Loading...

Table of Content

      
    16 May 2009
    Volume 47 Issue 5
    Articles
    Articles
    Cloning of the human ZHX2 functional domain
    WANG Jin-Jin, SHI Wei, QI Jian-Ni, LUAN Fang, ZHAO De, GUO Chun, MA Chun-hong
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(5):  1-4. 
    Abstract ( 1185 )   PDF (729KB) ( 430 )   Save
    Related Articles | Metrics

    Objective To construct a plasmid containing the functional domain of human Zinc-fingers and homeoboxs 2（ZHX2）.Methods  A truncated fragment of human ZHX2(242-501AA) was amplified with pcDNA-ZHX2-HA and cloned into pcDNA3.0 to construct expression vector pZXH2(242-501AA). The recombinant plasmids were identified with enzyme digestion and sequencing. Expression vector pZXH2 (242-501AA) was transfected into HepG2.2.15 and COS-7cells by Lipofectamine 2000, and  expression of fusion proteins was assayed by western blot. Results   Recombinant plasmids containing the functional domain (242-501AA) of the human ZHX2 gene were constructed. Enzyme digestion and sequencing analysis showed successful construction of the plasmids. Western blot showed protein expressions of the functional domain of human ZHX2 in HepG2.2.15 and COS-7cells lines. Conclusion   The functional domain of the humanZHX2 gene was successfully cloned and expressed, which sets a good basis for further study on the functional mechanism of ZHX2.

    Effect of hypoxic training on EPO/EPOR expression in rats
    ZHAO Feng, LU Ying-Li, FENG Lian-Shi, XU Jian-Fang, ZHU Ke
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(5):  5-15. 
    Abstract ( 1340 )   PDF (2040KB) ( 247 )   Save
    Related Articles | Metrics

    Objective   To explore the effects ofdifferent hypoxia training methods on EPO-EPOR to provide a theoretical and application basis for using hypoxia training.   Methods   After adaptability training and exhaustive tests,90 rats were selected from 120 male SD rats (6week old) and randomly divided into 9 groups:the normoxia quiet control group, the continuing hypoxia group, the intermittent  hypoxia group, the normaxia training group, the living-high and training-high group(HiHi), the living-high and training-low group(HiLo), the living-low and training-high group(LoHi), the normoxia training group after HiHi, and the normoxia training group after HiLo. During a 6-week experimental period, 13.6% concentration of oxygen (equal to  an altitude of 3 500 m) was introduced into the hypoxic chamber.Rats that had undergone hypoxia training were introduced to a treadmill. The speed of training was 30m/min in hypoxia and 35m/min in normoxia. Then the rats were trained 5d/week and weresacrificed after 6 weeks. RQ-PCR, immunohistochemistry and Western blot were used to determine the mRNA and protein levels of HIF-1αin the skeletal muscle to probe further into the mechanisms of acclimatization in the skeletal muscle.   Results    Compared with the LoLo group, the mRNA level of EPO and EPOR in both the HiHi group (P＜0.01) and the HiLo group（P＜
    0.05）significantly increased. In the normoxia training group after HiHi, the mRNA level of EPO in the serum significantly increased (P＜0.05), and the mRNA level of EPO and EPOR in skeletal muscle decreased significantly; while in the normoxia training group after HiLo, the mRNA level of EPO in the skeletal muscle significantly decreased (P＜0.05).   Conclusion  It is concentration of oxygen rather than hypoxia that causes changes in the level of EPO, which may be related to exposure time of hypoxia. The mRNA level of EPO in the skeletal muscle is negatively related to that of EPO in the serum（r=-0.676）, also, the mRNA levels of EPO and EPOR in the skeletal muscle are closely related.

    Effects of grape seed proanthocyanidin extracts on advanced glycation 
    end products and expression of bone morphogenetic 
    protein7 in diabetic nephropathy rats
    LI Xian-Hua, GAO Hai-Jing, LI Bao-Ying, CHENG Mei, XU Ling, HU Zhao
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(5):  16-22. 
    Abstract ( 1185 )   PDF (1491KB) ( 433 )   Save
    Related Articles | Metrics

    Objective   To explore the effects of grape seed proanthocyanidin extracts（GSPE） on advanced glycation end products（AGEs）, expression of connective tissue growth factor（CTGF）, and bone morphogenetic protein-7（BMP-7） in diabetic rats.   Methods  45 rats subjected to injection of streptozotocin(STZ) were randomly divided into the diabetic group(DM, n=15), the lowdosage GSPE ［250mg/(kg·d)］ treatment group （T1, n=15）, and the high-dosage GSPE ［500mg/(kg·d)］ reatment group（T2, n=15）.　15 normal rats were used as the control group(C), and 15 normal rats were given 250mg/(kg·d)GSPE as the treatment control group(CT). Fasting plasma glucose(FPG), serum AGEs, systolic blood pressure, 24 h urinary protein and ratio of kidney weight to body weight were measured in each rat after 24 weeks. The renal pathological changes were examined with PAS staining and electron microscopy. mRNA and protein expressions of CTGF and BMP7 in  the kidneys were determined by RTPCR, Westernblot and immunohistochemical staining, respectively.   Results   AGEs, blood pressure, 24 h urinary protein, Ccr and ratio of kidney weight to body weight were significantly higher in[KG*3] the DM [KG*3]group [KG*3]than [KG*3]those[KG*3] in [KG*3]the C groupP＜0.01）. Renal pathology was heavily damaged in the DM group. Expressions of  mRNA  and protein of CTGF increased in the DM group compared with the C group (P＜0.01), whereas expressions of  mRNA  and protein of BMP-7 decreased (P＜0.05) in the DM group compared with the C group. Treated animals showed a reduction in serum AGEs, proteinuria and systolic blood pressure(P＜0.05 or P＜0.01).　GSPE reduced expressions of  mRNA and protein ofCTGFin the kidneys (P＜0.01),　which contributed to reversal of extracellular matrix(ECM) accumulation in diabetic nephropathy(DN). GSPE increased expressions of  mRNA  and protein of BMP-7 in the kidneys(P＜0.05 or P＜0.01).  Expressions of  mRNA and protein of CTGF, Ccr and ratio of kidney weight to body weight were significantly different between the T1 and T2 group (P＜0.05 or P＜0.01).    Conclusion  These findings suggest that GSPE holds substantial promise for the treatment of DN, and GSPE can decrease  proteinuria and attenuates the progression of nephropathy in diabetic rats. Renoprotective effects of GSPE are correlated with its suppression of AGEs, down-regulating expression of CTGF, and up-regulating expression of BMP-7.

