrated glucose group (P＜0.01) but not with the normal control group (P＞0.05). ConclusionsHighly concentrate glucose has obvious damaged effects on vascular endothelial cells, which may induce the cell morphology change, dysfunction, ET1 increase and NO secretion. SS-GAG will weaken this damage and protect the cells by modulating the balance of ET1 and NO.
    Effect of Losartan and A779 on myocardial interstitial fibrosis in diabetic cardiomyopathy
    HU Sheng,ZHANG Xu-hong,DONG Bo,ZHU Mei,ZHANG Yue-hui,YU Qing-tao
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(4):  362-365. 
    Abstract ( 1364 )   PDF (325KB) ( 518 )   Save
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    To investigate the effect of Losartan and A779 on myocardial interstitial fibrosis in diabetic cardiomyopathy (DCM). MethodsThirtynine male Wistar rats were intraperitoneally injected with streptozocin (STZ) once to induce hyperglycemia. After 12 weeks of STZ injection, rats whose blood glucose was higher than 16.7mmol/L and who had polydipsia, hyperphagia and polyuria were randomly divided into 3 groups: the DCM group, the losartan group and the A779+Losartan group. The DCM group was subjected to perfusion of normal saline into the stomach once daily, the losartan group perfusion of losartan once daily, and the A779+Losartan group perfusion of losartan and injection of A779 through the tail vein once daily. At the end of the experiment, LVIDs, LVIDd, E/A, LVEF and FS were determined by ultrasound. Then the collagen level was determined by Masson staining, and the mRNA expression of angiotensinconvertion enzyme 2 (ACE2) was determined by real-time RT-PCR. ResultsBy the end of the experiment, the LVIDs, LVIDd and the collagen level of the losartan group and the A779+Losartan group were lower than those of the DCM group (P＜0.01), also the E/A, FS, LVEF levels and the expression of ACE2 mRNA of the losartan group and the A779+Losartan group were higher than those of the DCM group(P＜0.01). The LVIDs, LVIDd and collagen content of the A779+Losartan group were higher than those of the losartan group(P＜0.05), and E/A, FS and LVEF of the A779+Losartan group were lower than those of the losartan group(P＜0.05). However the expression of ACE2 mRNA had no difference between the losartan group and the A779+Losartan group. ConclusionsLosartan amelioorates the myocardial interstitial fibrosis via Ang-(1.7).
    Effects of Cilostazol on the learning and recalling abilities and the caspase-3 expression after global cerebral ischemiareperfusion in rats
    LI Yun,YUAN Xiao-dong,OUYANG He-zhong,ZHANG Yi-chao,ZHU Mei-rong
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(4):  366-369. 
    Abstract ( 1277 )   PDF (343KB) ( 382 )   Save
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    To explore the effects of Cilostazol on the learning and recalling ability and caspase-3 expression after global cerebral ischemia-reperfusion in rats. MethodsTransient global cerebral ischemia was induced by the fourvessel occlusion in SpragueDawley(SD) rats.SD rats were randomly divided into sham-operated group（n=8×1）, model group(n=8×7) and Cilostazol-treating group(n=8×7). Rats in treatment group received two oral administration of cilostazol at 6h and 2h before the global cerebral ischemia，then they were given cilostazol every 24h.　Rats in other groups were given the same volume of 30% dimethyl sulfoxide. The rats in model control group and treatment group were decapitated at 6、12、24、48、72h、5d and 7d after 10min cerebral ischemia. The expression of caspase3 in CA1 region of hippocampus were detected by the method of immunohistochemistry. The another 24 rats were randomly divided into 3 groups:shamoperated group(n=8), ischemia reperfusion group (n=8)　and treatment group(n=8).　They were used to study the learning and recalling ability of rats at 7?d after global ischemia-reperfusion by Morris water maze.Results Caspase-3 was scarecely expressed in shamoperated group；while in model group, it increased at 6 hour, peaked at 72 hour, and deceased nearby to the base lever at 7 day；for caspase-3 mean positive cells in CA1 region, they were obviously lower in 48、72?h、5?d　and 7?d subgroups of treatment group than those in model group（P＜0.05）. Within 5 days, the Escape Latency(EL) in ischemia reperfusion group and treatment group was markedly longer than that of in shamoperated group(P＜0.01, P＜0.05 respectively)；within 4 days, the Escape Latency(EL) in treatment group was markedly shorter than that of in ischemia reperfusion group(P＜0.01);whereas on the fifth day,there was no significant difference in these two groups.Conclusion Cilostazol can effectively inhibited the increase of caspase3 levels which may be one mechanism of its increasing the learning and recalling ability of rats.
