JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (5): 55-59.doi: 10.6040/j.issn.1671-7554.0.2014.881

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Effects of miR-17 on the senescence of human foreskin fibroblasts

CHEN Yan, LIU Juan, CHEN Hanxiang, ZHANG Weifang, ZHAO Weiming   

  1. Department of Pathogenic Biology, School of Medicine, Shandong University, Jinan 250012, Shandong, China
  • Received:2014-11-27 Revised:2015-01-20 Online:2015-05-10 Published:2015-05-10

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Abstract: Objective To study the effect of miR-17 on the senescence of human foreskin fibroblasts (HFF). Methods Recombinant lentivirus-expressed miR-17 infected HFF and miR-17 expression was examined with qRT-PCR. The proliferation and senescence of HFF were detected with CCK-8 and SA-β-Gal kit, respectively. Cell cycle profile was observed with flow cytometry. The protein levels of cyclin D1 and p21 were detected with Western blotting. Results HFF were successfully isolated. Compared with HFF-NC, the proliferation ability of HFF-miR-17 cells was obviously increased while the expression of SA-β-Gal in senescent cells decreased. S phase cells of HFF-miR-17 increased significantly and more HFF-NC cells arrested in the G1 phase. Expression of cyclin D1 was significantly up-regulated in HFF-miR-17, while p21 expression was attenuated. Conclusion miR-17 promotes the proliferation and inhibits senescence of primary cells by upregulating cyclin D1 and downregulating p21 proteins.

Key words: miR-17, Human foreskin fibroblasts, Cell cycle, Senescence

CLC Number: 

  • R392.2
[1] Kuilman T, Michaloglou C, Mooi WJ, et al. The essence of senescence[J]. Gene Dev, 2010, 24(22): 2463-2479.
[2] Bartel DP. MicroRNAs: genomics, biogenesis, mechanism, and, function[J]. Cell, 2004, 116(2): 281-297.
[3] Jasinski-Bergner S, Mandelboim O, Seliger B. The role of microRNAs in the control of innate immune response in cancer[J]. J Natl Cancer Inst, 2014, 106(10). pii: dju257. doi: 10.1093/jnci/dju257. Print 2014 Oct.
[4] Gama-Carvalho M, Andrade J, Brás-Rosário L. Regulation of cardiac cell fate by microRNAs: implications for Heart Regeneration[J]. Cells, 2014, 3(4): 996-1026.
[5] Ma Y, Yang HZ, Dong BJ, et al. Biphasic regulation of autophagy by miR-96 in prostate cancer cells under hypoxia[J]. Oncotarget, 2014, 5(19): 9169-9182.
[6] Guo J, Feng Z, Huang Z, et al. MicroRNA-217 functions as a tumour suppressor gene and correlates with cell resistance to cisplatin in lung cancer[J]. Mol Cells, 2014, 37(9): 664-671.
[7] Andorfer CA, Necela BM, Thompson EA, et al. MicroRNA signatures: clinical biomarkers for the diagnosis and treatment of breast cancer[J]. Trends Mol Med, 2011, 17(6): 313-319.
[8] Bonauer A, Dimmeler S. The microRNA-17-92 cluster still amiracle?[J]. Cell Cycle, 2009, 23(8): 3866-3873.
[9] Hackl M, Brunner S, Fortschegger K, et al. mirR-17, mirR-19b, mirR-20a, and mirR-106a are down-regulated in human aging[J]. Aging cell, 2010, 9(2): 291-296.
[10] Campisi J. Aging, cellular senescence, and cancer[J]. Annu Rev Physiol, 2013, 75: 685-705.
[11] Li G, Luna C, Qiu J, et al. Alterations in microRNA Expression in Stress-induced Cellular Senescence[J]. Mech Ageing Dev, 2009, 130(11-12): 731-741.
[12] Wang M, Cheng Z, Tian T, et al. Differential expression of oncogenicmiRNAs in proliferating and senescent human fibroblasts[J]. Mol Cell Biochem, 2011, 352 (1-2): 271-279.
[13] Klein EA, Assoian RK. Transcriptional regulation of the cyclin D1 gene at a glance[J]. J Cell Sci, 2008, 121(23): 3853-3857
[14] Harper JW, Adami GR, Wei N, et al. The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases[J]. Cell, 1993, 75(4): 805-816.
[15] 田兴松, 王新刚, 周文红, 等. 乳腺癌细胞中p16、p21、cyclin D1的表达及临床意义[J]. 山东大学学报: 医学版, 2005, 43(6): 532-543. TIAN Xingsong, WANG Xingang, ZHOU Wenhong, et al. Expressions of p16, p21, and cyclin D1 in breast cancer cells and their clinical significance[J]. Journal of Shandong University: Health Sciences, 2005, 43(6): 532-543.
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