    Effects of long-term high-protein diet on insulin secretion and plasma Ghrelin in diet-induced obese rats
    MA Li-Chuan, LI Ming-Long, CHEN Hai-Yan, ZHAO Shu-E, LIN Ai-Qing, LIN Xin-Ying
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(5):  23-26. 
    Abstract ( 1266 )   PDF (294KB) ( 452 )   Save
    Related Articles | Metrics

    Objective   To investigate the effects of long-term isocaloric high-protein diet intake on insulin secretion and plasma Ghrelin in diet-induced obese rats. 〖  Methods   Diet-induced obese rat models were established by feeding with a high fat food. The obese male rats were randomly divided to two groups: the high-protein diet group (HP, 36.7% of energy from protein, n=12) and the regular diet group (NC, 22.4% of energy from protein, n=11). The total calorie ingestion of each rat per day was similar and was maintained for 24 weeks. Body weight and fasting blood glucose were determined during the feeding period. After 24 weeks, visceral fat mass, fasting plasma Ghrelin (by enzyme-immunoassay, EIA) and fastinginsulin (by radio-immunoassay, RIA) were assayed. To evaluate the insulin secretive function of β cells, serum levels of insulin were determined in an intravenous glucose tolerance test (IVGTT).    Results  Body weight ［(490.92±39.47)g vs (545.55±31.08)g, P＜0.01］ and visceral fat mass ［(22.42±7.04)g vs (32.33±9.27)g, P＜0.05］ in HP rats were 〖KG*3]significantly lower 〖KG*3]than those in NC rats. 〖KG*3]The serum insulin levels in 5 and 10 min of IVGTT were significantly lower in HP than in NC ［(91.56±21.72)μIU/mL vs (121.29±34.03)μIU/mL, P＜0.05; (58.62±15.80)μIU/mL vs (81.12±24.36)μIU/mL, P＜0.05］. HP rats had an increased trend in plasma Ghrelin level ［(2.36±0.82)ng/mL vs (1.95±0.64)ng/mL, P=0.20］. Plasma Ghrelin was negatively correlated with body weight (r=-0.373, P＜0.05), visceral fat mass (r=-0.454,P＜0.01) and fasting insulin (r=-0.390,P＜0.05).   Conclusion  Long-term isocalorichigh-protein ingestion may reduce the first phase secretion of insulin and increase fasting plasma Ghrelin level in diet-induced obese rats. The decrease ofinsulin secretion may be secondary to Ghrelin increment and weight loss.

    Initial evaluation of the biosafty of recombinant  HBV preS2/S-AD5
    LI Zhi-Hui, YUE Ying-Ying, XING Lai-Tian, ZHANG Feng-Li, LI Yan
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(5):  27-30. 
    Abstract ( 1179 )   PDF (563KB) ( 433 )   Save
    Related Articles | Metrics

    Objective   To assess the biosafty of HBV preS2/S-AD5，a recombinant adenovirus.    Methods   The recombinant plasmid HBV preS2/S-AD5 was constructed with the first generation adenovirus vector (clontech). After digestion into linearity with PacI, preS2/S-AD5 was transfected four times into HEK-293 cells with liposome.  Four strains of replicationdefective preS2/S-AD5, named as N1, N2, N3 and N4 were obtained. HBV preS2/S-AD5 was passaged and titered in HEK-293 before inoculation of Hep-2，Hela and A549 cells and specific-pathogen-free (SPF) BALB/C mice through the nasal mucous membrane. Then the cytopathic effect (CPE) of infected cells and the general health condition of inoculated mice were observed everyday. After 10 days the important organs of the mice were taken and fixed by formalin for histopathological tests.   Results    No CPE was observed on cells which were inoculated with the first generation of N1, the 4th generation of N2, the 6th generation of N3 and the 8th generation of N4. There were no abnormities in the general health condition of the mice and no pathological changes in histological sections were observed. But there was obvious CPE on the cells inoculated with the 11th generation of N1, and mice inoculated with the 11th generation of N1 showed severe diarrhea with pathological changes in the intestines and lungs.   Conclusion   The [KG*3]recombinant [KG*3]adenovirus HBV [KG*3]preS2/S-AD5 constructed [KG*3]in our lab displays some phenomena as if it has reversely mutated to replication-competent Ads (RCAs), which can produce diarrhea and slight pneumonia in inoculated mice.

