Journal of Shandong University (Health Sciences) ›› 2021, Vol. 59 ›› Issue (4): 70-78.doi: 10.6040/j.issn.1671-7554.0.2021.0166

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Effects of lncRNA AC012073.1 on the migration and invasion of human breast cancer cells and its clinical significance

KONG Xue1,2, LI Juan1,2, DUAN Weili1,2, SHI Shuang1,2, LI Peilong1,2, DU Lutao1,2, MAO Haiting1, WANG Chuanxin1,2   

  • Published:2021-04-30

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Abstract: Objective To explore the expression of long non-coding RNA(lncRNA)AC012073.1 and its effects on the migration and invasion of human breast cancer(BC)cells. Methods The lncRNAs which were upregulated in BC tissues and related to poor prognosis were screened with lncRNA microarray and the cancer genome atlas(TCGA). The expression of AC012073.1 in BC cells and serum was detected with quantitative real-time PCR(qRT-PCR). MDA-MB-231 and MCF-7 cells were transfected with AC012073.1 siRNAs or plasmid and the transfection efficiency was detected with qRT-PCR. Cell migration and invasion were then determined with transwell assay and wound healing assay, respectively. The downstream targets and function enrichment analysis were predicted with TargetScan, and a regulatory network of ceRNA was constructed with Cytoscape software. The diagnostic efficacy of serum AC012073.1 for BC was analyzed with receiver operating characteristic(ROC)curve. Results A novel lncRNA, AC012073.1, was identified, which was significantly upregulated in BC tissues(P<0.001)and associated with poor prognosis(P=0.031). Compared with normal breast epithelial cells, BC cell lines had elevated expression of AC012073.1(P<0.05). Knockdown of AC012073.1 significantly inhibited cell migration and invasion, but overexpression of AC012073.1 promoted cell migration and invasion. The downstream targets and gene ontology(GO)analysis indicated that AC012073.1 promoted BC progression by regulating typical tumor signaling pathways. The qRT-PCR results showed the expression of AC012073.1 significantly increased in BC serum samples(P<0.001). The area under ROC curve(AUC)was 0.833, indicating it had a great diagnostic value for BC. Conclusion Highly expressed in BC, AC012073.1 promotes the migration and invasion of BC cells, and may serve as a potential biomarker for the diagnosis and prognosis of BC.

Key words: LncRNA, Breast cancer, Migration, Invasion, Biomarker

CLC Number: 

  • R737.9
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