    Protective effect of gypenosides-containing serum against oxidation neurotoxicity induced by glutamate
    LIU Lu-hua,LU Su-mei,SUN Tao,ZHANG Dao-lai,FENG Yu-xin,XIN Hua
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(4):  370-373. 
    Abstract ( 1535 )   PDF (406KB) ( 918 )   Save
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    To investigate the neuroprotective effects of gypenosides (GP) containing serum on glutamate (Glu) induced oxidation toxicity to cortical neurons. Methods Sera containing GP was collected by ways of seropharmacology, and the primary cortical neurons isolated from Wistar rats E1415d embryos were studied. The viability of cells was detected by the MTT method, the cellular death pathway was analyzed by Ho33342 and PI doublelabeled method, the contents of SOD and GSH were determined by biochemistry method, and Cytosolic ［Ca2+］ was determined by flow cytometry (FCM). ResultsCompared with Glu, GPcontaining serum could increase the cell survival rate and block the decrease of SOD and GSH and the increase of cytosolic［Ca2+］. Conclusion GP-containing serum can prevent the neurons from damage of glutamate oxidative neurotoxicity and the 400mg/kg/d GP-containing serum has the strongest antagonistic action.
    Establishing the hybrid cell line for HBV
    WANG Ai-hua,MA Li-xian,JIANG Yuan,SHAO Li-hua,WANG Gang,CHEN Feng-zhe,WANG Xu-ping
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(4):  374-378. 
    Abstract ( 1408 )   PDF (394KB) ( 414 )   Save
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    Objective To establish a hybrid cell different from either HepG2 cell or human primary hepatic cell which carries HBV and can be serial subcultivation in vitro. MethodsHGPRT-HepG2 cell, which was induced by 6MP, was fused with human primary hepatic cell infected by hepatitis B virus. After being screened with HAT, the hybrid cells were cloned through limiting dilution assay. Subsequently,the hybrid cells were identified by karyotype analysis. HBV DNA and HBsAg、nd HBeAg were determined at different generations. HepG2 and human primary hepatic cell infected by hepatitis B virus were taken as controls. ResultsHybrid cells were morphologically similar to HGPRT-HepG2cells and can be subcultured in vitro. The Karyotype analysis showed that the modal chromosome number was 99 in the hybrid cells, indicating that the hybrid cells contained all genomic factors from both HepG2 and human hepatic cells. HBV DNA can be detected in the cells and supernate by nest PCR. In the supernate, HBsAg and HBeAg can be detected. Conceivably the hybrid cells possessed the replication and generation in vitro of HepG2 as well as the sensitivity of human primary hepatic cell to HBV, which paved secretion of HBV DNA. Conclusion As a new hybrid cell line, it has the characteristics of both ways for further study of HBV infection cell model.
    Immunohistochemical expression of IL-8 in pulpal tissues on orthodontic rapid tooth movement through distraction osteogenesis of the periodontal ligament
    ZHANG Yong,YE Jing,GUO Xin-xing,XIAO Shui-qing
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(4):  379-381. 
    Abstract ( 1264 )   PDF (231KB) ( 284 )   Save
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    To explore the immunohistochemical expression of IL-8 in pulpal tissues on orthodontic rapid tooth movement through distraction osteogenesis. MethodsFifteen nativehealthyhybrid dogs were randomly divided into 5 groups: 3 dogs in each group. The device was activated for 2 weeks (group A), the device was fixed for 1 week (group B), the device was fixed for 2 weeks (group C), the device was fixed for 4 weeks (group D) and the control group (group E). In the experimental groups, the second premolar was pulled out, and the first premolar served as the moving tooth and the third premolar served as another tooth. Then the first premolar of the experimental animals was moved by using distraction device and then was pulled out when the device was activated for 2 weeks, fixed for 1 week, 2 weeks, and 4 weeks respectively. Then immunohistochemical expression of IL8 in pulpal tissues was determined by ELISA method. ResultsThe IL-8 concentrations in group A were significantly higher than that in the control group（P<0.05）.After the device was fixed for 1 week, the IL-8 concentrations in group B significantly decreased, and significantly decreased in group C, there were significant differences from group A（P<0.05）but no significant differences from the control group（P>0.05）. ConclusionsThe immunohistochemical expression of IL-8 is significantly increased notably after experiment, and declined to the normal level 2 weeks after the device is fixed, indicating distraction osteogenesis of periodontal ligament can increase the orthodontic tooth movement.
