Journal of Shandong University (Health Sciences) ›› 2020, Vol. 58 ›› Issue (5): 46-50.doi: 10.6040/j.issn.1671-7554.0.2020.484

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Application value of chemiluminescent immunoassay in the detection of 2019-nCoV antibodies

LI Xiaofei, LIU Guiming, LI Wanlan, LYU Songqin, NIE Lei, ZHANG Juan, XU Liping, LU Nihong   

  1. Department of Laboratory, Kunming Third Peoples Hospital, Kunming 650041, Yunnan, China
  • Published:2022-09-27

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Abstract: Objective To evaluate the application value of chemiluminescent immunoassay in the detection of 2019-nCoV antibodies. Methods The sample results from 18 confirmed COVID-19 cases detected by chemiluminescent immunoassay and colloidal gold kit were compared, and 2 samples were randomly selected and tested with the above two methods after gradient dilution. The blood samples of 39 clinically diagnosed COVID-19 cases and 90 healthy controls were detected with chemiluminescent immunoassay, and the detection rates of antibodies in different stages of disease were recorded. Results Chemiluminescent immunoassay and colloidal gold method showed all of 18 patients’ samples were positive. After 625 times dilution, the 2 samples were still positive by chemiluminescent immunoassay, but the maximum dilution rate by colloidal gold reagent was 25 times. The sensitivity and specificity of chemiluminescent immunoassay for COVID-19 were 82.05% and 100%, respectively, which were consistent with the clinical diagnosis(kappa value was 0.864). The detection rate of antibodies in serum samples by chemiluminescent immunoassay increased with the time of onset. Conclusion Chemiluminescent immunoassay has higher sensitivity than colloidal gold method in the detection of 2019-nCoV antibodies, which can be used for rapid screening of a large number of samples. Chemiluminescent immunoassay can serve as a supplementary detection method of 2019-nCoV.

Key words: Novel coronavirus, Chemiluminescent immunoassay, Colloidal gold method, Specific antibody

CLC Number: 

  • R446.6
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