JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (9): 41-47.doi: 10.6040/j.issn.1671-7554.0.2015.898

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Effect of osteopontin through NF-kappaB signaling pathway in the temporomandibular joint of rats subjected to chronic sleep deprivation

DING Feng1, DONG Yabing2, ZHAO Huaqiang2, ZHU Guoxiong3, WU Gaoyi3   

  1. 1. Department of Stomatology, Jinan Military General Hospital of Jinzhou Medical University, Jinan 250031, Shandong, China;
    2. School of Stomatology of Shandong University, Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Jinan 250012, Shandong, China;
    3. Department of Stomatology, Jinan Military General Hospital, Jinan 250031, Shandong, China
  • Received:2015-09-22 Online:2016-09-10 Published:2016-09-10

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Abstract: Objective To explore the effect of osteopontin(OPN)in the temporomandibular joint(TMJ)of rats subjected to chronic sleep deprivation(CSD). Methods Rats were subjected to CSD using the modified multiple platform method(MMPM). A total of 180 male 8-week-old Wistar rats were randomly divided into two groups(n=90 per group): the control(CON)group and chronic sleep deprivation(CSD)group. The two groups were equally divided into three subgroups(n=30 each)according to the observation time points(4, 6, and 8 weeks). The expression of OPN was measured with immunohistochemical method. The expression of NF-κB of TMJ was measured with immunohistochemical method and immunofluorescence. The relationship between the expressions of OPN and NF-κB and CSD extension was analyzed. Results Hematoxylin and eosin(HE)staining showed pathological alterations in the TMJ in the CSD group. With the extension of CSD, the injury on cartilage was exacerbated. The expressions of OPN and 山 东 大 学 学 报 (医 学 版)54卷9期 -丁凤,等.在慢性睡眠剥夺中骨桥蛋白通过NF-kappaB通路对颞下颌关节的影响 \=-NF-κB in the CSD groups were significantly higher than those in the control group(P<0.05). The expressions of OPN and NF-κB had a rising tendency with the extension of CSD. Conclusion CSD causes pathological alterations in the TMJ condylar cartilages by the expression of OPN from rat condylar cartilage chondrocytes and various inflammatory cells. OPN aggravates the pathological changes in the TMJ by activating the NF-κB signaling pathway.

Key words: Temporomandibular joint, Chronic sleep deprivation, Osteopontin, Nuclear factor-kappa B

CLC Number: 

  • R246.83
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