    Effect of the Jidu Huayu Recipe on the expression of protein C mRNA and the plasma activity of protein C in septic rats
    WU Yu-sheng,ZHANG Dao-jie,LI Jian-jun,FU Chong-jian
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(4):  382-384. 
    Abstract ( 1437 )   PDF (289KB) ( 361 )   Save
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    To explore the changes of expression of coagulation regulating protein C mRNA (PC mRNA) and activity of plasma protein C (PC) in septic rats treated with Jidu Huayu Recipe(JHR). MethodsWistar rat sepsis models were induced by cecal ligation and puncture. The Wistar rats were divided into the normal group(NP), the control group(CP), the model group(MP) and the tradicinal Chinese medicine treated group(TCMP). The expression of Protein C mRNA and the activity of plasma PC and LPS were dynamically determined on the 12h, 24h, and 48h after the sepsis operations by realtime quantitative RT-PCR and by automated coagulation analyzer and Limulus test. The effect of JHR on the survival rate was also dynamically observed. ResultsIn the course of sepsis, the expression of protein C mRNA and the activity of plasma PC in the MP group were lower than those in the CP and NP groups（P ＜0.01）, but the LPS level was increased（P＜0.01）. The expression of protein C mRNA and the activity of plasma PC in the TCMP group were higher than that in the MP group（P ＜0.01）, but the LPS level was decreased（P＜0.0 1）.　The survival rate of the septic rats treated with JHR was obviously higher than that of those without JHR treatment （P＜0.05）.　Conclusion JHR could improve the expression of protein C mRNA and the activity of plasma PC and declines the plasma LPS level of septic rats , which improves the survival rate of septic rats. This may be one of the molecule bases of JHR in treating sepsis.
    Significance of ETV6 rearrangement in acute myelogenous leukemia-M4Eo
    GAO Na,LI Zhi-hong,DING Bu-tong,CHEN Yun,WANG Yun-shan,GUO Nong-jian
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(4):  385-388. 
    Abstract ( 1761 )   PDF (314KB) ( 1950 )   Save
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    To explore the clinical significance of ETV6 gene rearrangement involved in acute myelogenous leukemia M4Eo. Methods3 cases of AML -M4Eo were prepared the chromosome after the bone marrow cells culture. R-banding technique was used to analyze the karotypes. 12p13 translocation was determined by Split-signal FISH in the interphase and metaphase cells. ETV6/ARG fusion genes were analyzed by RT-PCR. ResultsClone chromosomal abnormalities with inv(16) was found by Karotype analysis in 3 cases, abnormal fluorescence signal with 12p13 translocation was found in 1 case by split-signal FISH, and ETV6/ARG fusion gene was found by RT-PCR. This was the second report that ETV6/ARG expressed in AML－M4Eo. ConclusionThis patient is not sensitive to chemical therapy, even has worse prognosis than patient without this fusion gene. Splitsignal FISH is a reliable measure to detect ETV6 gene rearrangement.
    Long-term effect of Xuezhikang on artery elasticity in patients with myocardial infarction
    DU Xiao-zhi,LI Li,WANG Xiu-rong,JIANG Gui-hua,WANG Yuan-yuan,ZHOU Feng-hua,MENG Xiao
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(4):  389-391. 
    Abstract ( 1563 )   PDF (238KB) ( 532 )   Save
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    To investigate the longterm effect of Xuezhikang(XZK) on artery elasticity in patients with myocardial infarction. Methods Twentyfive patients with myocardial infarction longtermusing XZK were XZK group, and the other 25 patients with myocardial infarction neverusing XZK were no XZK group. 20 healthy people served as the control group. Brachial artery intimamedia thickness（IMT）, mean circumferential wall tension(MCWT), mean blood flow velocity (Vm), mean shear rate (MSR) and flowmediated brachial artery vasoactivity (FMD) were determined by highfrequency ultra sound and pulse wave velocity (PWV) was determined with Japanese Colin. VP-1000 arteriosclerosis diagnometer. ResultsCompared with no XZK group, XZK efficiently decreased the IMT, MCWT and PWV levels and significantly increased the Vm, MSR and FMD levels (P＜0.05). ConclusionsLongterm treatment of XZK can improve the peripheral arterial elasticity and prevents atherosclerosis.