    Effect of emodin on expression of ICAM-1 in the corneas of acute keratitis
    CHEN Guo-Ling, CHEN Shuang, XIAO Ying, MAO Shu-Zhi, GAO Xue- , LIU Zhi-Yu
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(5):  31-33. 
    Abstract ( 1245 )   PDF (428KB) ( 390 )   Save
    Related Articles | Metrics

    Objective  To investigate the signification and effect of emodin on expression of intercellular adhesion molecule-1(ICAM-1) in the corneas of acute keratitis-induced by lipopolysaccharide (LPS) in Wistar rats.  Methods Pseudomonas aeruginosa LPS-induced keratitis models were established in Wistar rats. Thirty minutes before LPS exposure, emodin and its vehicle were separately injected into sub-conjunctival tissues in Wistar rats. At 1, 3, 6, 12 and 24 hours after being challenged with LPS, signs of both groups were observed, and severity of rats′ ocular inflammation was examined with a slit lamp microscope. The aqueous humor of each group was extracted and examined with hematoxylin and eosin(HE)-stained, and expression of ICAM-1 mRAN was detected by reverse transcriptase-polymerase chain reaction(RT-PCR).  Results  Ocular behavior was improved in rats which had undergone subconjunctival injection with emodin before being challenged with LPS. Inflammatory cells of the cornea and aqueous humor were less in rats of the treatment group than those of the inflammation group. The corneal edema and collagen fibers which appeared in disarrangement were also improved after emodin treatment. Expression of ICAM1 mRNA of the treatment group was increased in each point after being challenged with LPS(P<0.01), which was significantly inhibited by pretreatment with emodin（ P <0.01）.　 Conclusion Emodin can decrease expression of ICAM-1mRNA induced by LPS in the corneas of rats, and protects against acute corneal injury and improves rats′ symptoms.

    Expression of prostate apoptosis response4 in PC12 cells 
    after retonone treatment
    LIU Li, CUI Dong-Sheng, CUI Hui-Xian, GU Ping, GENG Yuan, WANG Ming-Wei
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(5):  34-37. 
    Abstract ( 1203 )   PDF (596KB) ( 305 )   Save
    Related Articles | Metrics

    Objective  To study the prostate apoptosis response-4 (Par-4) expression in PC12 cells after retonone treatment and to provide a new target for the treatment of Parkinson′s disease (PD).  Methods: 1, 5, or 10μmol/L rotenone was given to PC12 cells for 6h and 24h. MTT assay was used for determining the viability rate, and western blot was used to determine the protein level of Par-4.  Results  After rotenone treatment, cells showed different toxic appearances. After the 6h-treatment, the photodensity ratio of Par-4 to actin in the 10μmol/L group（P＝0.029 646）was significantly increased compared with the normal control group. After being pre-treated with 20μmol/L Ginestin, an inhibitor of the JNK pathway, for 1 hour, the ratio was decreased. However in the24h groups, the viability rates significantly decreased, and ratios in the 1, 5μmol/L (P=0.018461, P=0.043415) groups were increased compared with the 10μmol/L group（P=0.081 298）.  Conclusion Expression of Par-4 in PC12 cells is increased after rotenone treatment in a time and concentrationdependent manner. The JNK pathway participates in the process of Par-4 stepping up.

    Effect of Simvastatin on expression of GluR2  in the hippocampus of rats injured by Glutamate
    DING Yan, WEI Xin-Bing, HAN Yu-Xia, DING Hua
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(5):  38-41. 
    Abstract ( 1232 )   PDF (515KB) ( 332 )   Save
    Related Articles | Metrics

    Objective  To investigate the effect of Simvastatin on expression of GluR2 in the hippocampus of rats.  Methods  40 male Wistar rats were randomly divided into 4 groups: the sham-operation group, the glutamate-induced injury group,the simvastatin 10mg/kg and 20mg/kg dose groups. Normal saline or Simvastatin solution were used for intra-gastric administration for 10 continuous days. Under an anesthesia state, rats in the sham-operation group were given 4μL normal saline and those from other three groups were given 4μL glutamate solution (1mg/kg) through the lateral ventricle. The mRNA and protein expressions of GluR2 were determined 2h after injection with glutamate solution by RT-PCR or immuno chemistry.  Result  Compared with rats in the sham-operation group, both mRNA andprotein expressions of GluR2 were decreased in the hippocampus of those in the glutamate-induced injury group (P＜0.01). Simvastatin (both 10mg/kg and 20mg/kg) can significantly decrease the reduction of GluR2 induced by injection of glutamate solution (P＜0.05, P＜0.01, respectively).  Conclusion  Simvastatin can reduce or prevent glutamate-induced hippocampus injury, which may be mediated by enhancing expression of GluR2.

    Bone collagen and biomechanical propertyies in the femur of osteoporosis rats
    LIU Hai-Chun, ZHANG Jian-Feng, CHEN Yuan-zhen
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(5):  42-45. 
    Abstract ( 1236 )   PDF (716KB) ( 593 )   Save
    Related Articles | Metrics

    Objective To observe the changes of femur biomechanics in osteoporosis rats and to explore the effect of collagen content and morphology on the changes.  Methods Normal 45 nullimating female 7 month SD rats were randomly divided into three groups, A: the sham-operation group; B: the De-ovary group; C: the De-ovary and calcitonin group. Osteoporosis models were given different interventions and then were killed 20 weeks later. Biomechanical properties of the femur were determined, and  the HYP and the BMD were determined. Morphology of the femur bone trabecula and of the bone collagen were observed by HE dyeing and by masson triad color dyeing, respectively.  Results  BMD of osteoporosis rats was（0.264±0.004）g/cm2 in A group，（0.179±0.004）g/cm2　in B group，and （0.249±0.005g/cm2）in C group. HYP of rats was(0.227±0.046)mg/kg in A group， (0.194±0.032)mg/kg in B group，and (0.217±0.044)mg/kg in C group. There were evident differences in shape of collagen, bone density, bone biomechanics, content of hydroxyproline between B and C groups. There was a linear correlation between hydroxyproline and bone density and biomechanical parameters. The results showed that there was a correlation between the HYP content and postmenopausal osteoporosis, especially osteoporosis fracture.  Conclusion  There were obvious changes in femur biomechanics and collagen. Changes of bone collagen are the main reason of biomechanical property changing. Enhancing collagen of the bone can improve biomechanical properties of the bone and decrease the osteoporosis fracture rate.

