Journal of Shandong University (Health Sciences) ›› 2018, Vol. 56 ›› Issue (7): 21-27.doi: 10.6040/j.issn.1671-7554.0.2017.493

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Protective effects of DRSAb on ischemia reperfusion induced renal injury in rats

SUN Jingjing, ZHANG Jiangwei, KUANG Peidan, ZHANG Ying, XUE Wujun, ZHENG Jin   

  1. Department of Kidney Transplant, First Affiliated Hospital of Xian Jiaotong University, Xian 710061, Shaanxi, China
  • Published:2022-09-27

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Abstract: Objective To explore the protective effects of Na+/K+-ATPase DR region specific antibody(DRSAb)on ischemia/reperfusion induced renal injury and its mechanism. Methods Spragur-Dawley(SD)male rats were immunized with synthesised DR region peptides to prepare DRSAb. The biological activity of DRSAb was detected with Western blotting, and the mechanism of cell signal transduction was determined with flow cytometry. The activity of HK-2 cells was assessed with MTT. The protective effect of DRSAb on ischemia-reperfusion injury in rat kidney was evaluated with animal models of ischemia-reperfusion injury. Results The results showed that 90.02% of HK-2 cells could bind with DRSAb. The DRSAb immune sera could significantly enchance the tolerance of HK-2 cells to hypoxia, compared with control seara(OD: 0.50±0.03 vs 0.10±0.02, P<0.001). DRSAb could activate PI3K/AKT and PKCε kinase(P<0.001), which could be inhibited by LY294002 and PEAVSLKPT(P<0.001). Animal experiments indicated that DRSAb group demonstrated a significant improvement in renal function with a lower serum creatinine(sCr)and blood urea nitrogen(BUN)levels 3-6 days postoperative compared with the Control group(P<0.001). Histological evaluation showed that the Control group had visible vascular congestion, epithelial cell swelling and extensive tubular necrosis. 山 东 大 学 学 报 (医 学 版)56卷7期 -孙晶晶,等. DRSAb对缺血再灌注损伤大鼠肾脏的保护作用 \=-However, DRSAb group only had slight vascular congestion, mild epithelial cell edema, and no tubular necrosis. Conclusion DRSAb has protective effects against renal ischemia/reperfusion injury in rats, which is associated with the activiation of PI3K/AK and PKCε signalling pathways.

Key words: Ischemia/reperfusion, Na+/K+-ATPase, DR region specific antibody, PI3K/AKT, PKCε

CLC Number: 

  • R392.7
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