    Expression of chemokine receptor CCR7 in peripheral lymphocytes of gastric carcinoma and its clinical significance
    LIU Jun,HU San-yuan,YANG Mei-xiang,GAO Wen-juan,ZHANG Yan,QU Xun
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(4):  392-394. 
    Abstract ( 1275 )   PDF (209KB) ( 433 )   Save
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    To investigate the expression of chemokine receptor CCR7 in peripheral lymphocytes of gastric carcinoma and its relationship with clinicopathological characteristics. MethodsPeripheral blood was collected from 20 healthy persons and 35 patients who underwent curative gastrectomies. The expression of CCR7 in lymphocytes was determined by flow cytometry after red cells were died and labeled with fluorescence mAb. ResultsCompared with the control group, the expression of CCR7 in the gastric carcinoma group was significantly decreased. After curative gastrectomies, the expression level of CCR7 was increased and correlated with the TNM stage (P＜0.05). ConclusionThe expression of CCR7 is downregulated in gastric carcinoma peripheral lymphocytes and relates to clinical TNM stage.
    Expression of EMA, EGP-2 and CK19 in peripheral bloods of breast cancer and its clinical significance
    SUN Shan-ping,ZANG Yi-xiu,WANG Xiao,CHEN Lian-sheng,TIAN Bin,SUN Tao,JIN Guang-chao
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(4):  395-398. 
    Abstract ( 1791 )   PDF (357KB) ( 1206 )   Save
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    To explore the expressions of EMA, EGP-2 and CK19 in peripheral cells of breast cancer and their diagnostic value as micro-metastasis targets for breast cancer. MethodsEMA, EGP-2 and CK19 were determined by flow cytometry in 65 cases of breast cancer and 37 cases of benign breast diseases， also the TNM stages, age, size of tumors, maxillary lymph nodes metastasis and expressions of estrogen receptor and progestin receptor were analyzed. ResultsThe expression rates of EMA, EGP-2 and CK19 in peripheral cells were higher than those in normal controls(P＜0.05) and closely related to the TNM stages，size of tumor and maxillary lymph node metastasis(P＜0.05). The sensitivity and accuracy of the EMA，EGP-2 and CK19 combination were higher than those of one or two (P＜0.05).
    Correlation between polymorphism of aldose reductase gene and retinopathy in type 2 diabetes mellitus
    ZHANG Ji-hui,WANG Hong-yi,LI Shu-yuan
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(4):  399-402. 
    Abstract ( 1385 )   PDF (294KB) ( 312 )   Save
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    ObjectiveTo study the relationship between polymorphism of (AC)n in 5′end of the AR gene and background diabetic retinopathy(BDR). Methods74 patients with diabetes mellitus (DM), of them, 39 with BDR and 35 without, and 40 controls were enrolled in this study. PCRagarose gel electrophoresise was used to determine the polymorphism marker of (AC)n dinucleotide of 5′end of the AR gene. Results① 7 alleles, mostly were Z, Z＋2, and Z-2 alleles were observed in type 2 diabetes mellitus and controls. ② Frequency of allele Z-2 was significantly higher in the BDR group than in the nondiabetic retinopathy(NDR) group and the control group, however, frequency of allele Z+2 was significantly lower in the BDR group than in the NDR group and the control group. Conclusion 7 alleles were found in 5′end of the AR gene in type 2 DM patients in the Shandong area, of them, Z-2 allele might be the susceptible gene and Z＋2 allel might be the protective gene.
    Comparison of 2D-and 3D-digital subtraction angiography inevaluation of intracranial aneurysms
    NING Dan,LI Cai-xia,LI Chun-hai
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(4):  403-406. 
