JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (6): 50-54.doi: 10.6040/j.issn.1671-7554.0.2015.1222

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Expression of BDNF/TrkB and effect of BDNF on the proliferation ability of tongue squamous cell carcinoma

WEI Xiujuan1, WU Xiuyin2, TONG Dongdong1, LI Jing1, YANG Xiaolu1, ZHANG Fenghe1   

  1. 1. Department of Oral and Maxillofacial Surgery, Stomatological Hospital of Shandong University, Jinan 250012, Shandong, China;
    2. Department of Stomatology, Peoples Hospital of Laiwu City, Laiwu 271100, Shandong, China
  • Received:2015-12-03 Online:2016-06-20 Published:2016-06-20

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Abstract: Objective To investigate the expressions of brain-derived neurotrophic factor(BDNF)and its primary receptor tyrosine kinase receptor B(TrkB)in tongue squamous cell carcinoma(TSCC), and to explore the effect of BDNF on the proliferation of TSCC. Methods The expressions of BDNF and TrkB in 50 TSCC and 13 normal mucosa tissues were detected with immunohistochemical method. The expressions of BDNF and TrkB in human tongue squamous cell carcinoma cell lines(Tca8113)were examined with immunofluorescence technique. Cell viability of Tca8113 was detected with CCK8 assay. Results The positive rate of staining of BDNF and TrkB in TSCC was significantly higher than that in normal tongue mucosa(P<0.05). In addition, the rate of positive expression was related to infiltration depth and lymph node metastasis. BDNF(200 ng/mL)significantly promoted the proliferation of Tca8113. Conclusion BDNF and TrkB may be closely associated with the genesis and development of TSCC. Moreover, BDNF may promote cell proliferation of TSCC.

Key words: Brain-derived neurotrophic factor, Tyrosine kinase receptor B, Tongue squamous cell carcinoma, Proliferation ability

CLC Number: 

  • R782
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