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Expression of LMP7 in alcoholic liver disease under carbonyl stress

ZHANG Li1，YAN Ming1，ZHU Ping1，WANG Yan-mei2，FENG Jin-bo3   

  1. 1. Department of Gastroenterology, Qilu Hospital of Shandong University；2. Institute of Epidemiology and Biostatistics, School of Public Health, Shandong University
  • Received:2006-12-30 Revised:1900-01-01 Online:2007-08-24 Published:2007-08-24
  • Contact: YAN Ming

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Abstract: Objective: To evaluate the expression of proteasome LMP7mRNA in rat models of alcoholic liver disease under carbonyl stress and investigate the effect of LMP7 in the pathogenesis of alcoholic liver disease. Methods: Rat models of alcoholic liver disease were establish by the gastric perfusion method and killed at 6,8,10 and 12 weeks. Protein carbonyl contents at different stages were determined by 2, 4-Dinitrophenylhydrazine colorimetry, and expressions of mRNA for LMP7 were determined by RT-PCR. Results： Compared with the control group, contents of protein carbonyl were much increased in the model groups in a dose-dependent manner (P＜0.05). Expressions of mRNA for LMP7 were decreased in the model groups, which negatively correlated with the protein carbonyl content. Conclusion： Hepatic tissues are under carbonyl stress in alcoholic liver disease, and the alcohol administration results in a decreased expression of LMP7, which may play a vital role in the pathogenesis of alcoholic liver disease.

Key words: Liver disease, alcoholic, Low molecular weight polypeptide7, Carbonyl stress, Polymerase chain reaction, Fluorogenic quantitative

CLC Number: 

  • R575
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