    Abstract ( 1377 )   PDF (320KB) ( 421 )   Save
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    To evaluate the 3D-digital subtraction angiography (DSA) and 2D-DSA in diagnosis of intracranial aneurysms. Method Both 3D-DSA and 2D-DSA were performed on 53 patients with subarachnoid hemorrhage(SAH) suspected as cerebra1 aneurysms by CT. Results In the 2D-DSA group, the accurate rate, positive rate, sensitivity and specificity were respectively 84.5% (49/58), 86.8%(46/53), 92%, and 81.8%. In the 3D-DSA group, the accurate rate and positive rate were 100%(58/58) and 94.3%(50/53), and the sensitivity and specificity were both 100%. By 2D-DSA, 23 cases of neck of aneurysm were clearly showed, and 22 cases were surely displayed the source vessel, however, by 3D-DSA, 58 cases of neck of aneurysm and 56 cases of source vessels were clearly showed, the difference between 2D- and 3D-DSA was significant（P＜0.05）. The mean radiation dose and contrastmedium of 2D-DSA were (178.32±17.39) mGy and (74.57±11.95)mL, while those of 3DDSA were (99.81±9.48) mGy and (54.15±7.05) mL, the difference between the two groups was also significant（P＜0.05）. Conclusions The neck of aneurysm and source vessel are more clearly in 3DDSA than in 2D-DSA,also the radiation and contrastmedium dose are much lower in 3D-DSA than in 2D-DSA.
    P gene of hepatitis B virus in chronic hepatitis B patients who had viral-breakthrough during long-term adefovir dipivoxil monotherapy
    LIU Feng,WANG Lei,WANG Li-na,GENG Da-ying
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(4):  407-410. 
    Abstract ( 1372 )   PDF (291KB) ( 397 )   Save
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    To investigate the mutations in P gene of hepatitis B virus (HBV) in chronic hepatitis B(CHB) patients who had viral breakthrough during longterm adefovir dipivoxil monotherapy. Methods51 CHB patients who had viral breakthrough during longterm adefovir dipivoxil(ADV) monotherapy(10mg/d, duration >12 months) were enrolled in this study. Sera were collected when viral breakthrough was confirmed, and ALT, HBV DNA(real time quantitative PCR), and sequences of partial HBV P gene were determined. ResultsMutations related to ADV resistance were found in 37 of 51 CHB patients. The frequency of mutation at rt181 was significantly higher than that at rt236 (33 vs 8, P<0.001). Serum HBV DNA load in patients with combination mutation were higher than that with single mutation when the viral breakthrough occurred (6.53±0.45log10 copies/ml vs 5.37±1.08log10 copies/ml, P=0.043). ConclusionMutations in HBV P gene can be developed during ADV monotherapy in CHB patients. The HBV levels may differ in patients with different mutations.
    Expression of MMP-14 and EMMPRIN in retinoblastoma and their significance
    CHENG Yu-hong,SHEN Jia-quan,TANG Xia
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(4):  411-414. 
    Abstract ( 1087 )   PDF (395KB) ( 366 )   Save
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    To investigate the expressions of matrix metalloproteinase-14 (MMP-14) and extracellular matrix metalloproteinase inducer (EMMPRIN) in retinoblastoma(Rb) and the relationships between MMP-14 and EMMPRIN and tumor development. MethodsImmunohistochemical technique was used to determine the expressions of MMP-14 and EMMPRIN in 39 cases of paraffin embedded Rb samples. Quantitative analysis of the expressions of MMP-14 and EMMPRIN were assessed by measuring the mean gray scale of Rb tissue with LEICA IM 50 Color Pathologic Analysis System. Differences of the expressions of MMP-14 and EMMPRIN in each clinical and pathological stage were statistically analyzed, and the same step was also undertaken to study the relationship between Rb with MMP-14 positive expression and that with EMMPRIN positive expression. ResultsPositive expression rate of MMP-14 was 87.18% (34/39) and of EMMPRIN was 84.62%(33/39). The expressions of MMP-14 and EMMPRIN were significantly higher in tumors of glaucomatous stage than those in intraocular stage (P＜0.01 and P＜0.05), and the same conclusion can be concluded between those in extraocular stage and those in glaucomatous stage (P＜0.01 and P＜0.05), also the expressions of MMP-14 and EMMPRIN were significantly higher in tumors with optic nerve invasion than those without optic nerve invasion (P＜0.01 and P＜0.05). There was a statistically significant positive correlation between the expression of MMP-14 and the expression of EMMPRIN (P＜0.01). Conclusion Positive expression levels of MMP-14 and EMMPRIN may correlate with tumor invasion and metastasis and they may facilitate the Rb′ invasion and metastasis together.