    AMP-activated protein kinase in high glucose-induced  proliferation of glomerular mesangial cells
    XU Jing, LIU Yi, WAN Qiang, JIA Zhen-Hua, WANG Rong
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(5):  46-49. 
    Abstract ( 1237 )   PDF (429KB) ( 404 )   Save
    Related Articles | Metrics

    Objective To investigate the expression and activity of AMPK in high glucose-induced proliferation of glomerular mesangial cells（GMCs）.
     Methods GMCs were incubated in four groups for 24 hours: the control group(glucose 5.6mmol/L), the high glucose group（glucose 22.0mmol/L）， the low AICAR group（0.5mmol/L AICAR＋22.0mmol/L glucose）， and the high AICAR group（1.0mmol/L AICAR＋22.0mmol/L glucose）. The proliferation of GMCs was determined by MTT and the cell cycle of GMCs by flow cytometry; AMPKα1 and α2 mRNA levels were observed by RT-PCR;and protein levels of AMPKα and P-AMPKα were determined by Western blot.  Results Compared with the control group, high glucose induced proliferation and DNA synthesis of GMCs with impaired AMPKα1and α2 mRNA levels and impaired protein levels of AMPKα and P- AMPKα; AICAR(activator of AMPK) suppressed high glucose-induced cell proliferation and DNA synthesis while enhancing the AMPKα1 and α2mRNA and protein levels of AMPKα and P-AMPKα.  Conclusion  High glucose can induce proliferation and DNA synthesis of GMCs, which may correlate with impaired AMPKα1 and α2 mRNA levels and impaired protein levels of AMPKα and P-AMPKα.

    Construction of nervous specific promoter PDGF-EGFPvector and  identification of tissue specificity
    REN Kai, DING Yan, CUI Yun-Yan, YANG Ling-Ling, ZHANG Jing, CUI Xing
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(5):  50-53. 
    Abstract ( 1192 )   PDF (1012KB) ( 305 )   Save
    Related Articles | Metrics

    Objective To construct an expression vector of enhanced green fluorescent protein (EGFP) containing nervous tissue-specific promoter-PDGF.  Methods  The PDGF-promoter sequence was amplified by PCR and the template was the genome of human blood cells. The PCR products were cloned into the pEGFP-1 vector and then pP DGF-EGFP vector was constructed. The vector was transfected into human neuroblastoma cells, SK-N-SH and four kinds of non-nervous recourse cells by lipofectamine. Expression of EGFP of transfected cells and tissue specificity of the vector were determined by transfection.  Results The vector of pPDGF-EGFP was successfully constructed. After a transfection experiment, the EGFP was highly expressed in SK-N-SH cells and scarcely expressed in the other four cells.  Conclusion  The vector of pPDGF-EGFP has a high nervous tissue specificity. It lays a foundation for studying target gene therapy of nervous system diseases.

    Effect of the Yiqi Huoxue decoction on CTGF and PDGF-B in rats with chronic renal failure
    YU Ying, SI Guo-Min, ZHAO Bao
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(5):  54-58. 
    Abstract ( 1013 )   PDF (1161KB) ( 408 )   Save
    Related Articles | Metrics

    Objective To investigate the effect of the Yiqi Huoxue decoction on CTGF and PDGF-B of chronic renal failure(CRF) rats．  Methods Rat models of CRF were established by feeding Adenine．All the rats were randomly divided into six groups：the normal group， the model group， the high dosage Yiqi Huoxue decoction group， the medium dosage Yiqi Huoxue decoction group，  the low dosage Yiqi Huoxue decoction group and the losartan group． After 8 weeks，  levels of serum creatinine(SCr)，  blood urea nitrogen(BUN)，  triglyceride(TG)，  cholesterol(CHO)， plasma albumin(ALB)， and hemoglobin(HB) were determined． In addition， the results of pathology were found by HE and the expressions of CTGF and PDGF-B were determined by the immuohistochemical method．   Results   Compared with the model group， levels ofSCr,  BUN,  and CHO in the Yiqi Huoxue decoction groups and the losartan group were decreased,  while levels of ALB and HB were increased (P<0.05)．Pathological results of the Yiqi Huoxue decoction groups and the losartan group were betterthan those of the model group， and those of the high dosage Yiqi Huoxue decoction group were the best． Expressions of CTGF and PDGF-B in the Yiqi Huoxue decoction groups and the losartan group were decreased compared with the model group.  Conclusions   Yiqi Huoxue decoction can efficiently decrease SCr， BUN， and CHO levels and increase ALB and HB levels， reduce pathological injury and the CTGF and  PDGF-B expressions， and postpone the aggravation of renal function．

    Effect of recombinant human growth hormone on intestinal mucosal cellular apoptosis in severely scalded rats
    SHI Wen, XIN Nai-Jun, MA Yin-Dong, YANG Tao, JIA Jun, JIANG Jin-Zhu, GUO Cheng-Hao, SONG Guo-Dong
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(5):  59-62. 
    Abstract ( 1014 )   PDF (445KB) ( 246 )   Save
    Related Articles | Metrics

    Abstract: Objective  To investigate whether recombinant human growth hormone (rhGH) can improve the intestinal mucosal barrier in severely scalded rats and by which possible mechanisms these changes occur.  Methods  42 adult male Wistar rats were randomly divided into 3 groups: the control（n=6）, scald(n=18) and GH groups(n=18). Rats in the scald and GH groups were inflicted with 25% total body surface area full-thickness skin scald on the back and immediately followed by intraperitoneal injection of dexamethasone (80mg/kg). The scalded rats in the two groups were subcutaneously injected with normal saline and rhGH ［1.33U/（kg·d）］   respectively from 2 hours to 3 days after scald. At 12, 24 and 72h after scald, the intestinal mucosal histomorphology in distal ileum tissues, morphological changes of apoptotic cells and apoptotic rate and changes of activity of Caspase-3of intestinal mucosal cells were observed by HE stain, TUNEL and fluorescence method, respectively.  Results Intestinal mucosal histomorphology and intestinal epithelia in the scald group was severely injured but significantly melioratedby rhGH to near that in the control group. The intestinal mucosal cellular apoptotic rate in both scald and GH groups was significantly higher than that in thecontrol group(P<0.01) while that in the GH group was evidently lower than that in the scald group (P<0.01),and reached a peak （15.40%±3.21% vs 38.80%±6.98%） at 24h after burn. Also, activity of Caspase-3 was more significantly increased in the scald and GH groups than in the control group(P<0.05) while it was lower in the GH group than in the scald group(P<0.05), and reached a peak （156.13±4.66U/mg vs 214.60±9.29U/mg） at 24h.   Conclusion   rhGH administered inearly severe burn could inhibit intestinal mucosal cellular apoptosis, which might be induced by down-regulation of activity of Caspase-3. As a result, intestinal mucosal integrity and function was maintained.

