Journal of Shandong University (Health Sciences) ›› 2021, Vol. 59 ›› Issue (4): 79-86.doi: 10.6040/j.issn.1671-7554.0.2021.0199

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Identification of differentially expressed genes and Wilms tumor-1 expression in adenomyosis

GENG Chen, YANG Yang, ZHAO Yue, LIU Haoran, CHAO Lan   

  1. Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250012, Shandong, China
  • Published:2021-04-30

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Abstract: Objective To screen the differentially expressed genes(DEGs)in adenomyosis from the Gene Expression Omnibus(GEO)database and to explore the expression of Wilms tumor-1(WT1). Methods The candidate genes were screened in the GEO database. The DEGs underwent Kyoto Encyclopedia of Genes and Genomes(KEGG)and Gene Ontology(GO)enrichment. The protein and mRNA expressions of WT1 in adenomyosis group and control group were detected with Western blotting and real-time quantitative polymerase chain reaction(qRT-PCR), respectively. The expression position and expression of WT1 in adenomyosis group and control group were determined with immunohistochemical staining. Results KEGG and GO of DEGs were enriched in transcription factor activity, cadherin binding involved in cell-cell adhesion, growth factor binding and other processes. Western blotting and qRT-PCR showed that the protein and mRNA expressions of WT1 were lower in the adenomyosis group than in the control group(P=0.002, P=0.003). Immunohistochemical staining showed that the WT1 staining level in stroma was lower in the adenomyosis group than in the control group(P=0.001), while the WT1 staining level in epithelium was higher(P<0.001); the overall staining level of WT1 in the adenomyosis group was lower than that in the control group(P=0.003). Conclusion The DEGs are screened from the GEO database and underwent KEGG and GO enrichment and WT1 may be involved in the pathogenesis of adenomyosis.

Key words: Adenomyosis, GEO data analysis, Differentially expressed genes, Enrichment analysis, Wilms tumor-1 gene

CLC Number: 

  • R711.71
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