Journal of Shandong University (Health Sciences) ›› 2019, Vol. 57 ›› Issue (9): 69-73.doi: 10.6040/j.issn.1671-7554.0.2019.586

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Correlation between human gastric cancer mesenchymal stem cells and tumor progression in SGC-7901 tumor-bearing mice

ZHAO Hongjian1, ZHOU Jijun1, SU Qingliang1, ZHAO Shuo1, LI Yuming2   

  1. 1. Department of General Surgery, Chengwu Hospital, Qianfoshan Hospital Group, Chengwu 274200, Shandong, China;
    2. Department of Gastrointestinal Surgery, Binzhou Medical University Affiliated Hospital, Binzhou 256600, Shandong, China
  • Published:2022-09-27

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Abstract: Objective To investigate the effects of human gastric cancer mesenchymal stem cells(hGC-MSCs)on the occurrence and development of gastric cancer cell line SGC-7901 in vivo. Methods The nude mice were divided into hGC-MSCs group, SGC-7901 group and hGC-MSCs+SGC-7901 group. The cell suspension was inoculated subcutaneously on the right back of the experimental mice to observe the occurrence of tumor, time of occurrence and change of tumor size. The expression levels of proliferating cell nuclear antigen(PCNA)and matrix metalloproteinase-9(MMP-9)were detected with immunohistochemical staining, real-time polymerase chain reaction(RT-PCR)and Western blotting. Results No tumor occurred in the control group. The hGC-MSCs+SGC-7901 group had shorter time of tumor occurrence, faster tumor growth and larger tumor size than the SGC-7901 group (P<0.05). The immunohistochemistry results showed that the average optical density of PCNA in SGC-7901 group and hGC-MSCs+SGC-7901 group was 0.358±0.009 and 0.481±0.030, respectively; the average optical density of MMP-9 in these two groups was 0.359±0.033 and 0.520±0.038, respectively (P<0.05). Real-time PCR demonstrated that hGC-MSCs could upregulate 山 东 大 学 学 报 (医 学 版)57卷9期 -赵鸿渐,等.胃癌间充质干细胞与SGC-7901荷瘤鼠肿瘤荷瘤鼠肿瘤进展的相关性研究 \=-the expressions of PCNA and MMP-9 (P<0.01). Western blotting showed that the PCNA protein band grayscale in SGC-7901 group and hGC-MSCs+SGC-7901 group was 0.756±0.039 and 0.847±0.048, respectively; the MMP-9 protein band grayscale in these two groups was 0.692±0.079 and 0.866±0.010, respectively (P<0.05). Conclusion HGC-MSCs participate in the occurrence and development of tumors by up-regulating the expression levels of PCNA and MMP-9 in vivo.

Key words: Gastric cancer, Mesenchymal stem cells, Proliferating cell nuclear antigen, Matrix metalloproteinase

CLC Number: 

  • R574
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