    Antioxidant ability of type I and type II endometrial cancer cells
    DING Ning, BI Wen-Xiang, KONG Feng, ZHANG Pei-Hai
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(5):  63-66. 
    Abstract ( 1276 )   PDF (259KB) ( 308 )   Save
    Related Articles | Metrics

    Objective To compare the ability of type Ⅰand type Ⅱ endometrium cancer cells for resisting oxidative stress and investigate its related mechanism Method  ①  TypeⅠand type Ⅱ endometrium cancer cells were challenged by H2O2, and cell proliferation was determined by the MTT assay. ② TypeⅠor type Ⅱ endometrium cancer cells were treated with 120μmol H2O2 or 800?μmol H2O2, and cellular ROS, GSH, and GSSG levels and SOD, CAT and GPx activities were determined.  Results Without H2O2, typeⅠ endometriumcancer cells, compared with the type Ⅱ endometrium cancer cells, had a lower ROS level and CAT activity and a higher GSH level, GSH/GSSG ratio, and SOD and GPx activities. Under oxidative stress, the ROS level increased in both typeⅠand type Ⅱ endometrium cancer cells. GSH level, GSH/GSSG ratio, and SOD and CAT activities were decreased in type Ⅰ endometrium cancer cells while GSH and GSSG levels, GSH/GSSG ratio, and SOD, CAT and GPx activities increased in type Ⅱ endometrium cancer cells.  Conclusion  Oxidative stress is higher in typeⅡendometrium cancer cells than in typeⅠendometrium cancer cells. The oxidationreductions ystem and anti-oxidase system in type Ⅱ endometrium cancer cells can effectively respond to oxidative stress, and cells can effectively resist oxidative stress.

    Non-cell corynebacterium parvum product combined with slow intra-tumorr  elease of drugs inhibits B16 melanoma in mice
    GUO Chun, WUN Na, HONG Hai-Jie, WU Wen-Liang, HE Jing, YU Bao-Fa, GAO Shangxian, HU Bao-Fang
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(5):  67-70. 
    Abstract ( 1268 )   PDF (814KB) ( 321 )   Save
    Related Articles | Metrics

    Objective  To explore the effects of non-cell corynebacterium parvum product（NCPP） combined with slow intra-tumor release of drugs on B16 melanoma in mice．  Methods  Tumor-bearing mice were randomly divided intofour groups： the normal saline group， the slow intra-tumor release drug group, the NCPP group and the drug combination group． Seven days later， mice were treated again and the gross tumor volume was determined． Then 2 weeks later， all the tumor-bearing mice were sacrificed to measure tumor weight. Pathologic changes of tumors were observed under light microscopy. Infiltrations of T cells were determined in the splenic organs．  Results   Drug combination can effectively contain the tumor growth (P＜0．05). HE staining results showed that there was a large area necrotic tissue in the tumor(P＜0．05)，　and CD8+ T cells were significantly increased in the drug combination group(P＜0．01)．  Conclusion NCPP combined with slow intra-tumor release drugs is one of the effective management method for directly inhibiting tumor growth．

    Mechanism of mesenchymal stem cells in treatment of brain damage caused by carbon monoxide poisoning (COP) in rats
    CHU Hai-Ping, LIU Zhi-Yu, SUN Jin-Hao, HE Yu-Xiang
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(5):  71-74. 
    Abstract ( 1118 )   PDF (647KB) ( 311 )   Save
    Related Articles | Metrics

     Objective  To investigate the effect and mechanism of mesenchymal stem cells (MSCs) transplanted for brain damage induced by carbon monoxide poisoning (COP) in rats.  Methods  MSCs were isolated from the bone marrow of Wistar rats, cultured and generated in vitro and then made into a suspension. The rat carbon monoxide poisoning models were established by peritoneal injection of 100-150mL/kg CO. MSCs marked with Bromodeoxyuridine(Brdu) were transferred into rats through the internal carotid artery. The Morris water labyrinth experiment was used to examine the nervous system function of the model rats. Damage area cells in the cornu ammonis were determined with an immunohistochemical method and Nissl'sstaining.  Results   MSCs can survive and reduce apoptosis of nerve cells. One and four weeks later, Morris water labyrinth experiments demonstrated that the rats′ space learning capability greatly improved and pathological changes of the brain organization were obviously reduced(P＜0.05).   Conclusion   Mesenchymal stem cells (MSCs) transplantation is effect in treating brain damage induced by carbon monoxide poisoning (COP) in rats.

