关键词: ZHX2, 转录因子, 功能片段, 基因表达

Abstract:

Objective To construct a plasmid containing the functional domain of human Zinc-fingers and homeoboxs 2（ZHX2）.Methods  A truncated fragment of human ZHX2(242-501AA) was amplified with pcDNA-ZHX2-HA and cloned into pcDNA3.0 to construct expression vector pZXH2(242-501AA). The recombinant plasmids were identified with enzyme digestion and sequencing. Expression vector pZXH2 (242-501AA) was transfected into HepG2.2.15 and COS-7cells by Lipofectamine 2000, and  expression of fusion proteins was assayed by western blot. Results   Recombinant plasmids containing the functional domain (242-501AA) of the human ZHX2 gene were constructed. Enzyme digestion and sequencing analysis showed successful construction of the plasmids. Western blot showed protein expressions of the functional domain of human ZHX2 in HepG2.2.15 and COS-7cells lines. Conclusion   The functional domain of the humanZHX2 gene was successfully cloned and expressed, which sets a good basis for further study on the functional mechanism of ZHX2.