Journal of Shandong University (Health Sciences) ›› 2019, Vol. 57 ›› Issue (3): 1-6.doi: 10.6040/j.issn.1671-7554.0.2018.1233

   

Effect of cisplatin on IL-6 expression in tumor mesenchymal stem cells and underlying mechanism of p38MAPK pathway

ZHANG Zhihui1, LIU Yanguo1, XIAO Fengqi1, MA Daoxin2, WANG Xiuwen1   

  1. 1.Department of Medical Oncology;
    2. Department of Hematology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Published:2022-09-27

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Abstract: Objective To investigate the effect of cisplatin(CDDP)on interleukin(IL)-6 expression in tumor mesenchymal stem cells(MSCs)and explore its possible mechanisms. Methods Lewis lung carcinoma(LLC)cells or MSCs were treated with CDDP, and their IL gene expression was detected by polymerase chain reaction array(PCR Array)and further verified by quantitative real-time polymerase chain reaction(qRT-PCR)and enzyme-linked immunosorbent assay(ELISA). After CDDP treatment, the activation of p38MAPK pathway in MSCs was analyzed by Western blotting. CDDP activated p38MAPK pathway was inhibited by the specific inhibitor, and IL-6 expression in MSCs was measured by ELISA. Results CDDP treatment showed no significant effect on the expression of IL-6 in LLC cells(P>0.05). However, IL gene expression was changed in MSCs treated with CDDP for 24 h, and IL-6 expression was increased most significantly. Further studies confirmed the increase of IL-6 mRNA and protein in MSCs after CDDP treatment for 3 h, 6 h and 24 h(P<0.05). Higher expressions of p38 and pp38 were detected in MSCs after CDDP treatment. Moreover, the ratio of pp38/p38 was significantly increased. After the p38MAPK pathway was inhibited by SB203580, the up-regulation of IL-6 caused by CDDP was partially reversed. Conclusion CDDP has no significant effect on IL-6 expression in LLC cells, but it significantly up-regulates the expression of IL-6 in MSCs. CDDP treatment activates the p38MAPK pathway in MSCs, and inhibiting this pathway resultes in down-regulation of IL-6. These results indicate that CDDP might regulate the expression of IL-6 in MSCs via the p38MAPK pathway.

Key words: Lung cancer, Cisplatin, Interleukin-6, Mesenchymal stem cells, p38MAPK pathway

CLC Number: 

  • R734.2
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