    Effect of glucose and insulin on visfatin expression in 3T3-L1 adipocytes
    WANG Kun, WANG Wen-Hui, CHEN Miao, CHEN Fu-Lian, LOU Ning
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(5):  75-77. 
    Abstract ( 1175 )   PDF (504KB) ( 184 )   Save
    Related Articles | Metrics

    Objective  To investigate the effects of different concentrations of glucose and insulin on expressions of visfatin mRNA and protein in 3T3-L1 adipocytes and to explore the role of visfatin on diabetes mellitus.  Methods 3T3-L1 pre-adipocytes were cultured in vitro and differentiated. Mature adipocytes were incubated with different concentrations of glucose and insulin for 48 hours. Then total cellular RNA and protein were extracted, and expressions of visfatin mRNA and protein were determined by semi-quantitative RT-PCR and western blot with β-actin as an internal control.  Results  Compared with the control group, expressions of visfatin mRNA and protein in mature adipocytes were decreased by high glucose（P＜0.05）, but were not significantly changed by different concentrations of insulin（P＞0.05）.  Conclusion   In in vitro studies, a high level of glucose can down-regulate visfatin expression, but concentration of insulin has no effect on visfatin expression.

    Expression of MMP-9 and regulation of neuregulin on the brain of rats following ischemia/reperfusion
    WANG Hai-Ping, LI Zeng-Wei, CHI Zhao-Fu
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(5):  78-82. 
    Abstract ( 1260 )   PDF (534KB) ( 393 )   Save
    Related Articles | Metrics

    Objective   To explore expression of matrix metalloproteinase-9 (MMP-9) and effect of neuregulin-1β (NRG-1β) on brain tissues of rats following cerebral ischemia/reperfusion.   Methods   Animal models of middle cerebral artery occlusion/reperfusion (MCAO/R) were established by a filament method in the left external-internal carotid artery in 100adult healthy male Wister rats. Rats in the treatment group were injected with 1.5％NRG-1β 5μL in the internal carotid artery(ICA). Expression of MMP-9 was determined by immunohistochemical and immumofluorescent staining and western-blotting assay. Results  Hypoxia and ischemia can cause expression of MMP-9. With the prolongation of the ischemic time, an increased expression of MMP-9 was obtained in the control group. Expression of MMP-9 was decreased in the NRG-1β treatment group compared with the control group at the same time point and the corresponding region（P＜0.01）.   Conclusion  MMP-9 might contribute to inflammatory reaction after ischemia-reperfusion cerebral insult by different methods. NRG-1β might regulate the activation of MMP-9, disturb the development of inflammatory reaction, improve the micro-environment of neuron survival, delay the course and degree of the neuron by various methods, and further play a neuro-protective role in ischemia cerebral insult.

    Application of contrast-enhanced MDCT in combination with FDG PET/CT in esophageal cancer staging
    HUANG Shao-Qi, HOU Zhong-Yu, TAN Ru, GE Quan-Xu, YAO Shu-Zhan, LIU Qing-Wei
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(5):  83. 
    Abstract ( 1286 )   PDF (434KB) ( 357 )   Save
    Related Articles | Metrics

    Objective  To investigate the clinical effect of contrast-enhanced multiple-detector spiral computed tomography (MDCT) in combination with FDG PET/CT on esophageal cancer staging.  Methods  MDCT and FDG PET/CT were performed and prospectively interpreted in 79 patients with newly diagnosed esophageal cancer within one week. Primary lesions，invasion of adjacent structures, locations and numbers of metastases to the individual lymph node groups were observed. All the lesions were histopathologically confirmed. Results of MDCT, PET/CT and their combination were compared with histopathological results.  Results  Lesion detective rate was 78.3% by MDCT, 96.4%by PET/CT, and 96.4% by their combination. Sensitivity, specificity and accuracy for T4 staging tumors was 70.0%, 94.5% and 91.6% by MDCT, 90%, 68.5% and 71.1% by PET/CT, 90%, 98.6% and 97.6% by their combination. Differences in specificity(P=0.0) andaccuracy(P=0.0) between PET/CT alone and MDCT in combination with PET/CT were statistically  significant. For detecting lymph nodes metastases, the sensitivity, specificity and accuracy by MDCT was 70.5%, 95.9%, and 90.1%, and by FDG PET/CT was 86.1％, 97.1%, and 94.6%,whereas by their combination was 94.3%, 98.6%, and 97.6%. Differences in sensitivity （P=0.0）, specificity（P=0.007） and accuracy（P=0.0）between MDCT alone and MDCT in combination with PET/CT were significant, also differences in sensitivity（P=0.021） and accuracy（P=0.004）between PET/CT alone and MDCT in combination with PET/CT were obviously significant.  Conclusion Diagnosis and staging of oesophageal cancer can be improved by using both FDG PET/CT and MDCT at the same time.

    Expression of NKG2D on NK cells in intracranial tumors
    MA Li-Xin, LI Gang, SU Yu-Hang, ZHANG Cai, ZHANG Jian
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(5):  88-91. 
    Abstract ( 1230 )   PDF (685KB) ( 198 )   Save
    Related Articles | Metrics

    Objective  To explore expression levels of NKG2D on NK cells in patients suffering from pituitary adenoma, glioma, meningioma, and craniopharyngioma to obtain the relation ship between NKG2D-expressing NK cells and intracranial tumors.  Methods  Blood samples were lysed by FACS lysing solution, and expression of NKG2D on NK cells was determined by flow cytometry.   Results  The results revealed that expression of NKG2D was not obviously changed in glioma, meningioma, and craniopharyngioma but was decreased in pituitary adenoma. There were relations hips between the NKG2D expression and defect of visual field in pituitary adenoma.  Conclusion In intracranial diseases, expression of NKG2D had no changes in glioma, meningioma, craniopharyngioma and was obviously changed in pituitary adenoma.NKG2D expression is related to the progression of pituitary adenoma.