    Expression of MMP-25 in the villi and decidua of early pregnancy
    WANG Li,DONG Bai-hua,SU Shi-li,ZHANG Yan,JIN Qiong,GAO Wen-juan,SHAO Qian-qian,QU Xun
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(4):  415-419. 
    Abstract ( 1402 )   PDF (428KB) ( 363 )   Save
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    To study the expression of matrix metalloproteinase-25(MMP-25) in the villi and decidua of normal early pregnant and spontaneous abortion women and to find the possible function of MMP25 in embry implantation and its relationship with spontaneous abortion. Methods Semi-quantitative RTPCR and immunohistochemistry were used to determine the expression and distribution of MMP-25 mRNA and protein in villi and decidua of normal early pregnant and spontaneous abortion women. Results① MMP-25 mRNA expressed in villi and decidua of the normal early pregnancy group and increased with the raise of pregnancy weeks in each group, also it was significantly increased compared with the abortion group, and the differences among each week and between the pregnancy group and the abortion group were significant (both P＜0.05). ② In villous tissues, MMP-25 expressed in the cytotrophoblasts and syncytiotrophoblasts and increased with the increase of pregnancy week in the normal early pregnancy group, however it only expressed in the syncytiotrophoblasts in the abortion group, and the mean ODs of the two groups were 0.21±0.02 and 0.13±0.04(P＜0.05)． In deciduous tissues, MMP-25 mainly expressed in the glandular epithelium cells in the normal early pregnancy group and was very weak in the abortion group, and the mean ODs of the two groups were 0.25±0.13 and 0.21±0.12(P＜0.05). Conclusion MMP-25 may play an important role in the embryo implantation and placentation. The decrease of MMP-25 may relate to spontaneous abortion.
    Expressions of HGF and its receptor in oral squamous cell carcinoma and neck lymph nodes
    ZHANG Yan-sheng,LIU Shao-hua,WEI Feng-cai,SUN Shan-zhen
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(4):  420-423. 
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    To explore the effect of HGF／cMet system on invasive and metastasis potential of oral squamous cell carcinoma (OSCC). MethodsFifty biopsy samples were collected from primary and relapse OSCC patients. Immunohistochemical staining was used to determine the expression of hepatocyte growth factor (HGF) and c-Met protein in OSCC tissues and neck nodes. Samples were divided into groups based on clinicopathological features and then the expression intensity was analyzed between groups. Results The expression rate of HGF and c-Met in carcinoma cells was 100％. The HGF expression rate in neck nodes was 60%. However, c-Met was not expressed in lymphocytes of neck nodes. High expression level of HGF in carcinoma cells and positive expression of c-Met in lymphocytes of neck lymph nodes significantly correlated with lymph nodes metastasis (P=0.0000), and high expression level of HGF had trends to invade (P=0.0020) and relapse(P=0.0139). Conclusion Overexpression of HGF and c-Met in OSCC and positive expression of c-Met in lymphocytes of neck nodes may induce invasion, recurrence and metastasis, predicting a poor prognosis.
    NK，IL-12 and CRP levels in pregnancy with preeclampsia
    WANG Li-jun,ZHANG Li-gong,ZUO Chang-ting
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(4):  424-426. 
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    To explore the changes of NK,IL-12 and CRP levels in pregnancy with preeclampsia and its clinical significance. MethodsThe NK cells, IL-12 and CRP levels were determined in peripheral blood of 42 pregnant women with preeclampsia, 20 normal late pregnant women and 20 normal inpregnant women. ResultsPercentage of NK cells and levels of IL-12 and CRP were higher in the preeclampsia group than in the late pregnant group and the inpreggnant group. There was a positive correlation between percentage of NK cells and level of IL-12 and no evident correlation between NK cell and CRP. ConclusionsNK cells participate in the onset of preeclampsia. IL-12 intensifies immunological rejection on maternalfetal interface by activating the NK cells and induces immunemediated damage of blood vessel endothelium and inflammatory reaction, which resulted in preeclampsia. CRP, which is increased in pregnancy with preeclampsia, may be a sensitive biochenmical marker for early diagnosing of preeclampsia.
