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Initiation of callus tissues and establishment of suspension culture of Marchantia polymorpha L. cells

YIN De-ming, WEN Xue-sen, LOU Hong-xiang   

  1. Department of Natural Drug and Chemistry, School of Pharmaceutical Science, Shandong University, Jinan 250012, China
  • Received:2007-11-06 Revised:1900-01-01 Online:2008-04-16 Published:2008-04-16
  • Contact: LOU Hong-xiang

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Abstract: To induce callus tissues and establish suspension culture of Marchantia polymorpha L. Methods MSK-2 and MS mediums were used to induce gametophyte into callus tissues, and fuzzy comprehensive evaluation method was used to assess the optimal phytohormone combination. ResultsBoth MSK-2 and MS (2mg/L 6-BA) medium could induce callus tissue formation, and the MSK-2 medium was superior to the MS medium for callus tissue induction. Light intensity（3?0008?000 lux） effectively promoted the callus tissue growth. Optimal phytohormone combination for cell growth and secondary metabolism production was 0.5mg/L 2, 4D+2mg/L NAA+2mg/L 6BA. Conclusion Successful induction of the callus tissues and establishment of suspension culture of Marchantia polymorpha L. provide a new pathway for exploring the pharmaceutical moss to produce secondary metabolism and a new theory guidance for suspension culture of moss cell in a large scale.

Key words: Bryophyte, Marchantia polymorpha L., Callus tissue, Cell culture

CLC Number: 

  • Q813.1
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