    Expression of programmed cell death factor 10 protein and its clinicopathological significance in gastric cancer
    ZHU Xiao-Yan, WANG De-Rong, QU Fei
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(5):  92. 
    Abstract ( 961 )   PDF (398KB) ( 302 )   Save
    Related Articles | Metrics

    Objective  To investigate expression of programmed cell death factor 10 (PDCD10) protein and its clinicopathological significance in the progress of gastric cancer.  Methods Expression of PDCD10 was determined by the SP immunohistochemical method in 26 cases of normal gastric mucosa, 22 cases of chronic atrophic gastritis with intestinal metaplasia and 66 cases of gastric carcinoma. Relationship between expression of PDCD10 and clinicopathological characters of gastric carcinoma was analyzed.  Results  The positive rates of expression in normal gastric mucosa, chronic atrophic gastritis with intestinal metaplasia and gastric carcinoma were 11.5%, 31.8% and 63.6% respectively，which was progressively upregulated. In gastric cancer, expression was not related to sex, age, and site of tumors (P＞0.05), in contrast, positive rates were related to the degree of differentiation clinical stages, lymph nodes and distant metastases (P＜0.05).  Conclusion   PDCD10 expression in gastric cancer tissues is significantly higher than that in the normal gastric mucosa and chronic atrophic gastritis with intestinal metaplasia tissues. There is a correlation between expression of PDCD10 and histological grades and metastases of gastric cancer.

    Expression and significance of PTEN and p-Akt in infantile hemangioma
    ZHAO Yang, LV Ren-Rong, HUO Ran, GAO Feng-
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(5):  95-98. 
    Abstract ( 1147 )   PDF (785KB) ( 424 )   Save
    Related Articles | Metrics

    Objective  To determine expressions of PTEN and p-Akt in different stages of infantile hemangioma, and to explore the correlation between PTEN and p-Akt and approach significance of PTEN and p-Akt in the occurrence and development of infantile hemangioma.  Methods  Expressions of PTEN and pAkt were determined by SP immuno histochemical technique in cases of proliferating hemangiomas, involuting hemangiomas, vascular malformations, and normal skins. Mean optical density was measured by an image analysis system.  Results  Expression of PTEN in proliferating hemangiomas was lower than in the other groups (P<0.01). Expression of p-Akt in proliferating hemangiomas was higher than in the other groups (P<0.05). No statistical difference was found in expressions of PTEN and p-Akt among involuting hemangioma, vascular malformation and normal skins. Expression of PTEN was negatively correlated with expression of p-Akt (r=－0.719?2,P＜0.05).  Conclusion  PTEN and p-Akt may play important roles in the proliferating phase of infantile hemangioma.

    Continuous ultrafiltration during cardiopulmonary bypass in infants
    ZHANG Ai-Hua, YANG Da-Kuan
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(5):  99-101. 
    Abstract ( 1050 )   PDF (262KB) ( 259 )   Save
    Related Articles | Metrics

    Objective  To investigate the clinical effect of continuous ultrafiltration in open heart surgery with cardiopulmonary bypass (CPB) for infants. 
     Methods  Fifty patients with congenital heart defects were randomly divided into two groups. In the control group, conventional CPB was used without ultrafiltration.In the treatment group, in addition to the same CPB, continuous ultrafiltration was used throughout CPB. Serum concentrations of interleukin-6(IL-6) and tumor necrosis factor(TNF), hematocrit(HCT), serum albumin concentrations, pulmonary function and the recovery time after the operations were measured.    Results  Serum concentrations of IL-6 and TNF were significantly decreased, HCT and serum albumin concentrations were significantly increased, pulmonary function was improved and the recovery time after the operations was shortened in the treatment group compared with the control group (P<0.05).  Conclusion Continuous ultrafiltration can effectively exclude some harmful inflammatory mediators, concentrates the blood, attenuates lung edema and injuries, and promotes recovery after operations.

    Pathogenesis probe of embolism in the portal vein system
    DING Xia, ZHAO Bin, MA Zi-Tang, JIAShou-Qin
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(5):  106-108. 
    Abstract ( 1271 )   PDF (257KB) ( 286 )   Save
    Related Articles | Metrics

    Objective To analyze the location characteristics of embolism in the portal vein system and to probe the pathogenesis of this disease.  Methods  267 cases of embolism in the portal vein system were collected and were given at least one of the following examinations: ultrasound, MRI, or DSA, and all were givenplain and enhanced scanning of multi-spiral CT. 242 of them had malignant tumors proved by operation or biopsy. Involved condition of both the upper mesenteric veins and splenic veins were compared, and involved scale of embolism in the portalvein system was detected.  Results ① Upper mesenteric veins （145/267，79.2%）were more likely to be involved than splenic veins （17/267，6.4%）in embolism ofthe portal vein system（P<0.05）. ② The total number of involved vein sections in the portal vein system of this disease was obviously higher in the noncancerous group than in the malignant tumor group(P＜0.05).  Conclusion  Functional， anatomical and blood dynamic characteristics of the mesenteric vein are greatly related to embolism of the portal vein system. Embolism scale caused by noncancerous diseases is wider than that caused by malignant tumors.

    Application of multi-slice helical CT and image post-progressing in larynx and hypopharynx carcinoma
    CHAI Xiang-Jun, PANXin-Liang, LEI Da-Peng, ZHANG Li-Qiang
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(5):  109-112. 
    Abstract ( 1148 )   PDF (355KB) ( 350 )   Save
    Related Articles | Metrics

    Objective To evaluate the clinical application of helical CT in larynx and hypopharynx carcinoma.  Methods  45 patients with larynx orhypopharynx carcinoma were confirmed by fibrolaryngoscope and histopathology. All were subjected to CT scanning，  MPR, CTVE, and transparent 3D VRT images were reconstructed before the operations.  Results There were no significantly  differences between transection image and histopathology（Q=0.68，P＞0.05）, between MPR and histopathology（Q=0.34，P＞0.05）,　and between CTVE and fibro laryngoscope（Q=0.59，P＞0.05）in diagnosis of larynx and hypopharynx carcinoma. However, the fibrolaryngoscope was better than CTVE or VRT in demonstrating membrane carcinoma of the larynx andhypopharynx（Q=5.60，P＜0.01）.   Conclusion  Transection image in combination with MPR, CTVE,and VRT images could improve the diagnostic value of multi-slice helical CT in larynx and hypopharynx carcinoma.