    Capecitabine combined with late course accelerated hyperfractionatedradiotherapy for esophageal cancer
    HAN Chun-yan,SHENG Wei,HAN Jun-qing
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(4):  427-429. 
    Abstract ( 1588 )   PDF (219KB) ( 568 )   Save
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    To evaluate the response, survival and toxicity of capecitabine combined with late course accelerated hyperfractionated radiotherapy(LCAHR) for esophageal cancer. MethodsEightytwo patients were randomly divided into two groups： 41 patients in the treatment group received capecitabine combined with LCAHR, and the other 41 patients in the control group received LCAHR only. All patients were treated by conventional fractionated radiotherapy during the first twothirds of the whole course with 40?Gy in 20 fractions, followed by LCAHR with 2530?Gy in 1719 fractions, 1.5?Gy per fraction, twice a day, with an interval of more than 6 hours between fractions to a total dose of 6570?Gy. The treatment group took 1?000?mg/m2 capecitabine twice a day for fourteen days followed by a 7day rest period from the first day of radiotherapy, every 3 weeks as one cycle, totally 2 cycles. ResultsAll patients completed treatment courses. The shortterm response rate of the treatment group and the control group was 89.7% and 76.9% respectively with statistically significant differences. The 1year survival rate was 80.5% and 73.2% in the treatment and control groups respectively(P＞0.05). As for acute radiation esophagitis and myelosuppression, there were no significant differences between the two groups. ConclusionCapecitabine combined with late course accelerated hyperfractionated radiotherapy for esophageal cancer can improve the shortterm response rate. Its side effects are not statistically different from those of the LCAHR alone and are tolerable, however its longterm results need further study in clinic.
    Broken custommade tumor prosthesis with a finite element method and sleeve prosthesis for revision
    LIU Wen-guang,LI Jian-min,LIU Kai-hong,YANG Zhi-ping,LI Xin
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(4):  430-432. 
    Abstract ( 1431 )   PDF (331KB) ( 341 )   Save
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    To analyze the biomechanics characteristics of broken tumor prosthesis and optimize it with a finite element method and to evaluate the sleeve prosthesis in revision for custommade tumor prosthesis fracture. MethodsThe CT data of femur treated by software Mimics were imported into software UG2.0 to make models, and the prosthesis models were made based on the prosthesis dimension. Then the femur and prosthesis were assembled as the operations did and imported into a threedimensional finite element software ABAQUS6.5 to make a mechanics loading analysis. The sleeve revision prosthesis was designed to repair fracture of the extramedullary site of custommade tumor prosthesis with no losing. ResultsIn the broken prosthesis, conjunction of the femoral neck and the extramedullary site, conjunction of the extramedullary site and intramedullary site, one third of the intramedullary site on the exterior and interior had stress concentration, but stress of the extramedullary part was weak. Followed up for 19 months, no infection, losing, fracture of prosthesis, recurrence and metastasis, inequality of lower limb were observed. The Enneking score of preoperation was 1, of postoperation was 26, and of the last followed-up was 25. ConclusionWe should strengthen many aspects of custommade tumor prosthesis on the stress concentration site such as the diameter, shape and material. Sleeve revision prosthesis has a highly value and a good initial clinical effect in its indication.
    Initiation of callus tissues and establishment of suspension culture of Marchantia polymorpha L. cells
    YIN De-ming,WEN Xue-sen,LOU Hong-xiang
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(4):  433-437. 
    Abstract ( 1527 )   PDF (357KB) ( 569 )   Save
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    To induce callus tissues and establish suspension culture of Marchantia polymorpha L. Methods MSK-2 and MS mediums were used to induce gametophyte into callus tissues, and fuzzy comprehensive evaluation method was used to assess the optimal phytohormone combination. ResultsBoth MSK-2 and MS (2mg/L 6-BA) medium could induce callus tissue formation, and the MSK-2 medium was superior to the MS medium for callus tissue induction. Light intensity（3?0008?000 lux） effectively promoted the callus tissue growth. Optimal phytohormone combination for cell growth and secondary metabolism production was 0.5mg/L 2, 4D+2mg/L NAA+2mg/L 6BA. Conclusion Successful induction of the callus tissues and establishment of suspension culture of Marchantia polymorpha L. provide a new pathway for exploring the pharmaceutical moss to produce secondary metabolism and a new theory guidance for suspension culture of moss cell in a large scale.
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