    Expressions of p-STAT3， MMP-2 and VEGF in retinoblastoma
    YANG Le, SHEN Jia-Quan, CHENG Yu-Hong
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(5):  113-116. 
    Abstract ( 1153 )   PDF (460KB) ( 164 )   Save
    Related Articles | Metrics

    Objective  To investigate the expression of signal transduction and activators of transcription-3(p-STAT3), matrix metalloproteinase-2 (MMP-2) andvascular endothelial growth factor(VEGF)in retinoblastoma(RB) and the relationships  among p-STAT3, MMP-2﹑VEGF and tumor development.  Methods  Immunohistochemical technique was used to detect expressions of p-STAT3, MMP-2 and VEGF in 39 cases of paraffin embedded RB samples. Quantitative analysis of expressions of p-STAT3,MMP-2 and VEGF were assessed by measuring the mean gray scale of RB tissues with LEICA IM 50 Color Pathologic Analysis System. Differences of expressions of p-STAT3,MMP-2 and VEGF in each clinical and pathological stage were statistically analyzed.  Results The positive expression rate of p-STAT3 was 66.7%（26/39）, of MMP-2 was 79.5 %（31/39）and of VEGF was 64.1 %（25/39）. There was a statistically significant positive correlation between expressions of p-STAT3，　MMP2 and VEGF with optic nerve invasion（P＜0.05,P＜0.01,P＜0.01）, also there was a statistically significant negative correlation between expressions of p-STAT3，　MMP2 and VEGF with differentiation of cancers（P＜0.01,P＜0.01,P＜0.05）, but there was no correlation with age, gender and diseased eyes of patients with RB. Also, there was astatistically significant positive correlation between expression of p-STAT3 and expression of MMP-2 (P＜0.01) and between expression of p-STAT3 and expression of VEGF (P＜0.01).  Conclusion The positive expression levels of p-STAT3, MMP-2 and VEGF may correlate with tumor invasion and metastasis and they may promote invasion and metastasis of RB.

    Vasculogenic mimicry in malignant gastric stromal tumors
    GU Xiao-Meng, YU Chao
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(5):  117. 
    Abstract ( 1078 )   PDF (368KB) ( 334 )   Save
    Related Articles | Metrics

    Objective  To investigate whether malignant gastric stromal tumors contain PAS-positive patterned matrix-associated  vascular channels, that is vasculogenic mimicry(VM) independent of tumor angiogenesis.  Methods 36 tissue samples were divided into three groups: the poorly, the moderately and the highly malignant gastric stromal tumors. Tissue sections were stained with VEGF, CD31 1and periodic acid Schiff (PAS). The number of VEGF, microvascular density (MVD) or vasculogenic mimicry density (VMD) was calculated.  Results PAS-positive patterned matrix-associated vascular channels were detected in 30.56%(11/36) of malignant gastric stromal tumors. The number of VEGF or MVD in tumors containing patterned channels was fewer than that in tumors not containing patterned channels,and there was a significant difference between them(P<0.05). Also, the malignant degree of gastric stromal tumors, and proportion of malignant gastric stromal tumors containing patterned channels and quantity of VMD were increased.  Conclusion  In malignant gastric stromal tumors, there is the VM mechanism underlying some tumor cells deforming themselves and secreting an extracelluar metrix, thus the PAS-positive patterned matrix-associated vascular channels appear and bring a blood supply for tumors to sustain growth and metastasis.

    Synthesis and characterization of biotinylated chitosan material
    YAN Yong-Bo, WANG Zhong-Yan, LI Liu-Fa, HAN Feng, XU Kai, MO Feng-Kui
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(5):  120-124. 
    Abstract ( 1189 )   PDF (1461KB) ( 550 )   Save
    Related Articles | Metrics

    Objectives To synthesize and characterize biotinylated chitosan.  Methods  A new targeting carried material (biotinylated chitosan) was synthesized by reaction of the amino group of chitosan and carboxy group of biotin under 4-Dimethylaminopyridine and N, N′-dicyclohexylcarbodiimide catalyst conditions after being depurated by  dialysis methods and was lyophilized. The chemical structure of the synthesized biotinylated chitosan was characterized by the Fourier transform ation  infrared spectrometer (FTIR), nuclear magnetic resonance (1HNMR), and differential scanning calorimeter (DSC) methods  Results A white power solid biotinylated chitosan was obtained after refinement, and figures of its FTIR,1HNMR and DSC were different from its monomer. The substitution value was 42.28% and the yield was14.95%.  Conclusion  Biotin and chitosan are chemically bonded through an amide linkage so that the Biotinylated chitosan is successfully synthesized with simpleprocesses and low cost.

    Defining fusion peptide on glycoprotein of the Hantavirus GM04-38
    CAO Hai-Xia, TAO Ze-Xin, ZHENG Xiao-Min, LIU Xiao-Li, WANG Gui-Ting, HU Hong-Zhi, WEN Hong-Ling, SONG Yan-Yan, ZHAO Li, YAO Ping, WANG Zhi-Yu
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2009, 47(5):  125-130. 
    Abstract ( 1283 )   PDF (1010KB) ( 181 )   Save
    Related Articles | Metrics

     Objective  To determine the potential putative fusion region on glycoprotein of the Hantavirus GM04-38.  Methods Site-directed mutagenesis was used to construct 10 GP gene mutants, which were then expressed in Vero E6 cells. IFA was performed to determine expression of GPs genes, and Giemsa staining was used to observe cell fusion activities.  Results  IFA showed that the GPs genes were successfully expressed, and the fluorescent signal was robust and concentrated in the perinuclear region of the transfected cells. But cell fusion activities were not found after mutation.   Conclusion   Mutation of 10 amino acids can obviously influence cell fusion activities, suggesting that it is possible that fusion peptide is in this region.

Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd